Strain Fitness in Escherichia coli BW25113 around b0020

Experiment: Laval1374

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaA and nhaR are separated by 59 nucleotidesnhaR and insB-1 are separated by 190 nucleotidesinsB-1 and insA-1 overlap by 82 nucleotides b0019: nhaA - pH-dependent sodium/proton antiporter (NCBI), at 17,489 to 18,655 nhaA b0020: nhaR - DNA-binding transcriptional activator (NCBI), at 18,715 to 19,620 nhaR b0021: insB-1 - IS1 transposase InsAB' (NCBI), at 19,811 to 20,314 insB-1 b0022: insA-1 - IS1 repressor protein InsA (NCBI), at 20,233 to 20,508 insA-1 Position (kb) 18 19 20Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 17.749 kb on - strand, within nhaAat 17.749 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.813 kb on + strand, within nhaAat 17.879 kb on + strand, within nhaAat 17.887 kb on - strand, within nhaAat 17.902 kb on - strand, within nhaAat 17.904 kb on + strand, within nhaAat 17.917 kb on + strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.990 kb on + strand, within nhaAat 17.990 kb on + strand, within nhaAat 18.015 kb on - strand, within nhaAat 18.041 kb on - strand, within nhaAat 18.044 kb on - strand, within nhaAat 18.044 kb on - strand, within nhaAat 18.044 kb on - strand, within nhaAat 18.085 kb on + strand, within nhaAat 18.085 kb on + strand, within nhaAat 18.095 kb on - strand, within nhaAat 18.097 kb on - strandat 18.097 kb on - strand, within nhaAat 18.098 kb on - strand, within nhaAat 18.098 kb on - strand, within nhaAat 18.128 kb on + strand, within nhaAat 18.167 kb on + strand, within nhaAat 18.201 kb on - strand, within nhaAat 18.244 kb on - strand, within nhaAat 18.245 kb on + strand, within nhaAat 18.329 kb on - strand, within nhaAat 18.353 kb on - strand, within nhaAat 18.353 kb on - strand, within nhaAat 18.480 kb on + strand, within nhaAat 18.488 kb on - strand, within nhaAat 18.501 kb on + strand, within nhaAat 18.501 kb on + strand, within nhaAat 18.557 kb on - strandat 18.557 kb on - strandat 18.559 kb on + strandat 18.567 kb on - strandat 18.567 kb on - strandat 18.576 kb on + strandat 18.591 kb on + strandat 18.591 kb on + strandat 18.596 kb on + strandat 18.619 kb on - strandat 18.619 kb on - strandat 18.619 kb on - strandat 18.753 kb on - strandat 18.765 kb on + strandat 18.809 kb on - strand, within nhaRat 18.809 kb on - strand, within nhaRat 18.842 kb on - strand, within nhaRat 18.852 kb on - strand, within nhaRat 18.852 kb on - strand, within nhaRat 18.852 kb on - strand, within nhaRat 18.898 kb on - strand, within nhaRat 18.917 kb on - strand, within nhaRat 18.917 kb on - strand, within nhaRat 19.027 kb on + strand, within nhaRat 19.036 kb on + strand, within nhaRat 19.066 kb on + strand, within nhaRat 19.068 kb on + strand, within nhaRat 19.068 kb on + strand, within nhaRat 19.074 kb on - strand, within nhaRat 19.105 kb on - strand, within nhaRat 19.148 kb on - strand, within nhaRat 19.194 kb on - strand, within nhaRat 19.241 kb on + strand, within nhaRat 19.244 kb on - strand, within nhaRat 19.244 kb on - strand, within nhaRat 19.275 kb on - strand, within nhaRat 19.319 kb on - strand, within nhaRat 19.326 kb on - strand, within nhaRat 19.326 kb on - strand, within nhaRat 19.425 kb on - strand, within nhaRat 19.457 kb on - strand, within nhaRat 19.464 kb on + strand, within nhaRat 19.483 kb on - strand, within nhaRat 19.510 kb on + strand, within nhaRat 19.516 kb on + strand, within nhaRat 19.516 kb on + strand, within nhaRat 19.524 kb on - strand, within nhaRat 19.549 kb on + strandat 19.549 kb on + strandat 19.549 kb on + strandat 19.619 kb on + strandat 19.644 kb on + strandat 19.669 kb on - strandat 19.723 kb on + strandat 19.723 kb on + strandat 19.723 kb on - strandat 20.506 kb on + strandat 20.574 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1374
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17,749 - nhaA b0019 0.22 -3.4
17,749 - nhaA b0019 0.22 -3.0
17,765 - nhaA b0019 0.24 -3.5
17,765 - nhaA b0019 0.24 -2.2
17,813 + nhaA b0019 0.28 -4.3
17,879 + nhaA b0019 0.33 -3.3
17,887 - nhaA b0019 0.34 -3.7
17,902 - nhaA b0019 0.35 -3.6
17,904 + nhaA b0019 0.36 +0.3
17,917 + nhaA b0019 0.37 -3.2
17,925 - nhaA b0019 0.37 -3.9
17,925 - nhaA b0019 0.37 -3.2
17,925 - nhaA b0019 0.37 -3.0
17,990 + nhaA b0019 0.43 -3.8
17,990 + nhaA b0019 0.43 -3.1
18,015 - nhaA b0019 0.45 -4.1
18,041 - nhaA b0019 0.47 -3.8
18,044 - nhaA b0019 0.48 -3.0
18,044 - nhaA b0019 0.48 -2.5
18,044 - nhaA b0019 0.48 -4.0
18,085 + nhaA b0019 0.51 -4.5
18,085 + nhaA b0019 0.51 -1.9
18,095 - nhaA b0019 0.52 -3.3
18,097 - -1.2
18,097 - nhaA b0019 0.52 -1.9
18,098 - nhaA b0019 0.52 -2.8
18,098 - nhaA b0019 0.52 -3.1
18,128 + nhaA b0019 0.55 -3.4
18,167 + nhaA b0019 0.58 -2.1
18,201 - nhaA b0019 0.61 -0.4
18,244 - nhaA b0019 0.65 -2.4
18,245 + nhaA b0019 0.65 -3.3
18,329 - nhaA b0019 0.72 -3.1
18,353 - nhaA b0019 0.74 -2.7
18,353 - nhaA b0019 0.74 -2.4
18,480 + nhaA b0019 0.85 -4.1
18,488 - nhaA b0019 0.86 -4.0
18,501 + nhaA b0019 0.87 -4.5
18,501 + nhaA b0019 0.87 -3.4
18,557 - -1.0
18,557 - -1.6
18,559 + -4.2
18,567 - -1.0
18,567 - -3.1
18,576 + -1.4
18,591 + -4.8
18,591 + -4.8
18,596 + -3.3
18,619 - -1.9
18,619 - -3.8
18,619 - -2.8
18,753 - -0.5
18,765 + -1.7
18,809 - nhaR b0020 0.10 -1.0
18,809 - nhaR b0020 0.10 +0.2
18,842 - nhaR b0020 0.14 -0.5
18,852 - nhaR b0020 0.15 -0.3
18,852 - nhaR b0020 0.15 -1.3
18,852 - nhaR b0020 0.15 -0.8
18,898 - nhaR b0020 0.20 -0.5
18,917 - nhaR b0020 0.22 -0.7
18,917 - nhaR b0020 0.22 -1.2
19,027 + nhaR b0020 0.34 -2.0
19,036 + nhaR b0020 0.35 -1.2
19,066 + nhaR b0020 0.39 -0.1
19,068 + nhaR b0020 0.39 -0.2
19,068 + nhaR b0020 0.39 -0.8
19,074 - nhaR b0020 0.40 -1.5
19,105 - nhaR b0020 0.43 -3.1
19,148 - nhaR b0020 0.48 +0.1
19,194 - nhaR b0020 0.53 -0.8
19,241 + nhaR b0020 0.58 -1.3
19,244 - nhaR b0020 0.58 -0.8
19,244 - nhaR b0020 0.58 -0.5
19,275 - nhaR b0020 0.62 -0.8
19,319 - nhaR b0020 0.67 -0.6
19,326 - nhaR b0020 0.67 -0.8
19,326 - nhaR b0020 0.67 -0.8
19,425 - nhaR b0020 0.78 -0.5
19,457 - nhaR b0020 0.82 -0.4
19,464 + nhaR b0020 0.83 -1.2
19,483 - nhaR b0020 0.85 -0.4
19,510 + nhaR b0020 0.88 +0.6
19,516 + nhaR b0020 0.88 -0.5
19,516 + nhaR b0020 0.88 -0.1
19,524 - nhaR b0020 0.89 -1.2
19,549 + -2.0
19,549 + -1.7
19,549 + -1.4
19,619 + -1.0
19,644 + +1.1
19,669 - -0.1
19,723 + +1.1
19,723 + -0.5
19,723 - -1.6
20,506 + -0.4
20,574 - -0.3

Or see this region's nucleotide sequence