Strain Fitness in Escherichia coli BW25113 around b2821

Experiment: Control_Tn2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecB and ptr overlap by 8 nucleotidesptr and recC are separated by 175 nucleotides b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025 recB b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906 ptr b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450 recC Position (kb) 2954 2955 2956 2957Strain fitness (log2 ratio) -2 -1 0 1at 2953.040 kb on - strand, within recBat 2953.978 kb on + strandat 2953.978 kb on + strandat 2954.006 kb on - strandat 2954.072 kb on + strandat 2954.072 kb on + strandat 2954.201 kb on - strandat 2954.359 kb on + strand, within ptrat 2954.508 kb on + strand, within ptrat 2954.516 kb on + strand, within ptrat 2954.587 kb on - strand, within ptrat 2954.617 kb on + strand, within ptrat 2954.617 kb on + strand, within ptrat 2954.626 kb on - strand, within ptrat 2954.626 kb on - strand, within ptrat 2954.713 kb on - strand, within ptrat 2954.724 kb on - strand, within ptrat 2954.759 kb on - strand, within ptrat 2954.759 kb on - strand, within ptrat 2954.771 kb on - strand, within ptrat 2954.771 kb on - strand, within ptrat 2954.777 kb on - strand, within ptrat 2954.782 kb on - strand, within ptrat 2954.782 kb on - strand, within ptrat 2954.857 kb on + strand, within ptrat 2954.857 kb on + strand, within ptrat 2954.905 kb on + strand, within ptrat 2954.905 kb on + strand, within ptrat 2954.985 kb on + strand, within ptrat 2954.985 kb on + strand, within ptrat 2955.059 kb on + strand, within ptrat 2955.080 kb on - strand, within ptrat 2955.090 kb on + strand, within ptrat 2955.232 kb on - strand, within ptrat 2955.232 kb on - strand, within ptrat 2955.232 kb on - strand, within ptrat 2955.265 kb on - strand, within ptrat 2955.334 kb on + strand, within ptrat 2955.413 kb on - strand, within ptrat 2955.413 kb on - strand, within ptrat 2955.482 kb on - strand, within ptrat 2955.573 kb on + strand, within ptrat 2955.733 kb on + strand, within ptrat 2955.842 kb on - strand, within ptrat 2955.842 kb on - strand, within ptrat 2955.842 kb on - strand, within ptrat 2955.907 kb on + strand, within ptrat 2955.907 kb on + strand, within ptrat 2955.912 kb on + strand, within ptrat 2955.940 kb on + strand, within ptrat 2956.077 kb on - strand, within ptrat 2956.077 kb on - strand, within ptrat 2956.082 kb on - strand, within ptrat 2956.082 kb on - strand, within ptrat 2956.353 kb on - strand, within ptrat 2956.360 kb on + strand, within ptrat 2956.362 kb on + strand, within ptrat 2956.370 kb on - strand, within ptrat 2956.419 kb on - strand, within ptrat 2956.478 kb on - strand, within ptrat 2956.478 kb on - strand, within ptrat 2956.485 kb on + strand, within ptrat 2956.485 kb on + strand, within ptrat 2956.598 kb on - strand, within ptrat 2956.607 kb on - strand, within ptrat 2956.653 kb on + strandat 2956.658 kb on - strandat 2956.658 kb on - strandat 2956.663 kb on + strandat 2956.772 kb on - strandat 2956.772 kb on - strandat 2956.844 kb on + strandat 2956.882 kb on - strandat 2957.218 kb on - strandat 2957.288 kb on + strandat 2957.823 kb on + strand, within recC

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_Tn2
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2,953,040 - recB b2820 0.72 +0.7
2,953,978 + +0.4
2,953,978 + -0.4
2,954,006 - -1.9
2,954,072 + +0.0
2,954,072 + -0.1
2,954,201 - +0.3
2,954,359 + ptr b2821 0.12 +0.0
2,954,508 + ptr b2821 0.17 +1.0
2,954,516 + ptr b2821 0.17 -0.0
2,954,587 - ptr b2821 0.20 -0.3
2,954,617 + ptr b2821 0.21 -0.4
2,954,617 + ptr b2821 0.21 -0.4
2,954,626 - ptr b2821 0.21 -0.8
2,954,626 - ptr b2821 0.21 -0.7
2,954,713 - ptr b2821 0.24 +0.1
2,954,724 - ptr b2821 0.24 +0.1
2,954,759 - ptr b2821 0.26 +0.0
2,954,759 - ptr b2821 0.26 +0.3
2,954,771 - ptr b2821 0.26 -1.3
2,954,771 - ptr b2821 0.26 -1.0
2,954,777 - ptr b2821 0.26 -0.4
2,954,782 - ptr b2821 0.26 -0.2
2,954,782 - ptr b2821 0.26 -0.4
2,954,857 + ptr b2821 0.29 +0.1
2,954,857 + ptr b2821 0.29 +0.4
2,954,905 + ptr b2821 0.31 -0.8
2,954,905 + ptr b2821 0.31 +0.1
2,954,985 + ptr b2821 0.33 +0.4
2,954,985 + ptr b2821 0.33 +0.0
2,955,059 + ptr b2821 0.36 -0.1
2,955,080 - ptr b2821 0.37 -0.3
2,955,090 + ptr b2821 0.37 -0.7
2,955,232 - ptr b2821 0.42 -0.2
2,955,232 - ptr b2821 0.42 +1.1
2,955,232 - ptr b2821 0.42 -0.3
2,955,265 - ptr b2821 0.43 -0.1
2,955,334 + ptr b2821 0.46 +0.5
2,955,413 - ptr b2821 0.48 -0.3
2,955,413 - ptr b2821 0.48 -0.4
2,955,482 - ptr b2821 0.51 +0.2
2,955,573 + ptr b2821 0.54 -0.1
2,955,733 + ptr b2821 0.59 -0.0
2,955,842 - ptr b2821 0.63 -0.2
2,955,842 - ptr b2821 0.63 +0.1
2,955,842 - ptr b2821 0.63 -0.5
2,955,907 + ptr b2821 0.65 +1.2
2,955,907 + ptr b2821 0.65 -0.3
2,955,912 + ptr b2821 0.66 -0.1
2,955,940 + ptr b2821 0.67 -0.0
2,956,077 - ptr b2821 0.71 -0.8
2,956,077 - ptr b2821 0.71 +0.5
2,956,082 - ptr b2821 0.71 -0.9
2,956,082 - ptr b2821 0.71 -0.2
2,956,353 - ptr b2821 0.81 +0.8
2,956,360 + ptr b2821 0.81 +0.2
2,956,362 + ptr b2821 0.81 -0.9
2,956,370 - ptr b2821 0.81 -0.1
2,956,419 - ptr b2821 0.83 -0.2
2,956,478 - ptr b2821 0.85 +0.3
2,956,478 - ptr b2821 0.85 -0.1
2,956,485 + ptr b2821 0.85 -0.2
2,956,485 + ptr b2821 0.85 -0.5
2,956,598 - ptr b2821 0.89 -0.4
2,956,607 - ptr b2821 0.90 -0.1
2,956,653 + +0.5
2,956,658 - -0.1
2,956,658 - +0.5
2,956,663 + -0.0
2,956,772 - -0.5
2,956,772 - +0.5
2,956,844 + -0.9
2,956,882 - +0.1
2,957,218 - +0.7
2,957,288 + +0.1
2,957,823 + recC b2822 0.22 +0.7

Or see this region's nucleotide sequence