Strain Fitness in Escherichia coli BW25113 around b3370

Experiment: LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector
3,496,942	+	cysG	b3368	0.79	-0.1
3,496,942	+	cysG	b3368	0.79	-0.5
3,496,947	+	cysG	b3368	0.80	-0.6
3,496,960	-	cysG	b3368	0.81	-0.2
3,496,981	+	cysG	b3368	0.82	-0.9
3,496,981	+	cysG	b3368	0.82	-0.1
3,496,986	+	cysG	b3368	0.83	-0.7
3,496,986	+	cysG	b3368	0.83	-1.1
3,496,986	+	cysG	b3368	0.83	-0.4
3,496,986	+	cysG	b3368	0.83	-0.5
3,496,988	+	cysG	b3368	0.83	-0.7
3,496,989	+	cysG	b3368	0.83	-0.3
3,496,989	+	cysG	b3368	0.83	-0.1
3,496,989	+	cysG	b3368	0.83	-0.3
3,496,991	+	cysG	b3368	0.83	-0.2
3,496,994	-	cysG	b3368	0.83	+1.0
3,496,994	-	cysG	b3368	0.83	-0.9
3,496,994	-	cysG	b3368	0.83	+1.4
3,496,996	+	cysG	b3368	0.83	-0.7
3,496,996	+	cysG	b3368	0.83	-0.9
3,497,012	+	cysG	b3368	0.85	-0.9
3,497,017	+	cysG	b3368	0.85	-0.4
3,497,054	+	cysG	b3368	0.88	-0.8
3,497,089	+				-0.8
3,497,097	+				-1.9
3,497,097	+				-0.1
3,497,113	+				-1.4
3,497,114	+				-0.5
3,497,114	+				-0.6
3,497,114	+				-0.9
3,497,114	+				-0.2
3,497,114	+				-0.7
3,497,114	+				-0.4
3,497,116	+				+0.1
3,497,117	+				-0.5
3,497,118	+				-0.6
3,497,124	+				-0.3
3,497,124	+				-0.6
3,497,124	+				-1.0
3,497,130	-				+1.4
3,497,136	+				-0.2
3,497,142	+				-1.4
3,497,142	+				-0.9
3,497,143	+				-0.3
3,497,143	+				-1.0
3,497,146	+				+0.0
3,497,159	+				-0.9
3,497,165	+				+0.3
3,497,172	+				-1.6
3,497,173	+				-1.0
3,497,173	+				-0.5
3,497,180	+				-0.4
3,497,180	+				-0.7
3,497,180	+				-0.6
3,497,180	+				-0.7
3,497,190	+				-0.2
3,497,192	+				+0.0
3,497,192	+				+0.3
3,497,192	+				+0.0
3,497,192	+				+1.5
3,497,192	+				+0.3
3,497,192	+				+0.1
3,497,192	+				-0.4
3,497,193	+				+0.7
3,497,205	+				+0.6
3,497,205	+				+0.4
3,497,205	+				+0.2
3,497,213	+				+0.0
3,497,454	-				+0.3
3,497,454	-				-1.0
3,497,494	+	yhfL	b3369	0.14	+1.4
3,497,497	+	yhfL	b3369	0.16	+1.1
3,497,501	+	yhfL	b3369	0.18	-1.8
3,497,501	+	yhfL	b3369	0.18	+0.1
3,497,504	-	yhfL	b3369	0.20	-0.8
3,497,504	-	yhfL	b3369	0.20	+0.5
3,497,506	+	yhfL	b3369	0.21	+0.8
3,497,506	-	yhfL	b3369	0.21	+0.6
3,497,508	+	yhfL	b3369	0.23	-0.2
3,497,508	+	yhfL	b3369	0.23	-0.2
3,497,509	-	yhfL	b3369	0.23	+0.7
3,497,509	-	yhfL	b3369	0.23	+0.2
3,497,509	-	yhfL	b3369	0.23	+0.7
3,497,511	+	yhfL	b3369	0.24	+0.6
3,497,514	-	yhfL	b3369	0.26	+0.1
3,497,514	-	yhfL	b3369	0.26	-0.8
3,497,514	-	yhfL	b3369	0.26	-1.2
3,497,514	-	yhfL	b3369	0.26	-1.4
3,497,514	-	yhfL	b3369	0.26	-0.7
3,497,514	-	yhfL	b3369	0.26	-0.6
3,497,514	-	yhfL	b3369	0.26	+0.3
3,497,514	-	yhfL	b3369	0.26	-0.8
3,497,514	-	yhfL	b3369	0.26	-0.2
3,497,516	+	yhfL	b3369	0.27	+1.4
3,497,516	+	yhfL	b3369	0.27	+1.2
3,497,518	-	yhfL	b3369	0.29	+0.7
3,497,518	-	yhfL	b3369	0.29	+0.8
3,497,518	-	yhfL	b3369	0.29	-0.1
3,497,518	-	yhfL	b3369	0.29	-0.8
3,497,519	-	yhfL	b3369	0.29	-0.6
3,497,519	-	yhfL	b3369	0.29	-0.9
3,497,519	-	yhfL	b3369	0.29	-0.5
3,497,522	+	yhfL	b3369	0.31	+0.1
3,497,522	+	yhfL	b3369	0.31	+0.2
3,497,522	+	yhfL	b3369	0.31	+0.6
3,497,524	-	yhfL	b3369	0.32	+0.2
3,497,524	-	yhfL	b3369	0.32	-0.4
3,497,524	-	yhfL	b3369	0.32	+0.6
3,497,524	-	yhfL	b3369	0.32	+1.2
3,497,524	-	yhfL	b3369	0.32	-0.9
3,497,527	-	yhfL	b3369	0.34	+0.1
3,497,527	-	yhfL	b3369	0.34	+0.2
3,497,531	-	yhfL	b3369	0.36	+0.9
3,497,531	-	yhfL	b3369	0.36	-1.3
3,497,534	-	yhfL	b3369	0.38	-0.3
3,497,534	-	yhfL	b3369	0.38	-0.9
3,497,536	-	yhfL	b3369	0.39	+0.0
3,497,538	-	yhfL	b3369	0.40	-0.1
3,497,547	-	yhfL	b3369	0.46	+0.4
3,497,556	+	yhfL	b3369	0.51	-0.8
3,497,559	-	yhfL	b3369	0.53	-0.0
3,497,562	+	yhfL	b3369	0.55	+0.8
3,497,563	+	yhfL	b3369	0.55	-0.6
3,497,563	+	yhfL	b3369	0.55	-0.5
3,497,563	+	yhfL	b3369	0.55	-0.1
3,497,567	+	yhfL	b3369	0.58	-0.0
3,497,569	-	yhfL	b3369	0.59	+0.6
3,497,571	-	yhfL	b3369	0.60	-2.4
3,497,571	-	yhfL	b3369	0.60	-0.2
3,497,571	-	yhfL	b3369	0.60	-0.1
3,497,572	-	yhfL	b3369	0.61	-0.5
3,497,575	+	yhfL	b3369	0.62	+0.4
3,497,575	+	yhfL	b3369	0.62	+0.2
3,497,580	-	yhfL	b3369	0.65	+0.3
3,497,580	-	yhfL	b3369	0.65	+0.4
3,497,580	-	yhfL	b3369	0.65	-0.7
3,497,583	-	yhfL	b3369	0.67	-0.2
3,497,619	+	yhfL	b3369	0.89	-2.2
3,497,621	+				+0.2
3,497,624	+				+0.2
3,497,627	+				+0.5
3,497,627	-				-0.5
3,497,629	+				-0.2
3,497,629	+				+0.2
3,497,629	-				+0.2
3,497,640	-				-0.8
3,497,711	-				+0.2
3,497,777	-				+0.0
3,497,784	-				-0.4
3,497,784	-				+0.2
3,497,788	+				+0.3
3,497,788	+				-0.6
3,497,801	-				-0.2
3,497,896	+				+0.1
3,497,904	-				-0.2
3,497,907	-				+0.1
3,497,937	-				+1.0
3,497,939	+				+0.8
3,497,939	+				+0.2
3,498,022	-				-0.1
3,498,025	+				-0.1
3,498,025	+				-0.5
3,498,034	-				-0.0
3,498,034	-				+0.6
3,498,048	+				-0.1
3,498,059	+				-0.3
3,498,059	+				-0.5
3,498,059	+				+0.4
3,498,061	-				+0.3
3,498,067	-	yhfM	b3370	0.10	-0.1
3,498,067	-	yhfM	b3370	0.10	-0.0
3,498,077	-	yhfM	b3370	0.11	-0.6
3,498,077	-	yhfM	b3370	0.11	+0.0
3,498,085	+	yhfM	b3370	0.11	+1.0
3,498,089	+	yhfM	b3370	0.12	-0.3
3,498,111	-	yhfM	b3370	0.13	-0.3
3,498,119	+	yhfM	b3370	0.14	-0.2
3,498,129	+	yhfM	b3370	0.15	+0.1
3,498,129	+	yhfM	b3370	0.15	-0.4
3,498,137	-	yhfM	b3370	0.15	+2.1
3,498,137	-	yhfM	b3370	0.15	+1.2
3,498,157	+	yhfM	b3370	0.17	-0.8
3,498,196	+	yhfM	b3370	0.20	-0.6
3,498,196	+	yhfM	b3370	0.20	-0.4
3,498,203	+	yhfM	b3370	0.20	-0.2
3,498,214	+	yhfM	b3370	0.21	+0.1
3,498,248	-	yhfM	b3370	0.24	+0.6
3,498,258	-	yhfM	b3370	0.24	-0.8
3,498,258	-	yhfM	b3370	0.24	-0.4
3,498,261	+	yhfM	b3370	0.25	-0.1
3,498,261	+	yhfM	b3370	0.25	+0.0
3,498,285	+	yhfM	b3370	0.26	+0.3
3,498,349	+	yhfM	b3370	0.31	+0.0
3,498,351	+	yhfM	b3370	0.31	-0.5
3,498,351	+	yhfM	b3370	0.31	+0.4
3,498,365	-	yhfM	b3370	0.32	+0.3
3,498,365	-	yhfM	b3370	0.32	+0.5
3,498,365	-	yhfM	b3370	0.32	-0.4
3,498,375	-	yhfM	b3370	0.33	+0.1
3,498,385	+	yhfM	b3370	0.34	-0.1
3,498,393	-	yhfM	b3370	0.34	-0.7
3,498,446	-	yhfM	b3370	0.38	+0.3
3,498,446	-	yhfM	b3370	0.38	+0.1
3,498,517	-	yhfM	b3370	0.44	+0.7
3,498,535	+	yhfM	b3370	0.45	-0.0
3,498,543	-	yhfM	b3370	0.46	-1.0
3,498,543	-	yhfM	b3370	0.46	+0.1
3,498,549	+	yhfM	b3370	0.46	-0.0
3,498,549	+	yhfM	b3370	0.46	+0.2
3,498,575	+	yhfM	b3370	0.48	-0.1
3,498,620	-	yhfM	b3370	0.51	+0.2
3,498,620	-	yhfM	b3370	0.51	-0.3
3,498,640	-	yhfM	b3370	0.53	-0.2
3,498,655	+	yhfM	b3370	0.54	-0.1
3,498,655	+	yhfM	b3370	0.54	-0.5
3,498,655	+	yhfM	b3370	0.54	-0.3
3,498,664	+	yhfM	b3370	0.55	-0.2
3,498,664	+	yhfM	b3370	0.55	-0.1
3,498,667	-	yhfM	b3370	0.55	+0.3
3,498,667	-	yhfM	b3370	0.55	-0.1
3,498,670	-	yhfM	b3370	0.55	-0.4
3,498,749	-	yhfM	b3370	0.61	+0.8
3,498,827	-	yhfM	b3370	0.67	-0.1
3,498,827	-	yhfM	b3370	0.67	+0.1
3,498,837	-	yhfM	b3370	0.68	+0.1
3,498,840	-	yhfM	b3370	0.68	-0.6
3,498,850	+	yhfM	b3370	0.69	-0.8
3,498,850	+	yhfM	b3370	0.69	-0.3
3,498,892	+	yhfM	b3370	0.72	-0.0
3,498,892	+	yhfM	b3370	0.72	+0.4
3,498,900	-	yhfM	b3370	0.72	-0.3
3,498,941	+	yhfM	b3370	0.75	-0.6
3,498,952	+	yhfM	b3370	0.76	+0.0
3,498,966	-	yhfM	b3370	0.77	+0.2
3,498,981	+	yhfM	b3370	0.78	+1.0
3,498,988	-	yhfM	b3370	0.79	+0.5
3,498,998	+	yhfM	b3370	0.80	-0.0
3,498,998	+	yhfM	b3370	0.80	-0.0
3,499,048	+	yhfM	b3370	0.83	-0.4
3,499,048	+	yhfM	b3370	0.83	+0.2
3,499,057	+	yhfM	b3370	0.84	-0.3
3,499,085	+	yhfM	b3370	0.86	-0.1
3,499,180	-				-0.6
3,499,213	-				-0.7
3,499,213	-				+0.3
3,499,213	-				+0.6
3,499,263	+				-0.0
3,499,263	+				-0.7
3,499,395	+	yhfN	b3371	0.10	+0.3
3,499,398	+	yhfN	b3371	0.11	+1.2
3,499,398	+	yhfN	b3371	0.11	-0.0
3,499,401	-	yhfN	b3371	0.11	+0.2
3,499,462	-	yhfN	b3371	0.17	-0.3
3,499,508	+	yhfN	b3371	0.21	+0.8
3,499,508	+	yhfN	b3371	0.21	+0.3
3,499,532	+	yhfN	b3371	0.24	-0.5
3,499,645	-	yhfN	b3371	0.35	-0.5
3,499,664	+	yhfN	b3371	0.37	-0.2
3,499,675	+	yhfN	b3371	0.38	+0.5
3,499,762	-	yhfN	b3371	0.46	-0.3
3,499,762	-	yhfN	b3371	0.46	+0.7
3,499,767	+	yhfN	b3371	0.47	+0.5
3,499,767	+	yhfN	b3371	0.47	-1.1
3,499,775	-	yhfN	b3371	0.47	-1.1
3,499,899	-	yhfN	b3371	0.60	-0.4
3,499,914	-	yhfN	b3371	0.61	-0.1
3,499,929	-	yhfN	b3371	0.62	-0.6
3,499,935	-	yhfN	b3371	0.63	-0.6
3,499,950	+	yhfN	b3371	0.65	-0.4
3,499,982	+	yhfN	b3371	0.68	+0.6
3,500,052	+	yhfN	b3371	0.74	+0.3
3,500,136	-	yhfN	b3371	0.83	+0.1
3,500,192	+	yhfN	b3371	0.88	+0.1
3,500,237	-				+0.6
3,500,237	-				-1.8
3,500,239	+				-0.1
3,500,245	-				+1.0