Experiment: LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaiV and ampH are separated by 0 nucleotides
b0375: yaiV - predicted DNA-binding transcriptional regulator (RefSeq), at 393,730 to 394,353
yaiV
b0376: ampH - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 394,354 to 395,511
ampH
Position (kb) 393
394
395 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 392.747 kb on + strand at 392.829 kb on - strand at 392.831 kb on + strand at 393.017 kb on - strand at 393.175 kb on - strand at 393.175 kb on - strand at 393.200 kb on - strand at 393.285 kb on + strand at 393.432 kb on + strand at 393.432 kb on + strand at 393.439 kb on + strand at 393.447 kb on - strand at 393.492 kb on - strand at 393.492 kb on - strand at 393.494 kb on + strand at 393.504 kb on + strand at 393.504 kb on - strand at 393.511 kb on + strand at 393.511 kb on + strand at 393.523 kb on - strand at 393.523 kb on - strand at 393.523 kb on - strand at 393.557 kb on + strand at 393.560 kb on + strand at 393.560 kb on + strand at 393.564 kb on + strand at 393.564 kb on + strand at 393.569 kb on + strand at 393.569 kb on + strand at 393.576 kb on + strand at 393.578 kb on + strand at 393.587 kb on + strand at 393.587 kb on + strand at 393.587 kb on + strand at 393.588 kb on + strand at 393.588 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.598 kb on + strand at 393.603 kb on + strand at 393.608 kb on + strand at 393.611 kb on + strand at 393.611 kb on + strand at 393.618 kb on + strand at 393.618 kb on + strand at 393.622 kb on + strand at 393.642 kb on + strand at 393.646 kb on + strand at 393.680 kb on + strand at 393.699 kb on + strand at 393.710 kb on + strand at 393.710 kb on + strand at 393.713 kb on + strand at 393.713 kb on - strand at 393.718 kb on - strand at 393.718 kb on - strand at 393.724 kb on + strand at 393.739 kb on + strand at 393.739 kb on - strand at 393.741 kb on + strand at 393.749 kb on - strand at 393.749 kb on - strand at 393.769 kb on + strand at 393.786 kb on + strand at 393.788 kb on + strand at 393.789 kb on + strand at 393.792 kb on + strand at 393.793 kb on + strand, within yaiV at 393.868 kb on - strand, within yaiV at 393.887 kb on - strand, within yaiV at 393.901 kb on - strand, within yaiV at 393.901 kb on - strand, within yaiV at 393.910 kb on - strand, within yaiV at 393.912 kb on + strand, within yaiV at 393.912 kb on + strand, within yaiV at 394.044 kb on - strand, within yaiV at 394.083 kb on - strand, within yaiV at 394.128 kb on + strand, within yaiV at 394.170 kb on - strand, within yaiV at 394.203 kb on + strand, within yaiV at 394.205 kb on + strand, within yaiV at 394.205 kb on + strand, within yaiV at 394.209 kb on - strand, within yaiV at 394.209 kb on - strand, within yaiV at 394.212 kb on - strand, within yaiV at 394.241 kb on - strand, within yaiV at 394.244 kb on - strand, within yaiV at 394.244 kb on - strand, within yaiV at 394.248 kb on - strand, within yaiV at 394.256 kb on - strand, within yaiV at 394.256 kb on - strand, within yaiV at 394.256 kb on - strand, within yaiV at 394.262 kb on + strand, within yaiV at 394.264 kb on + strand, within yaiV at 394.264 kb on + strand, within yaiV at 394.271 kb on + strand, within yaiV at 394.279 kb on - strand, within yaiV at 394.281 kb on + strand, within yaiV at 394.281 kb on + strand, within yaiV at 394.313 kb on - strand at 394.313 kb on - strand at 394.325 kb on + strand at 394.334 kb on - strand at 394.335 kb on + strand at 394.355 kb on - strand at 394.355 kb on - strand at 394.355 kb on - strand at 394.355 kb on - strand at 394.355 kb on - strand at 394.357 kb on - strand at 394.378 kb on - strand at 394.378 kb on - strand at 394.381 kb on + strand at 394.389 kb on - strand at 394.389 kb on - strand at 394.394 kb on - strand at 394.394 kb on - strand at 394.394 kb on - strand at 394.402 kb on - strand at 394.408 kb on + strand at 394.408 kb on + strand at 394.413 kb on - strand at 394.413 kb on - strand at 394.416 kb on - strand at 394.416 kb on - strand at 394.416 kb on - strand at 394.421 kb on - strand at 394.421 kb on - strand at 394.446 kb on - strand at 394.446 kb on - strand at 394.454 kb on - strand at 394.454 kb on - strand at 394.461 kb on - strand at 394.467 kb on - strand at 394.469 kb on - strand at 394.469 kb on - strand at 394.475 kb on + strand, within ampH at 394.475 kb on + strand, within ampH at 394.497 kb on + strand, within ampH at 394.502 kb on - strand, within ampH at 394.504 kb on + strand, within ampH at 394.505 kb on - strand, within ampH at 394.505 kb on - strand, within ampH at 394.542 kb on + strand, within ampH at 394.560 kb on - strand, within ampH at 394.565 kb on + strand, within ampH at 394.576 kb on - strand, within ampH at 394.576 kb on - strand, within ampH at 394.576 kb on - strand, within ampH at 394.582 kb on - strand, within ampH at 394.616 kb on - strand, within ampH at 394.619 kb on - strand, within ampH at 394.620 kb on - strand, within ampH at 394.628 kb on + strand, within ampH at 394.665 kb on + strand, within ampH at 394.665 kb on + strand, within ampH at 394.719 kb on - strand, within ampH at 394.850 kb on - strand, within ampH at 394.850 kb on - strand, within ampH at 394.853 kb on + strand, within ampH at 394.909 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.955 kb on + strand, within ampH at 394.956 kb on + strand, within ampH at 394.964 kb on - strand, within ampH at 394.964 kb on - strand, within ampH at 395.074 kb on - strand, within ampH at 395.200 kb on + strand, within ampH at 395.200 kb on + strand, within ampH at 395.208 kb on - strand, within ampH at 395.208 kb on - strand, within ampH at 395.223 kb on + strand, within ampH at 395.227 kb on + strand, within ampH at 395.227 kb on + strand, within ampH at 395.280 kb on - strand, within ampH at 395.298 kb on - strand, within ampH at 395.350 kb on + strand, within ampH
Per-strain Table
Position Strand Gene LocusTag Fraction LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector remove 392,747 + +0.2 392,829 - -3.2 392,831 + -1.4 393,017 - +0.0 393,175 - -0.3 393,175 - -0.2 393,200 - +0.4 393,285 + -0.2 393,432 + +0.2 393,432 + -0.4 393,439 + +0.1 393,447 - -0.2 393,492 - -1.4 393,492 - +0.4 393,494 + -0.4 393,504 + +0.2 393,504 - +0.4 393,511 + -0.0 393,511 + -0.4 393,523 - -0.5 393,523 - +0.9 393,523 - +0.2 393,557 + -0.1 393,560 + -0.2 393,560 + +0.2 393,564 + +0.1 393,564 + -0.3 393,569 + +0.3 393,569 + +1.8 393,576 + -0.5 393,578 + -0.2 393,587 + +0.2 393,587 + -0.3 393,587 + +0.3 393,588 + -0.1 393,588 + +0.2 393,598 + +0.3 393,598 + -0.3 393,598 + -0.0 393,598 + +0.4 393,598 + -0.3 393,598 + -0.1 393,598 + +0.2 393,598 + +0.2 393,598 + +0.7 393,598 + -0.2 393,598 + +0.1 393,598 + -0.5 393,598 + +0.1 393,598 + +0.2 393,598 + +0.1 393,598 + -0.2 393,598 + +0.2 393,598 + -0.2 393,598 + -0.1 393,598 + +0.2 393,598 + -0.0 393,598 + +0.7 393,598 + +0.1 393,598 + +0.1 393,598 + +0.7 393,598 + +0.1 393,603 + -0.8 393,608 + +0.4 393,611 + -0.2 393,611 + +0.8 393,618 + -0.3 393,618 + +0.1 393,622 + +0.7 393,642 + -0.2 393,646 + +0.0 393,680 + +0.4 393,699 + -1.6 393,710 + -1.9 393,710 + +1.4 393,713 + +0.4 393,713 - +1.4 393,718 - +1.4 393,718 - -0.2 393,724 + +0.4 393,739 + +1.4 393,739 - +0.1 393,741 + -0.6 393,749 - +2.4 393,749 - -0.4 393,769 + +1.4 393,786 + +1.4 393,788 + -0.2 393,789 + -0.2 393,792 + +0.4 393,793 + yaiV b0375 0.10 +0.4 393,868 - yaiV b0375 0.22 +0.8 393,887 - yaiV b0375 0.25 +2.0 393,901 - yaiV b0375 0.27 +1.4 393,901 - yaiV b0375 0.27 +2.1 393,910 - yaiV b0375 0.29 +1.4 393,912 + yaiV b0375 0.29 +1.4 393,912 + yaiV b0375 0.29 +1.4 394,044 - yaiV b0375 0.50 -0.9 394,083 - yaiV b0375 0.57 -1.9 394,128 + yaiV b0375 0.64 +0.1 394,170 - yaiV b0375 0.71 +1.4 394,203 + yaiV b0375 0.76 +2.4 394,205 + yaiV b0375 0.76 +2.0 394,205 + yaiV b0375 0.76 -2.1 394,209 - yaiV b0375 0.77 -2.1 394,209 - yaiV b0375 0.77 +0.0 394,212 - yaiV b0375 0.77 +1.7 394,241 - yaiV b0375 0.82 -0.9 394,244 - yaiV b0375 0.82 +0.4 394,244 - yaiV b0375 0.82 -0.4 394,248 - yaiV b0375 0.83 -2.1 394,256 - yaiV b0375 0.84 -0.5 394,256 - yaiV b0375 0.84 +0.7 394,256 - yaiV b0375 0.84 +0.2 394,262 + yaiV b0375 0.85 +0.0 394,264 + yaiV b0375 0.86 -1.2 394,264 + yaiV b0375 0.86 -0.4 394,271 + yaiV b0375 0.87 +0.1 394,279 - yaiV b0375 0.88 -0.4 394,281 + yaiV b0375 0.88 -0.6 394,281 + yaiV b0375 0.88 +0.6 394,313 - -0.5 394,313 - +0.7 394,325 + +0.8 394,334 - +0.8 394,335 + +0.2 394,355 - +1.1 394,355 - -1.2 394,355 - +0.9 394,355 - +0.1 394,355 - +0.2 394,357 - -0.2 394,378 - +0.3 394,378 - -0.4 394,381 + +0.8 394,389 - +0.5 394,389 - -0.3 394,394 - +0.0 394,394 - -0.4 394,394 - +0.4 394,402 - -0.5 394,408 + +0.6 394,408 + -0.8 394,413 - -0.1 394,413 - -0.1 394,416 - +0.2 394,416 - +0.6 394,416 - +0.2 394,421 - -0.3 394,421 - -0.4 394,446 - -0.2 394,446 - +0.1 394,454 - -0.0 394,454 - +0.2 394,461 - -0.2 394,467 - +0.2 394,469 - -0.0 394,469 - -0.4 394,475 + ampH b0376 0.10 +0.5 394,475 + ampH b0376 0.10 -1.4 394,497 + ampH b0376 0.12 +1.4 394,502 - ampH b0376 0.13 +0.2 394,504 + ampH b0376 0.13 +1.4 394,505 - ampH b0376 0.13 +0.1 394,505 - ampH b0376 0.13 +0.0 394,542 + ampH b0376 0.16 -1.3 394,560 - ampH b0376 0.18 +0.4 394,565 + ampH b0376 0.18 -0.9 394,576 - ampH b0376 0.19 +0.1 394,576 - ampH b0376 0.19 -0.3 394,576 - ampH b0376 0.19 -0.4 394,582 - ampH b0376 0.20 -0.1 394,616 - ampH b0376 0.23 +0.2 394,619 - ampH b0376 0.23 -0.0 394,620 - ampH b0376 0.23 +0.1 394,628 + ampH b0376 0.24 -1.1 394,665 + ampH b0376 0.27 +0.4 394,665 + ampH b0376 0.27 -0.2 394,719 - ampH b0376 0.32 +0.7 394,850 - ampH b0376 0.43 +0.5 394,850 - ampH b0376 0.43 -0.2 394,853 + ampH b0376 0.43 +0.1 394,909 - ampH b0376 0.48 -0.2 394,942 - ampH b0376 0.51 -1.6 394,942 - ampH b0376 0.51 -0.5 394,942 - ampH b0376 0.51 -0.3 394,955 + ampH b0376 0.52 -0.1 394,956 + ampH b0376 0.52 +0.0 394,964 - ampH b0376 0.53 +1.0 394,964 - ampH b0376 0.53 +0.0 395,074 - ampH b0376 0.62 +0.1 395,200 + ampH b0376 0.73 -0.5 395,200 + ampH b0376 0.73 -0.3 395,208 - ampH b0376 0.74 +0.0 395,208 - ampH b0376 0.74 +0.0 395,223 + ampH b0376 0.75 -0.4 395,227 + ampH b0376 0.75 +0.0 395,227 + ampH b0376 0.75 +0.1 395,280 - ampH b0376 0.80 +0.0 395,298 - ampH b0376 0.82 -0.2 395,350 + ampH b0376 0.86 -0.3
Or see this region's nucleotide sequence