Strain Fitness in Escherichia coli BW25113 around b2056

Experiment: Laval1374

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaF and wcaE are separated by 15 nucleotideswcaE and wcaD are separated by 10 nucleotideswcaD and wcaC overlap by 26 nucleotides b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912 wcaF b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674 wcaE b2056: wcaD - predicted colanic acid polymerase (NCBI), at 2,127,685 to 2,128,902 wcaD b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094 wcaC Position (kb) 2127 2128 2129Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2126.914 kb on + strandat 2127.243 kb on - strand, within wcaEat 2127.467 kb on - strand, within wcaEat 2127.583 kb on + strand, within wcaEat 2127.584 kb on + strand, within wcaEat 2127.617 kb on + strandat 2127.663 kb on + strandat 2127.663 kb on + strandat 2128.019 kb on - strand, within wcaDat 2128.137 kb on - strand, within wcaDat 2128.137 kb on - strand, within wcaDat 2128.146 kb on - strand, within wcaDat 2128.222 kb on - strand, within wcaDat 2128.251 kb on - strand, within wcaDat 2128.259 kb on - strand, within wcaDat 2128.259 kb on - strand, within wcaDat 2128.280 kb on - strand, within wcaDat 2128.280 kb on - strand, within wcaDat 2128.280 kb on - strand, within wcaDat 2128.299 kb on - strand, within wcaDat 2128.304 kb on - strand, within wcaDat 2128.309 kb on - strand, within wcaDat 2128.324 kb on - strand, within wcaDat 2128.324 kb on - strand, within wcaDat 2128.355 kb on - strand, within wcaDat 2128.439 kb on - strand, within wcaDat 2128.439 kb on - strand, within wcaDat 2128.475 kb on - strand, within wcaDat 2128.527 kb on + strand, within wcaDat 2128.527 kb on + strand, within wcaDat 2128.530 kb on - strand, within wcaDat 2128.530 kb on - strand, within wcaDat 2128.531 kb on - strand, within wcaDat 2128.531 kb on - strand, within wcaDat 2128.531 kb on - strand, within wcaDat 2128.531 kb on - strand, within wcaDat 2128.535 kb on - strand, within wcaDat 2128.535 kb on - strand, within wcaDat 2128.545 kb on + strand, within wcaDat 2128.604 kb on + strand, within wcaDat 2128.604 kb on + strand, within wcaDat 2128.604 kb on + strand, within wcaDat 2128.604 kb on + strand, within wcaDat 2128.612 kb on - strand, within wcaDat 2128.612 kb on - strand, within wcaDat 2128.612 kb on - strand, within wcaDat 2128.614 kb on + strand, within wcaDat 2128.617 kb on + strand, within wcaDat 2128.667 kb on - strand, within wcaDat 2128.668 kb on - strand, within wcaDat 2128.690 kb on - strand, within wcaDat 2128.696 kb on + strand, within wcaDat 2128.696 kb on + strand, within wcaDat 2128.696 kb on + strand, within wcaDat 2128.702 kb on - strand, within wcaDat 2128.704 kb on - strand, within wcaDat 2128.713 kb on - strand, within wcaDat 2128.722 kb on + strand, within wcaDat 2128.918 kb on - strandat 2128.918 kb on - strandat 2128.925 kb on + strandat 2128.957 kb on + strandat 2128.968 kb on + strandat 2129.087 kb on - strand, within wcaCat 2129.249 kb on - strand, within wcaCat 2129.249 kb on - strand, within wcaCat 2129.459 kb on + strand, within wcaCat 2129.459 kb on + strand, within wcaCat 2129.468 kb on + strand, within wcaCat 2129.473 kb on + strand, within wcaCat 2129.586 kb on + strand, within wcaCat 2129.690 kb on + strand, within wcaCat 2129.798 kb on + strand, within wcaCat 2129.803 kb on + strand, within wcaC

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1374
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2,126,914 + +1.4
2,127,243 - wcaE b2055 0.42 +1.4
2,127,467 - wcaE b2055 0.72 +1.4
2,127,583 + wcaE b2055 0.88 +1.4
2,127,584 + wcaE b2055 0.88 +1.4
2,127,617 + +1.4
2,127,663 + +1.4
2,127,663 + +1.4
2,128,019 - wcaD b2056 0.27 +1.4
2,128,137 - wcaD b2056 0.37 +0.4
2,128,137 - wcaD b2056 0.37 -0.2
2,128,146 - wcaD b2056 0.38 +1.4
2,128,222 - wcaD b2056 0.44 -1.2
2,128,251 - wcaD b2056 0.46 -1.4
2,128,259 - wcaD b2056 0.47 +0.4
2,128,259 - wcaD b2056 0.47 -0.2
2,128,280 - wcaD b2056 0.49 -0.9
2,128,280 - wcaD b2056 0.49 +0.4
2,128,280 - wcaD b2056 0.49 -0.6
2,128,299 - wcaD b2056 0.50 -1.4
2,128,304 - wcaD b2056 0.51 -1.9
2,128,309 - wcaD b2056 0.51 -3.7
2,128,324 - wcaD b2056 0.52 -2.6
2,128,324 - wcaD b2056 0.52 -1.6
2,128,355 - wcaD b2056 0.55 -1.2
2,128,439 - wcaD b2056 0.62 -0.9
2,128,439 - wcaD b2056 0.62 -3.4
2,128,475 - wcaD b2056 0.65 -3.3
2,128,527 + wcaD b2056 0.69 +0.4
2,128,527 + wcaD b2056 0.69 +0.4
2,128,530 - wcaD b2056 0.69 -4.1
2,128,530 - wcaD b2056 0.69 -3.5
2,128,531 - wcaD b2056 0.69 -2.2
2,128,531 - wcaD b2056 0.69 -3.3
2,128,531 - wcaD b2056 0.69 -2.0
2,128,531 - wcaD b2056 0.69 -3.3
2,128,535 - wcaD b2056 0.70 -1.2
2,128,535 - wcaD b2056 0.70 -2.9
2,128,545 + wcaD b2056 0.71 +1.4
2,128,604 + wcaD b2056 0.75 -0.6
2,128,604 + wcaD b2056 0.75 -0.6
2,128,604 + wcaD b2056 0.75 -0.2
2,128,604 + wcaD b2056 0.75 +0.8
2,128,612 - wcaD b2056 0.76 -1.1
2,128,612 - wcaD b2056 0.76 -3.1
2,128,612 - wcaD b2056 0.76 -2.9
2,128,614 + wcaD b2056 0.76 -0.9
2,128,617 + wcaD b2056 0.77 +0.4
2,128,667 - wcaD b2056 0.81 -3.3
2,128,668 - wcaD b2056 0.81 -2.9
2,128,690 - wcaD b2056 0.83 -3.5
2,128,696 + wcaD b2056 0.83 -0.3
2,128,696 + wcaD b2056 0.83 -3.2
2,128,696 + wcaD b2056 0.83 -0.2
2,128,702 - wcaD b2056 0.83 -4.6
2,128,704 - wcaD b2056 0.84 -1.2
2,128,713 - wcaD b2056 0.84 -3.2
2,128,722 + wcaD b2056 0.85 -1.4
2,128,918 - -2.0
2,128,918 - -2.7
2,128,925 + -0.8
2,128,957 + -0.5
2,128,968 + -1.0
2,129,087 - wcaC b2057 0.17 -0.8
2,129,249 - wcaC b2057 0.31 -0.7
2,129,249 - wcaC b2057 0.31 +0.4
2,129,459 + wcaC b2057 0.48 -0.6
2,129,459 + wcaC b2057 0.48 -0.9
2,129,468 + wcaC b2057 0.49 -0.9
2,129,473 + wcaC b2057 0.49 -2.4
2,129,586 + wcaC b2057 0.58 -0.7
2,129,690 + wcaC b2057 0.67 -1.9
2,129,798 + wcaC b2057 0.76 -0.5
2,129,803 + wcaC b2057 0.76 -0.5

Or see this region's nucleotide sequence