Experiment: Lambda_ci1857
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slyB and slyA are separated by 46 nucleotides slyA and ydhI are separated by 200 nucleotides ydhI and b1644 are separated by 2 nucleotides b1644 and ydhK overlap by 1 nucleotides
b1641: slyB - outer membrane lipoprotein (NCBI), at 1,717,900 to 1,718,367
slyB
b1642: slyA - transcriptional regulator for cryptic hemolysin (VIMSS), at 1,718,414 to 1,718,848
slyA
b1643: ydhI - predicted inner membrane protein (NCBI), at 1,719,049 to 1,719,285
ydhI
b1644: b1644 - putative membrane protein (VIMSS), at 1,719,288 to 1,720,145
b1644
b1645: ydhK - conserved inner membrane protein (NCBI), at 1,720,145 to 1,722,157
ydhK
Position (kb)
1719
1720
1721 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1718.718 kb on - strand, within slyA at 1718.718 kb on - strand, within slyA at 1718.809 kb on - strand at 1719.062 kb on - strand at 1719.065 kb on + strand at 1719.078 kb on + strand, within ydhI at 1719.103 kb on - strand, within ydhI at 1719.163 kb on + strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.190 kb on + strand, within ydhI at 1719.211 kb on + strand, within ydhI at 1719.221 kb on - strand, within ydhI at 1719.559 kb on - strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644 at 1720.086 kb on + strand at 1720.196 kb on - strand at 1720.263 kb on + strand at 1720.263 kb on + strand at 1720.388 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.520 kb on + strand, within ydhK at 1720.587 kb on - strand, within ydhK at 1720.587 kb on - strand, within ydhK at 1720.615 kb on + strand, within ydhK at 1720.741 kb on + strand, within ydhK at 1721.132 kb on + strand, within ydhK at 1721.142 kb on - strand, within ydhK at 1721.142 kb on - strand, within ydhK
Per-strain Table
Position Strand Gene LocusTag Fraction Lambda_ci1857 remove 1,718,718 - slyA b1642 0.70 +0.4 1,718,718 - slyA b1642 0.70 -0.1 1,718,809 - -2.2 1,719,062 - -0.0 1,719,065 + +2.8 1,719,078 + ydhI b1643 0.12 +1.3 1,719,103 - ydhI b1643 0.23 -0.6 1,719,163 + ydhI b1643 0.48 -2.2 1,719,171 - ydhI b1643 0.51 -0.7 1,719,171 - ydhI b1643 0.51 +0.0 1,719,190 + ydhI b1643 0.59 -0.6 1,719,211 + ydhI b1643 0.68 -0.6 1,719,221 - ydhI b1643 0.73 -1.2 1,719,559 - b1644 b1644 0.32 -1.1 1,719,779 + b1644 b1644 0.57 -1.4 1,719,779 + b1644 b1644 0.57 -0.2 1,719,786 - b1644 b1644 0.58 -0.7 1,719,786 - b1644 b1644 0.58 -1.5 1,719,832 - b1644 b1644 0.63 -2.0 1,719,832 - b1644 b1644 0.63 +1.0 1,720,086 + +0.4 1,720,196 - -0.6 1,720,263 + +0.6 1,720,263 + -0.0 1,720,388 + ydhK b1645 0.12 +1.0 1,720,466 + ydhK b1645 0.16 +0.3 1,720,466 + ydhK b1645 0.16 -1.4 1,720,466 + ydhK b1645 0.16 -1.1 1,720,466 + ydhK b1645 0.16 -1.4 1,720,520 + ydhK b1645 0.19 -1.1 1,720,587 - ydhK b1645 0.22 -1.5 1,720,587 - ydhK b1645 0.22 +0.5 1,720,615 + ydhK b1645 0.23 -0.0 1,720,741 + ydhK b1645 0.30 +1.0 1,721,132 + ydhK b1645 0.49 -0.7 1,721,142 - ydhK b1645 0.50 -1.4 1,721,142 - ydhK b1645 0.50 +0.3
Or see this region's nucleotide sequence