Experiment: LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrcB and fhuA are separated by 219 nucleotides fhuA and fhuC are separated by 50 nucleotides fhuC and fhuD overlap by 1 nucleotides
b0149: mrcB - penicillin-binding protein 1b (NCBI), at 164,730 to 167,264
mrcB
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 166.520 kb on - strand, within mrcB at 166.621 kb on + strand, within mrcB at 166.621 kb on + strand, within mrcB at 166.627 kb on + strand, within mrcB at 166.627 kb on + strand, within mrcB at 166.635 kb on - strand, within mrcB at 166.672 kb on + strand, within mrcB at 166.682 kb on + strand, within mrcB at 166.682 kb on + strand, within mrcB at 166.713 kb on + strand, within mrcB at 166.732 kb on + strand, within mrcB at 166.756 kb on + strand, within mrcB at 166.756 kb on + strand, within mrcB at 166.757 kb on - strand, within mrcB at 166.774 kb on - strand, within mrcB at 166.839 kb on - strand, within mrcB at 166.848 kb on + strand, within mrcB at 166.858 kb on - strand, within mrcB at 166.898 kb on - strand, within mrcB at 166.932 kb on + strand, within mrcB at 166.934 kb on - strand, within mrcB at 166.961 kb on + strand, within mrcB at 167.081 kb on + strand at 167.095 kb on - strand at 167.103 kb on - strand at 167.103 kb on - strand at 167.124 kb on + strand at 167.127 kb on - strand at 167.127 kb on - strand at 167.135 kb on - strand at 167.135 kb on - strand at 167.210 kb on + strand at 167.210 kb on + strand at 167.214 kb on + strand at 167.214 kb on + strand at 167.232 kb on - strand at 167.244 kb on - strand at 167.244 kb on - strand at 167.258 kb on + strand at 167.463 kb on + strand at 167.546 kb on - strand at 167.568 kb on + strand at 167.626 kb on - strand at 167.626 kb on - strand at 167.734 kb on - strand, within fhuA at 167.831 kb on + strand, within fhuA at 167.831 kb on + strand, within fhuA at 167.838 kb on + strand, within fhuA at 167.838 kb on + strand, within fhuA at 167.863 kb on + strand, within fhuA at 167.863 kb on + strand, within fhuA at 167.955 kb on + strand, within fhuA at 167.963 kb on - strand, within fhuA at 168.024 kb on + strand, within fhuA at 168.024 kb on + strand, within fhuA at 168.026 kb on + strand, within fhuA at 168.034 kb on - strand, within fhuA at 168.072 kb on + strand, within fhuA at 168.072 kb on + strand, within fhuA at 168.091 kb on + strand, within fhuA at 168.138 kb on - strand, within fhuA at 168.138 kb on - strand, within fhuA at 168.177 kb on - strand, within fhuA at 168.191 kb on - strand, within fhuA at 168.191 kb on - strand, within fhuA at 168.362 kb on + strand, within fhuA at 168.362 kb on + strand, within fhuA at 168.373 kb on - strand, within fhuA at 168.390 kb on + strand, within fhuA at 168.390 kb on + strand, within fhuA at 168.395 kb on + strand, within fhuA at 168.395 kb on + strand, within fhuA at 168.406 kb on + strand, within fhuA at 168.406 kb on + strand, within fhuA at 168.436 kb on - strand, within fhuA at 168.436 kb on - strand, within fhuA at 168.439 kb on - strand, within fhuA at 168.621 kb on + strand, within fhuA at 168.621 kb on + strand, within fhuA at 168.777 kb on - strand, within fhuA at 168.800 kb on + strand, within fhuA at 168.899 kb on + strand, within fhuA at 168.942 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.961 kb on + strand, within fhuA at 169.064 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.202 kb on + strand, within fhuA at 169.260 kb on + strand, within fhuA at 169.330 kb on + strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.355 kb on - strand, within fhuA at 169.361 kb on + strand, within fhuA at 169.390 kb on - strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.467 kb on + strand, within fhuA at 169.483 kb on + strand, within fhuA at 169.490 kb on + strand, within fhuA at 169.528 kb on + strand at 169.549 kb on - strand at 169.556 kb on - strand at 169.594 kb on - strand at 169.620 kb on + strand at 169.629 kb on - strand at 169.635 kb on - strand at 169.635 kb on - strand at 169.639 kb on - strand at 169.684 kb on - strand at 169.827 kb on - strand at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.929 kb on - strand, within fhuC at 169.965 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.989 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.250 kb on + strand, within fhuC at 170.250 kb on - strand, within fhuC at 170.255 kb on + strand, within fhuC at 170.257 kb on + strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.395 kb on - strand, within fhuC at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD
Per-strain Table
Position Strand Gene LocusTag Fraction LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector remove 166,520 - mrcB b0149 0.71 +0.1 166,621 + mrcB b0149 0.75 -0.8 166,621 + mrcB b0149 0.75 -1.8 166,627 + mrcB b0149 0.75 -1.0 166,627 + mrcB b0149 0.75 -2.3 166,635 - mrcB b0149 0.75 -2.0 166,672 + mrcB b0149 0.77 -0.8 166,682 + mrcB b0149 0.77 -2.2 166,682 + mrcB b0149 0.77 -0.9 166,713 + mrcB b0149 0.78 -1.6 166,732 + mrcB b0149 0.79 -0.3 166,756 + mrcB b0149 0.80 -1.1 166,756 + mrcB b0149 0.80 -2.6 166,757 - mrcB b0149 0.80 +0.1 166,774 - mrcB b0149 0.81 -3.1 166,839 - mrcB b0149 0.83 -0.7 166,848 + mrcB b0149 0.84 -1.2 166,858 - mrcB b0149 0.84 -1.6 166,898 - mrcB b0149 0.86 -2.0 166,932 + mrcB b0149 0.87 -1.8 166,934 - mrcB b0149 0.87 -1.5 166,961 + mrcB b0149 0.88 -1.5 167,081 + +0.3 167,095 - +0.4 167,103 - -0.3 167,103 - +0.9 167,124 + +0.0 167,127 - +0.1 167,127 - -0.3 167,135 - +0.1 167,135 - +0.2 167,210 + -0.1 167,210 + +0.1 167,214 + -0.2 167,214 + +0.1 167,232 - -0.3 167,244 - -0.1 167,244 - -0.1 167,258 + +1.0 167,463 + +0.3 167,546 - +0.2 167,568 + -0.2 167,626 - -0.4 167,626 - +0.3 167,734 - fhuA b0150 0.11 -0.5 167,831 + fhuA b0150 0.15 -0.1 167,831 + fhuA b0150 0.15 +0.2 167,838 + fhuA b0150 0.16 -0.1 167,838 + fhuA b0150 0.16 +0.3 167,863 + fhuA b0150 0.17 -0.1 167,863 + fhuA b0150 0.17 -0.3 167,955 + fhuA b0150 0.21 -0.2 167,963 - fhuA b0150 0.21 +0.8 168,024 + fhuA b0150 0.24 -0.4 168,024 + fhuA b0150 0.24 +0.3 168,026 + fhuA b0150 0.24 -0.1 168,034 - fhuA b0150 0.25 +0.6 168,072 + fhuA b0150 0.26 +0.1 168,072 + fhuA b0150 0.26 -0.1 168,091 + fhuA b0150 0.27 -0.0 168,138 - fhuA b0150 0.29 +0.0 168,138 - fhuA b0150 0.29 -0.0 168,177 - fhuA b0150 0.31 +0.2 168,191 - fhuA b0150 0.32 +0.1 168,191 - fhuA b0150 0.32 -0.0 168,362 + fhuA b0150 0.39 +0.2 168,362 + fhuA b0150 0.39 -0.3 168,373 - fhuA b0150 0.40 -0.6 168,390 + fhuA b0150 0.40 +0.0 168,390 + fhuA b0150 0.40 -1.5 168,395 + fhuA b0150 0.41 +0.3 168,395 + fhuA b0150 0.41 -0.3 168,406 + fhuA b0150 0.41 -0.2 168,406 + fhuA b0150 0.41 -0.8 168,436 - fhuA b0150 0.42 -0.4 168,436 - fhuA b0150 0.42 +0.1 168,439 - fhuA b0150 0.43 -0.4 168,621 + fhuA b0150 0.51 +0.3 168,621 + fhuA b0150 0.51 -0.1 168,777 - fhuA b0150 0.58 -0.1 168,800 + fhuA b0150 0.59 +0.1 168,899 + fhuA b0150 0.63 +0.2 168,942 - fhuA b0150 0.65 -0.5 168,944 - fhuA b0150 0.65 -0.1 168,944 - fhuA b0150 0.65 -0.0 168,961 + fhuA b0150 0.66 -0.8 169,064 + fhuA b0150 0.70 -0.1 169,127 + fhuA b0150 0.73 -0.2 169,127 + fhuA b0150 0.73 +0.2 169,135 - fhuA b0150 0.74 +0.9 169,135 - fhuA b0150 0.74 +0.3 169,167 - fhuA b0150 0.75 -0.4 169,167 - fhuA b0150 0.75 -0.6 169,202 + fhuA b0150 0.77 +0.1 169,260 + fhuA b0150 0.79 -0.1 169,330 + fhuA b0150 0.82 +0.6 169,354 - fhuA b0150 0.83 -0.2 169,354 - fhuA b0150 0.83 -0.2 169,355 - fhuA b0150 0.83 +0.4 169,361 + fhuA b0150 0.84 -0.6 169,390 - fhuA b0150 0.85 +0.4 169,437 + fhuA b0150 0.87 +0.1 169,437 + fhuA b0150 0.87 -0.4 169,467 + fhuA b0150 0.88 -0.1 169,483 + fhuA b0150 0.89 -0.7 169,490 + fhuA b0150 0.89 -0.0 169,528 + -0.3 169,549 - +0.1 169,556 - +0.1 169,594 - -0.6 169,620 + -0.1 169,629 - -0.2 169,635 - -0.7 169,635 - +0.5 169,639 - +0.1 169,684 - -0.3 169,827 - -0.1 169,926 + fhuC b0151 0.19 +0.6 169,926 + fhuC b0151 0.19 +0.0 169,926 + fhuC b0151 0.19 +0.3 169,929 - fhuC b0151 0.19 +0.1 169,965 + fhuC b0151 0.23 -0.5 169,966 + fhuC b0151 0.24 -0.1 169,966 + fhuC b0151 0.24 +0.1 169,989 + fhuC b0151 0.26 +0.4 170,210 + fhuC b0151 0.54 -0.8 170,210 + fhuC b0151 0.54 +0.6 170,250 + fhuC b0151 0.59 +0.4 170,250 - fhuC b0151 0.59 +0.4 170,255 + fhuC b0151 0.60 -0.6 170,257 + fhuC b0151 0.60 -0.0 170,390 - fhuC b0151 0.77 -0.1 170,390 - fhuC b0151 0.77 -0.0 170,395 - fhuC b0151 0.77 +0.4 170,654 - -0.0 170,671 + fhuD b0152 0.11 -0.3 170,671 + fhuD b0152 0.11 -0.0 170,689 - fhuD b0152 0.13 -0.4
Or see this region's nucleotide sequence