Experiment: Laval1374
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsD and rcsB are separated by 16 nucleotides rcsB and rcsC are separated by 199 nucleotides
b2216: rcsD - phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI), at 2,311,510 to 2,314,182
rcsD
b2217: rcsB - DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI), at 2,314,199 to 2,314,849
rcsB
b2218: rcsC - hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq), at 2,315,049 to 2,317,898
rcsC
Position (kb)
2314
2315 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2313.222 kb on - strand, within rcsD at 2313.297 kb on + strand, within rcsD at 2313.307 kb on + strand, within rcsD at 2313.338 kb on + strand, within rcsD at 2313.349 kb on + strand, within rcsD at 2313.349 kb on + strand, within rcsD at 2313.349 kb on - strand, within rcsD at 2313.422 kb on - strand, within rcsD at 2313.518 kb on + strand, within rcsD at 2313.521 kb on - strand, within rcsD at 2313.521 kb on - strand, within rcsD at 2313.525 kb on + strand, within rcsD at 2313.525 kb on + strand, within rcsD at 2313.590 kb on - strand, within rcsD at 2313.598 kb on + strand, within rcsD at 2313.671 kb on - strand, within rcsD at 2313.741 kb on - strand, within rcsD at 2313.750 kb on - strand, within rcsD at 2313.807 kb on + strand, within rcsD at 2313.959 kb on + strand at 2314.038 kb on + strand at 2314.038 kb on - strand at 2314.102 kb on + strand at 2314.202 kb on - strand at 2314.311 kb on + strand, within rcsB at 2314.319 kb on + strand, within rcsB at 2314.323 kb on + strand, within rcsB at 2314.362 kb on - strand, within rcsB at 2314.362 kb on - strand, within rcsB at 2314.439 kb on - strand, within rcsB at 2314.439 kb on - strand, within rcsB at 2314.490 kb on + strand, within rcsB at 2314.490 kb on + strand, within rcsB at 2314.565 kb on + strand, within rcsB at 2314.599 kb on + strand, within rcsB at 2314.773 kb on + strand, within rcsB at 2314.812 kb on - strand at 2315.032 kb on - strand at 2315.045 kb on - strand at 2315.139 kb on + strand at 2315.341 kb on + strand, within rcsC at 2315.548 kb on + strand, within rcsC at 2315.559 kb on - strand, within rcsC at 2315.678 kb on + strand, within rcsC at 2315.702 kb on + strand, within rcsC at 2315.745 kb on + strand, within rcsC at 2315.782 kb on + strand, within rcsC at 2315.785 kb on - strand, within rcsC at 2315.785 kb on - strand, within rcsC
Per-strain Table
Position Strand Gene LocusTag Fraction Laval1374 remove 2,313,222 - rcsD b2216 0.64 -4.1 2,313,297 + rcsD b2216 0.67 -2.0 2,313,307 + rcsD b2216 0.67 -3.0 2,313,338 + rcsD b2216 0.68 -2.6 2,313,349 + rcsD b2216 0.69 -3.1 2,313,349 + rcsD b2216 0.69 -2.9 2,313,349 - rcsD b2216 0.69 -3.7 2,313,422 - rcsD b2216 0.72 -5.2 2,313,518 + rcsD b2216 0.75 -2.7 2,313,521 - rcsD b2216 0.75 -3.6 2,313,521 - rcsD b2216 0.75 -3.3 2,313,525 + rcsD b2216 0.75 +0.4 2,313,525 + rcsD b2216 0.75 -0.1 2,313,590 - rcsD b2216 0.78 -3.1 2,313,598 + rcsD b2216 0.78 -1.8 2,313,671 - rcsD b2216 0.81 -3.3 2,313,741 - rcsD b2216 0.83 -5.4 2,313,750 - rcsD b2216 0.84 -3.1 2,313,807 + rcsD b2216 0.86 -2.1 2,313,959 + -4.0 2,314,038 + -2.6 2,314,038 - -3.1 2,314,102 + -2.5 2,314,202 - -2.8 2,314,311 + rcsB b2217 0.17 -3.8 2,314,319 + rcsB b2217 0.18 -5.5 2,314,323 + rcsB b2217 0.19 -4.2 2,314,362 - rcsB b2217 0.25 -3.9 2,314,362 - rcsB b2217 0.25 -2.5 2,314,439 - rcsB b2217 0.37 -3.8 2,314,439 - rcsB b2217 0.37 -3.7 2,314,490 + rcsB b2217 0.45 -4.1 2,314,490 + rcsB b2217 0.45 -3.5 2,314,565 + rcsB b2217 0.56 -2.8 2,314,599 + rcsB b2217 0.61 -3.3 2,314,773 + rcsB b2217 0.88 -4.3 2,314,812 - -2.6 2,315,032 - +0.1 2,315,045 - +0.4 2,315,139 + -2.0 2,315,341 + rcsC b2218 0.10 -3.6 2,315,548 + rcsC b2218 0.18 -3.5 2,315,559 - rcsC b2218 0.18 -2.4 2,315,678 + rcsC b2218 0.22 -2.2 2,315,702 + rcsC b2218 0.23 -3.2 2,315,745 + rcsC b2218 0.24 -3.7 2,315,782 + rcsC b2218 0.26 -0.9 2,315,785 - rcsC b2218 0.26 -4.4 2,315,785 - rcsC b2218 0.26 -2.5
Or see this region's nucleotide sequence