Strain Fitness in Escherichia coli BW25113 around b0157

Experiment: TN_15CS_15C_10dbl_rep3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclcA and yadR are separated by 81 nucleotidesyadR and yadS are separated by 46 nucleotidesyadS and btuF are separated by 37 nucleotidesbtuF and pfs overlap by 8 nucleotides b0155: clcA - chloride channel protein (NCBI), at 175,107 to 176,528 clcA b0156: yadR - hypothetical protein (NCBI), at 176,610 to 176,954 yadR b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624 yadS b0158: btuF - vitamin B12-transporter protein BtuF (NCBI), at 177,662 to 178,462 btuF b0159: pfs - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI), at 178,455 to 179,153 pfs Position (kb) 177 178Strain fitness (log2 ratio) -2 -1 0 1at 176.017 kb on - strand, within clcAat 176.017 kb on - strand, within clcAat 176.136 kb on + strand, within clcAat 176.173 kb on + strand, within clcAat 176.210 kb on - strand, within clcAat 176.210 kb on - strand, within clcAat 176.295 kb on + strand, within clcAat 176.356 kb on + strand, within clcAat 176.441 kb on - strandat 176.503 kb on - strandat 176.503 kb on - strandat 176.514 kb on + strandat 176.540 kb on + strandat 177.002 kb on - strandat 177.019 kb on - strandat 177.019 kb on - strandat 177.084 kb on - strand, within yadSat 177.084 kb on - strand, within yadSat 177.084 kb on - strand, within yadSat 177.084 kb on - strand, within yadSat 177.084 kb on - strand, within yadSat 177.084 kb on - strand, within yadSat 177.221 kb on - strand, within yadSat 177.257 kb on + strand, within yadSat 177.300 kb on - strand, within yadSat 177.300 kb on - strand, within yadSat 177.333 kb on + strand, within yadSat 177.341 kb on - strand, within yadSat 177.531 kb on + strand, within yadSat 177.578 kb on + strandat 177.596 kb on - strandat 177.596 kb on - strandat 177.616 kb on + strandat 177.616 kb on + strandat 177.625 kb on + strandat 177.625 kb on + strandat 177.697 kb on + strandat 177.714 kb on - strandat 177.726 kb on + strandat 177.735 kb on - strandat 178.094 kb on - strand, within btuFat 178.094 kb on - strand, within btuFat 178.138 kb on + strand, within btuFat 178.138 kb on - strand, within btuFat 178.150 kb on - strand, within btuFat 178.150 kb on - strand, within btuFat 178.304 kb on - strand, within btuFat 178.304 kb on - strand, within btuFat 178.333 kb on + strand, within btuFat 178.440 kb on + strandat 178.440 kb on + strandat 178.448 kb on - strandat 178.452 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TN_15CS_15C_10dbl_rep3
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176,017 - clcA b0155 0.64 -0.4
176,017 - clcA b0155 0.64 -0.7
176,136 + clcA b0155 0.72 -0.9
176,173 + clcA b0155 0.75 -0.6
176,210 - clcA b0155 0.78 +0.1
176,210 - clcA b0155 0.78 -0.6
176,295 + clcA b0155 0.84 +0.3
176,356 + clcA b0155 0.88 +0.5
176,441 - -1.2
176,503 - +0.1
176,503 - -1.8
176,514 + -0.1
176,540 + +0.3
177,002 - -0.1
177,019 - -1.2
177,019 - -0.6
177,084 - yadS b0157 0.13 -0.8
177,084 - yadS b0157 0.13 -0.6
177,084 - yadS b0157 0.13 -0.4
177,084 - yadS b0157 0.13 -1.0
177,084 - yadS b0157 0.13 -0.6
177,084 - yadS b0157 0.13 -0.9
177,221 - yadS b0157 0.35 -0.3
177,257 + yadS b0157 0.41 -0.1
177,300 - yadS b0157 0.48 -1.8
177,300 - yadS b0157 0.48 -1.0
177,333 + yadS b0157 0.53 -0.4
177,341 - yadS b0157 0.54 -0.2
177,531 + yadS b0157 0.85 -0.8
177,578 + -1.3
177,596 - -0.7
177,596 - -0.3
177,616 + -1.7
177,616 + -0.6
177,625 + +0.5
177,625 + +0.2
177,697 + -0.5
177,714 - +0.9
177,726 + -1.1
177,735 - +0.4
178,094 - btuF b0158 0.54 +0.2
178,094 - btuF b0158 0.54 +0.1
178,138 + btuF b0158 0.59 -0.9
178,138 - btuF b0158 0.59 +0.7
178,150 - btuF b0158 0.61 +0.4
178,150 - btuF b0158 0.61 +1.1
178,304 - btuF b0158 0.80 +0.3
178,304 - btuF b0158 0.80 +0.2
178,333 + btuF b0158 0.84 -0.2
178,440 + -0.3
178,440 + -0.4
178,448 - +0.1
178,452 + -0.1

Or see this region's nucleotide sequence