Experiment: Laval1374
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrD and yidF are separated by 7 nucleotides yidF and yidG overlap by 4 nucleotides yidG and yidH overlap by 11 nucleotides yidH and yidI are separated by 107 nucleotides yidI and yidJ are separated by 46 nucleotides
b3673: emrD - 2-module integral membrane pump; multidrug resistance (VIMSS), at 3,851,945 to 3,853,129
emrD
b3674: yidF - predicted DNA-binding transcriptional regulator (NCBI), at 3,853,137 to 3,853,634
yidF
b3675: yidG - predicted inner membrane protein (NCBI), at 3,853,631 to 3,853,993
yidG
b3676: yidH - conserved inner membrane protein (NCBI), at 3,853,983 to 3,854,330
yidH
b3677: yidI - predicted inner membrane protein (NCBI), at 3,854,438 to 3,854,887
yidI
b3678: yidJ - predicted sulfatase/phosphatase (NCBI), at 3,854,934 to 3,856,427
yidJ
Position (kb)
3853
3854
3855 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3853.003 kb on - strand, within emrD at 3853.023 kb on - strand at 3853.025 kb on + strand at 3853.035 kb on + strand at 3853.035 kb on + strand at 3853.042 kb on - strand at 3853.043 kb on - strand at 3853.043 kb on - strand at 3853.078 kb on + strand at 3853.078 kb on + strand at 3853.078 kb on + strand at 3853.086 kb on - strand at 3853.114 kb on + strand at 3853.133 kb on + strand at 3853.147 kb on + strand at 3853.147 kb on + strand at 3853.158 kb on - strand at 3853.160 kb on + strand at 3853.160 kb on - strand at 3853.160 kb on - strand at 3853.168 kb on - strand at 3853.178 kb on + strand at 3853.178 kb on + strand at 3853.216 kb on - strand, within yidF at 3853.216 kb on - strand, within yidF at 3853.233 kb on + strand, within yidF at 3853.238 kb on + strand, within yidF at 3853.257 kb on + strand, within yidF at 3853.257 kb on + strand, within yidF at 3853.263 kb on - strand, within yidF at 3853.265 kb on - strand, within yidF at 3853.284 kb on - strand, within yidF at 3853.295 kb on - strand, within yidF at 3853.295 kb on - strand, within yidF at 3853.378 kb on + strand, within yidF at 3853.390 kb on - strand, within yidF at 3853.390 kb on - strand, within yidF at 3853.393 kb on + strand, within yidF at 3853.399 kb on + strand, within yidF at 3853.399 kb on + strand, within yidF at 3853.407 kb on - strand, within yidF at 3853.418 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.419 kb on - strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.445 kb on + strand, within yidF at 3853.450 kb on + strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.453 kb on - strand, within yidF at 3853.501 kb on + strand, within yidF at 3853.501 kb on + strand, within yidF at 3853.506 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.511 kb on + strand, within yidF at 3853.597 kb on - strand at 3853.597 kb on - strand at 3853.612 kb on + strand at 3853.612 kb on + strand at 3853.666 kb on + strand at 3853.687 kb on + strand, within yidG at 3853.695 kb on + strand, within yidG at 3853.697 kb on + strand, within yidG at 3853.703 kb on - strand, within yidG at 3853.727 kb on + strand, within yidG at 3853.727 kb on + strand, within yidG at 3853.781 kb on - strand, within yidG at 3853.781 kb on - strand, within yidG at 3853.796 kb on - strand, within yidG at 3853.796 kb on - strand, within yidG at 3853.819 kb on - strand, within yidG at 3853.869 kb on - strand, within yidG at 3853.904 kb on + strand, within yidG at 3853.904 kb on + strand, within yidG at 3853.914 kb on + strand, within yidG at 3853.919 kb on + strand, within yidG at 3853.933 kb on - strand, within yidG at 3853.933 kb on - strand, within yidG at 3853.959 kb on - strand at 3853.959 kb on - strand at 3853.993 kb on + strand at 3854.005 kb on - strand at 3854.011 kb on - strand at 3854.011 kb on - strand at 3854.053 kb on - strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.062 kb on + strand, within yidH at 3854.080 kb on + strand, within yidH at 3854.080 kb on + strand, within yidH at 3854.129 kb on - strand, within yidH at 3854.214 kb on - strand, within yidH at 3854.214 kb on - strand, within yidH at 3854.251 kb on - strand, within yidH at 3854.251 kb on - strand, within yidH at 3854.305 kb on + strand at 3854.356 kb on + strand at 3854.357 kb on + strand at 3854.357 kb on + strand at 3854.445 kb on + strand at 3854.445 kb on - strand at 3854.448 kb on - strand at 3854.448 kb on - strand at 3854.450 kb on - strand at 3854.562 kb on + strand, within yidI at 3854.562 kb on + strand, within yidI at 3854.575 kb on - strand, within yidI at 3854.595 kb on - strand, within yidI at 3854.693 kb on - strand, within yidI at 3854.705 kb on - strand, within yidI at 3854.705 kb on - strand, within yidI at 3854.708 kb on - strand, within yidI at 3854.743 kb on - strand, within yidI at 3854.770 kb on + strand, within yidI at 3854.803 kb on - strand, within yidI at 3854.803 kb on - strand, within yidI at 3854.889 kb on - strand at 3854.889 kb on - strand at 3854.908 kb on - strand at 3854.932 kb on + strand at 3854.997 kb on + strand at 3855.018 kb on + strand at 3855.018 kb on + strand at 3855.049 kb on - strand at 3855.053 kb on - strand at 3855.053 kb on - strand at 3855.064 kb on - strand at 3855.064 kb on - strand at 3855.072 kb on - strand at 3855.072 kb on - strand at 3855.132 kb on - strand, within yidJ at 3855.265 kb on - strand, within yidJ at 3855.265 kb on - strand, within yidJ at 3855.307 kb on - strand, within yidJ at 3855.327 kb on - strand, within yidJ
Per-strain Table
Position Strand Gene LocusTag Fraction Laval1374 remove 3,853,003 - emrD b3673 0.89 +0.6 3,853,023 - +0.1 3,853,025 + -0.1 3,853,035 + -1.7 3,853,035 + -0.3 3,853,042 - -0.2 3,853,043 - -0.6 3,853,043 - -1.4 3,853,078 + -0.1 3,853,078 + -0.0 3,853,078 + -0.1 3,853,086 - +0.2 3,853,114 + +1.0 3,853,133 + +0.2 3,853,147 + -0.4 3,853,147 + -0.3 3,853,158 - -0.1 3,853,160 + -0.0 3,853,160 - +0.4 3,853,160 - +1.6 3,853,168 - -0.6 3,853,178 + +0.2 3,853,178 + +1.3 3,853,216 - yidF b3674 0.16 -0.1 3,853,216 - yidF b3674 0.16 -0.2 3,853,233 + yidF b3674 0.19 -0.5 3,853,238 + yidF b3674 0.20 -0.6 3,853,257 + yidF b3674 0.24 -0.2 3,853,257 + yidF b3674 0.24 -0.6 3,853,263 - yidF b3674 0.25 -0.1 3,853,265 - yidF b3674 0.26 -0.1 3,853,284 - yidF b3674 0.30 +0.6 3,853,295 - yidF b3674 0.32 -0.4 3,853,295 - yidF b3674 0.32 -0.1 3,853,378 + yidF b3674 0.48 -0.6 3,853,390 - yidF b3674 0.51 +0.0 3,853,390 - yidF b3674 0.51 -1.0 3,853,393 + yidF b3674 0.51 -0.0 3,853,399 + yidF b3674 0.53 -1.0 3,853,399 + yidF b3674 0.53 -0.6 3,853,407 - yidF b3674 0.54 -1.2 3,853,418 - yidF b3674 0.56 -0.7 3,853,419 - yidF b3674 0.57 -0.4 3,853,419 - yidF b3674 0.57 -0.0 3,853,419 - yidF b3674 0.57 -2.4 3,853,419 - yidF b3674 0.57 +0.1 3,853,445 + yidF b3674 0.62 -0.2 3,853,445 + yidF b3674 0.62 -0.3 3,853,445 + yidF b3674 0.62 -0.8 3,853,450 + yidF b3674 0.63 +0.5 3,853,453 - yidF b3674 0.63 -0.1 3,853,453 - yidF b3674 0.63 +1.3 3,853,453 - yidF b3674 0.63 -1.1 3,853,501 + yidF b3674 0.73 +0.2 3,853,501 + yidF b3674 0.73 -0.4 3,853,506 + yidF b3674 0.74 -0.2 3,853,511 + yidF b3674 0.75 +0.8 3,853,511 + yidF b3674 0.75 -0.0 3,853,511 + yidF b3674 0.75 -0.6 3,853,597 - +0.4 3,853,597 - -0.6 3,853,612 + -0.5 3,853,612 + -0.4 3,853,666 + +0.1 3,853,687 + yidG b3675 0.15 +0.4 3,853,695 + yidG b3675 0.18 +0.1 3,853,697 + yidG b3675 0.18 +0.7 3,853,703 - yidG b3675 0.20 +0.1 3,853,727 + yidG b3675 0.26 -0.2 3,853,727 + yidG b3675 0.26 +0.3 3,853,781 - yidG b3675 0.41 -0.7 3,853,781 - yidG b3675 0.41 -1.4 3,853,796 - yidG b3675 0.45 +0.1 3,853,796 - yidG b3675 0.45 -1.7 3,853,819 - yidG b3675 0.52 +1.6 3,853,869 - yidG b3675 0.66 -0.0 3,853,904 + yidG b3675 0.75 -0.5 3,853,904 + yidG b3675 0.75 +0.3 3,853,914 + yidG b3675 0.78 +0.1 3,853,919 + yidG b3675 0.79 -0.2 3,853,933 - yidG b3675 0.83 +1.6 3,853,933 - yidG b3675 0.83 -1.0 3,853,959 - -0.7 3,853,959 - +0.1 3,853,993 + +0.2 3,854,005 - +0.0 3,854,011 - +0.0 3,854,011 - -0.4 3,854,053 - yidH b3676 0.20 +0.8 3,854,062 + yidH b3676 0.23 -0.7 3,854,062 + yidH b3676 0.23 +0.5 3,854,062 + yidH b3676 0.23 +0.4 3,854,080 + yidH b3676 0.28 +0.2 3,854,080 + yidH b3676 0.28 +0.0 3,854,129 - yidH b3676 0.42 +0.9 3,854,214 - yidH b3676 0.66 -0.7 3,854,214 - yidH b3676 0.66 -0.8 3,854,251 - yidH b3676 0.77 -0.5 3,854,251 - yidH b3676 0.77 +0.1 3,854,305 + +0.1 3,854,356 + +0.4 3,854,357 + -0.3 3,854,357 + -0.7 3,854,445 + +0.4 3,854,445 - +0.3 3,854,448 - -1.1 3,854,448 - -0.6 3,854,450 - -0.3 3,854,562 + yidI b3677 0.28 +0.1 3,854,562 + yidI b3677 0.28 +0.4 3,854,575 - yidI b3677 0.30 -0.1 3,854,595 - yidI b3677 0.35 +0.0 3,854,693 - yidI b3677 0.57 -0.0 3,854,705 - yidI b3677 0.59 +0.7 3,854,705 - yidI b3677 0.59 +0.3 3,854,708 - yidI b3677 0.60 -0.1 3,854,743 - yidI b3677 0.68 +2.1 3,854,770 + yidI b3677 0.74 -0.2 3,854,803 - yidI b3677 0.81 +0.8 3,854,803 - yidI b3677 0.81 +0.2 3,854,889 - +0.9 3,854,889 - +1.1 3,854,908 - -0.3 3,854,932 + +0.7 3,854,997 + +0.1 3,855,018 + -0.2 3,855,018 + -0.4 3,855,049 - -0.3 3,855,053 - -0.7 3,855,053 - +0.1 3,855,064 - -0.7 3,855,064 - -1.1 3,855,072 - +0.9 3,855,072 - -0.1 3,855,132 - yidJ b3678 0.13 +0.4 3,855,265 - yidJ b3678 0.22 -0.2 3,855,265 - yidJ b3678 0.22 +0.4 3,855,307 - yidJ b3678 0.25 -0.5 3,855,327 - yidJ b3678 0.26 -0.0
Or see this region's nucleotide sequence