Experiment: Control_Tn1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malT and rtcA are separated by 42 nucleotides rtcA and rtcB are separated by 3 nucleotides
b3418: malT - transcriptional regulator MalT (NCBI), at 3,551,107 to 3,553,812
malT
b4475: rtcA - RNA 3'-terminal-phosphate cyclase (NCBI), at 3,553,855 to 3,554,871
rtcA
b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101
rtcB
Position (kb)
3553
3554
3555 Strain fitness (log2 ratio)
-1
0
1
2 at 3552.885 kb on + strand, within malT at 3552.936 kb on - strand, within malT at 3552.942 kb on + strand, within malT at 3552.942 kb on + strand, within malT at 3553.017 kb on + strand, within malT at 3553.119 kb on - strand, within malT at 3553.119 kb on - strand, within malT at 3553.142 kb on - strand, within malT at 3553.179 kb on + strand, within malT at 3553.258 kb on - strand, within malT at 3553.309 kb on + strand, within malT at 3553.317 kb on + strand, within malT at 3553.351 kb on + strand, within malT at 3553.351 kb on + strand, within malT at 3553.365 kb on - strand, within malT at 3553.411 kb on + strand, within malT at 3553.411 kb on + strand, within malT at 3553.480 kb on - strand, within malT at 3553.493 kb on + strand, within malT at 3553.493 kb on + strand, within malT at 3553.622 kb on + strand at 3553.622 kb on + strand at 3553.628 kb on + strand at 3553.628 kb on + strand at 3553.628 kb on + strand at 3553.628 kb on + strand at 3553.654 kb on + strand at 3553.654 kb on + strand at 3553.654 kb on + strand at 3553.657 kb on - strand at 3553.657 kb on - strand at 3553.662 kb on - strand at 3553.662 kb on - strand at 3553.765 kb on + strand at 3553.834 kb on + strand at 3553.848 kb on - strand at 3553.857 kb on + strand at 3553.880 kb on - strand at 3553.880 kb on - strand at 3553.919 kb on + strand at 3553.934 kb on + strand at 3553.942 kb on - strand at 3553.951 kb on + strand at 3553.964 kb on - strand, within rtcA at 3553.966 kb on - strand, within rtcA at 3554.026 kb on - strand, within rtcA at 3554.026 kb on - strand, within rtcA at 3554.035 kb on - strand, within rtcA at 3554.035 kb on - strand, within rtcA at 3554.075 kb on - strand, within rtcA at 3554.077 kb on - strand, within rtcA at 3554.091 kb on + strand, within rtcA at 3554.149 kb on - strand, within rtcA at 3554.168 kb on + strand, within rtcA at 3554.168 kb on + strand, within rtcA at 3554.176 kb on - strand, within rtcA at 3554.178 kb on + strand, within rtcA at 3554.178 kb on + strand, within rtcA at 3554.204 kb on - strand, within rtcA at 3554.228 kb on - strand, within rtcA at 3554.248 kb on + strand, within rtcA at 3554.256 kb on + strand, within rtcA at 3554.312 kb on + strand, within rtcA at 3554.312 kb on + strand, within rtcA at 3554.336 kb on + strand, within rtcA at 3554.338 kb on + strand, within rtcA at 3554.378 kb on - strand, within rtcA at 3554.457 kb on + strand, within rtcA at 3554.457 kb on + strand, within rtcA at 3554.497 kb on + strand, within rtcA at 3554.528 kb on + strand, within rtcA at 3554.530 kb on + strand, within rtcA at 3554.534 kb on - strand, within rtcA at 3554.534 kb on - strand, within rtcA at 3554.656 kb on + strand, within rtcA at 3554.664 kb on - strand, within rtcA at 3554.664 kb on - strand, within rtcA at 3554.671 kb on + strand, within rtcA at 3554.679 kb on - strand, within rtcA at 3554.679 kb on - strand, within rtcA at 3554.679 kb on - strand, within rtcA at 3554.679 kb on - strand, within rtcA at 3554.722 kb on - strand, within rtcA at 3554.722 kb on - strand, within rtcA at 3554.767 kb on - strand, within rtcA at 3554.767 kb on - strand, within rtcA at 3554.770 kb on + strand at 3554.779 kb on - strand at 3554.800 kb on + strand at 3554.800 kb on + strand at 3554.869 kb on - strand at 3554.904 kb on + strand at 3554.904 kb on + strand at 3554.904 kb on + strand at 3554.904 kb on + strand at 3554.906 kb on + strand at 3554.912 kb on - strand at 3554.912 kb on - strand at 3554.912 kb on - strand at 3554.912 kb on - strand at 3554.912 kb on - strand at 3554.914 kb on - strand at 3554.914 kb on - strand at 3555.004 kb on - strand, within rtcB at 3555.081 kb on - strand, within rtcB at 3555.175 kb on + strand, within rtcB at 3555.196 kb on - strand, within rtcB at 3555.198 kb on - strand, within rtcB at 3555.215 kb on + strand, within rtcB at 3555.243 kb on - strand, within rtcB at 3555.243 kb on - strand, within rtcB at 3555.284 kb on - strand, within rtcB at 3555.394 kb on - strand, within rtcB at 3555.398 kb on + strand, within rtcB at 3555.428 kb on + strand, within rtcB at 3555.428 kb on - strand, within rtcB at 3555.428 kb on - strand, within rtcB at 3555.437 kb on + strand, within rtcB at 3555.447 kb on - strand, within rtcB at 3555.465 kb on + strand, within rtcB at 3555.535 kb on + strand, within rtcB at 3555.552 kb on + strand, within rtcB at 3555.554 kb on + strand, within rtcB at 3555.565 kb on - strand, within rtcB at 3555.565 kb on - strand, within rtcB at 3555.581 kb on + strand, within rtcB at 3555.591 kb on + strand, within rtcB at 3555.591 kb on + strand, within rtcB at 3555.714 kb on + strand, within rtcB at 3555.720 kb on - strand, within rtcB at 3555.749 kb on + strand, within rtcB at 3555.749 kb on + strand, within rtcB at 3555.826 kb on - strand, within rtcB at 3555.826 kb on - strand, within rtcB
Per-strain Table
Position Strand Gene LocusTag Fraction Control_Tn1 remove 3,552,885 + malT b3418 0.66 -0.1 3,552,936 - malT b3418 0.68 -1.0 3,552,942 + malT b3418 0.68 +0.5 3,552,942 + malT b3418 0.68 +0.6 3,553,017 + malT b3418 0.71 -0.2 3,553,119 - malT b3418 0.74 -0.7 3,553,119 - malT b3418 0.74 +0.4 3,553,142 - malT b3418 0.75 +0.3 3,553,179 + malT b3418 0.77 +0.3 3,553,258 - malT b3418 0.79 -1.3 3,553,309 + malT b3418 0.81 -0.3 3,553,317 + malT b3418 0.82 +1.0 3,553,351 + malT b3418 0.83 -0.2 3,553,351 + malT b3418 0.83 -0.2 3,553,365 - malT b3418 0.83 +0.4 3,553,411 + malT b3418 0.85 +0.7 3,553,411 + malT b3418 0.85 -0.1 3,553,480 - malT b3418 0.88 +0.6 3,553,493 + malT b3418 0.88 -0.3 3,553,493 + malT b3418 0.88 -0.7 3,553,622 + +0.3 3,553,622 + +0.6 3,553,628 + +0.8 3,553,628 + -0.1 3,553,628 + +0.2 3,553,628 + +0.6 3,553,654 + -0.5 3,553,654 + -0.1 3,553,654 + -0.7 3,553,657 - -0.6 3,553,657 - +0.1 3,553,662 - +1.8 3,553,662 - +0.4 3,553,765 + -1.6 3,553,834 + +0.1 3,553,848 - +0.1 3,553,857 + +0.2 3,553,880 - +1.5 3,553,880 - +0.5 3,553,919 + +0.1 3,553,934 + +0.4 3,553,942 - -0.0 3,553,951 + -0.1 3,553,964 - rtcA b4475 0.11 -0.8 3,553,966 - rtcA b4475 0.11 +1.0 3,554,026 - rtcA b4475 0.17 -0.5 3,554,026 - rtcA b4475 0.17 -0.9 3,554,035 - rtcA b4475 0.18 +0.6 3,554,035 - rtcA b4475 0.18 +0.7 3,554,075 - rtcA b4475 0.22 -0.1 3,554,077 - rtcA b4475 0.22 +0.7 3,554,091 + rtcA b4475 0.23 -0.3 3,554,149 - rtcA b4475 0.29 -0.1 3,554,168 + rtcA b4475 0.31 -0.6 3,554,168 + rtcA b4475 0.31 +0.4 3,554,176 - rtcA b4475 0.32 -0.5 3,554,178 + rtcA b4475 0.32 +0.7 3,554,178 + rtcA b4475 0.32 -0.4 3,554,204 - rtcA b4475 0.34 -0.1 3,554,228 - rtcA b4475 0.37 -0.7 3,554,248 + rtcA b4475 0.39 -0.7 3,554,256 + rtcA b4475 0.39 -0.9 3,554,312 + rtcA b4475 0.45 -0.2 3,554,312 + rtcA b4475 0.45 -0.3 3,554,336 + rtcA b4475 0.47 -0.7 3,554,338 + rtcA b4475 0.47 -0.9 3,554,378 - rtcA b4475 0.51 -0.1 3,554,457 + rtcA b4475 0.59 -0.3 3,554,457 + rtcA b4475 0.59 -0.5 3,554,497 + rtcA b4475 0.63 -0.4 3,554,528 + rtcA b4475 0.66 -0.0 3,554,530 + rtcA b4475 0.66 -0.1 3,554,534 - rtcA b4475 0.67 +0.5 3,554,534 - rtcA b4475 0.67 -0.3 3,554,656 + rtcA b4475 0.79 +0.6 3,554,664 - rtcA b4475 0.80 +0.0 3,554,664 - rtcA b4475 0.80 +2.1 3,554,671 + rtcA b4475 0.80 -0.6 3,554,679 - rtcA b4475 0.81 +0.0 3,554,679 - rtcA b4475 0.81 -0.0 3,554,679 - rtcA b4475 0.81 +1.0 3,554,679 - rtcA b4475 0.81 +0.1 3,554,722 - rtcA b4475 0.85 -0.1 3,554,722 - rtcA b4475 0.85 -0.1 3,554,767 - rtcA b4475 0.90 +2.1 3,554,767 - rtcA b4475 0.90 -1.0 3,554,770 + -0.5 3,554,779 - -0.3 3,554,800 + -0.2 3,554,800 + +0.1 3,554,869 - -0.1 3,554,904 + +0.1 3,554,904 + -0.4 3,554,904 + +0.3 3,554,904 + -0.1 3,554,906 + -0.4 3,554,912 - -0.1 3,554,912 - +0.7 3,554,912 - -0.5 3,554,912 - -1.1 3,554,912 - -0.6 3,554,914 - +1.4 3,554,914 - -0.9 3,555,004 - rtcB b3421 0.11 -0.0 3,555,081 - rtcB b3421 0.17 -0.2 3,555,175 + rtcB b3421 0.24 -0.1 3,555,196 - rtcB b3421 0.26 -0.2 3,555,198 - rtcB b3421 0.26 -0.6 3,555,215 + rtcB b3421 0.28 -1.3 3,555,243 - rtcB b3421 0.30 -0.9 3,555,243 - rtcB b3421 0.30 +0.2 3,555,284 - rtcB b3421 0.33 +0.9 3,555,394 - rtcB b3421 0.42 +1.0 3,555,398 + rtcB b3421 0.43 -0.0 3,555,428 + rtcB b3421 0.45 +0.8 3,555,428 - rtcB b3421 0.45 +0.9 3,555,428 - rtcB b3421 0.45 +0.9 3,555,437 + rtcB b3421 0.46 +0.8 3,555,447 - rtcB b3421 0.47 -0.6 3,555,465 + rtcB b3421 0.48 -1.1 3,555,535 + rtcB b3421 0.54 -0.3 3,555,552 + rtcB b3421 0.55 -0.2 3,555,554 + rtcB b3421 0.55 +0.2 3,555,565 - rtcB b3421 0.56 -0.6 3,555,565 - rtcB b3421 0.56 +0.5 3,555,581 + rtcB b3421 0.58 +0.3 3,555,591 + rtcB b3421 0.58 -0.3 3,555,591 + rtcB b3421 0.58 +0.1 3,555,714 + rtcB b3421 0.68 +1.7 3,555,720 - rtcB b3421 0.69 +0.6 3,555,749 + rtcB b3421 0.71 -0.2 3,555,749 + rtcB b3421 0.71 -0.3 3,555,826 - rtcB b3421 0.78 +0.1 3,555,826 - rtcB b3421 0.78 +0.8
Or see this region's nucleotide sequence