Strain Fitness in Escherichia coli BW25113 around b1627

Experiment: TN_15CS_15C_5dbl_rep2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgT and b1626 are separated by 85 nucleotidesb1626 and rsxA are separated by 76 nucleotidesrsxA and rsxB overlap by 1 nucleotidesrsxB and rsxC overlap by 8 nucleotides b1625: ydgT - predicted regulator (NCBI), at 1,702,973 to 1,703,188 ydgT b1626: b1626 - orf, hypothetical protein (VIMSS), at 1,703,274 to 1,703,714 b1626 b1627: rsxA - Na(+)-translocating NADH-quinone reductase subunit E (NCBI), at 1,703,791 to 1,704,372 rsxA b1628: rsxB - electron transport complex protein RnfB (NCBI), at 1,704,372 to 1,704,950 rsxB b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165 rsxC Position (kb) 1703 1704 1705Strain fitness (log2 ratio) -2 -1 0 1 2at 1702.812 kb on + strandat 1702.812 kb on + strandat 1702.821 kb on + strandat 1702.979 kb on + strandat 1702.984 kb on + strandat 1702.984 kb on + strandat 1702.987 kb on - strandat 1702.987 kb on - strandat 1702.987 kb on - strandat 1702.990 kb on - strandat 1702.992 kb on - strandat 1703.033 kb on - strand, within ydgTat 1703.052 kb on + strand, within ydgTat 1703.054 kb on - strand, within ydgTat 1703.054 kb on - strand, within ydgTat 1703.055 kb on + strand, within ydgTat 1703.070 kb on + strand, within ydgTat 1703.070 kb on + strand, within ydgTat 1703.070 kb on + strand, within ydgTat 1703.070 kb on + strand, within ydgTat 1703.070 kb on + strand, within ydgTat 1703.078 kb on - strand, within ydgTat 1703.078 kb on - strand, within ydgTat 1703.081 kb on + strand, within ydgTat 1703.084 kb on + strand, within ydgTat 1703.089 kb on + strand, within ydgTat 1703.092 kb on - strand, within ydgTat 1703.102 kb on - strand, within ydgTat 1703.105 kb on - strand, within ydgTat 1703.109 kb on + strand, within ydgTat 1703.109 kb on + strand, within ydgTat 1703.142 kb on - strand, within ydgTat 1703.153 kb on - strand, within ydgTat 1703.178 kb on + strandat 1703.210 kb on - strandat 1703.263 kb on - strandat 1703.268 kb on - strandat 1703.276 kb on + strandat 1703.278 kb on - strandat 1703.296 kb on + strandat 1703.296 kb on + strandat 1703.368 kb on - strand, within b1626at 1703.368 kb on - strand, within b1626at 1703.369 kb on + strand, within b1626at 1703.377 kb on - strand, within b1626at 1703.377 kb on - strand, within b1626at 1703.377 kb on - strand, within b1626at 1703.380 kb on - strand, within b1626at 1703.380 kb on - strand, within b1626at 1703.424 kb on + strand, within b1626at 1703.427 kb on - strand, within b1626at 1703.427 kb on - strand, within b1626at 1703.480 kb on + strand, within b1626at 1703.495 kb on + strand, within b1626at 1703.503 kb on - strand, within b1626at 1703.518 kb on - strand, within b1626at 1703.518 kb on - strand, within b1626at 1703.527 kb on + strand, within b1626at 1703.537 kb on + strand, within b1626at 1703.542 kb on + strand, within b1626at 1703.542 kb on + strand, within b1626at 1703.549 kb on - strand, within b1626at 1703.609 kb on + strand, within b1626at 1703.617 kb on - strand, within b1626at 1703.670 kb on + strand, within b1626at 1703.671 kb on - strandat 1703.671 kb on - strandat 1703.819 kb on + strandat 1703.827 kb on - strandat 1703.845 kb on - strandat 1703.847 kb on + strandat 1703.861 kb on + strand, within rsxAat 1703.861 kb on + strand, within rsxAat 1703.908 kb on - strand, within rsxAat 1704.048 kb on + strand, within rsxAat 1704.090 kb on - strand, within rsxAat 1704.235 kb on - strand, within rsxAat 1704.376 kb on + strandat 1704.376 kb on + strandat 1704.377 kb on - strandat 1704.525 kb on - strand, within rsxBat 1704.772 kb on + strand, within rsxBat 1704.806 kb on + strand, within rsxBat 1705.039 kb on + strandat 1705.042 kb on - strandat 1705.235 kb on - strand, within rsxCat 1705.290 kb on + strand, within rsxCat 1705.290 kb on + strand, within rsxCat 1705.308 kb on - strand, within rsxCat 1705.308 kb on - strand, within rsxC

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Per-strain Table

Position Strand Gene LocusTag Fraction TN_15CS_15C_5dbl_rep2
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1,702,812 + +0.2
1,702,812 + -0.5
1,702,821 + -0.6
1,702,979 + +1.0
1,702,984 + +0.7
1,702,984 + +1.8
1,702,987 - +1.1
1,702,987 - -0.8
1,702,987 - -0.2
1,702,990 - +0.1
1,702,992 - +1.2
1,703,033 - ydgT b1625 0.28 +1.1
1,703,052 + ydgT b1625 0.37 +0.4
1,703,054 - ydgT b1625 0.38 -0.0
1,703,054 - ydgT b1625 0.38 -0.5
1,703,055 + ydgT b1625 0.38 +0.3
1,703,070 + ydgT b1625 0.45 -0.1
1,703,070 + ydgT b1625 0.45 +1.0
1,703,070 + ydgT b1625 0.45 -0.4
1,703,070 + ydgT b1625 0.45 +0.1
1,703,070 + ydgT b1625 0.45 +0.8
1,703,078 - ydgT b1625 0.49 +0.7
1,703,078 - ydgT b1625 0.49 -2.1
1,703,081 + ydgT b1625 0.50 -0.9
1,703,084 + ydgT b1625 0.51 +0.2
1,703,089 + ydgT b1625 0.54 -0.2
1,703,092 - ydgT b1625 0.55 -0.6
1,703,102 - ydgT b1625 0.60 -0.3
1,703,105 - ydgT b1625 0.61 -0.7
1,703,109 + ydgT b1625 0.63 +0.4
1,703,109 + ydgT b1625 0.63 +0.8
1,703,142 - ydgT b1625 0.78 -0.4
1,703,153 - ydgT b1625 0.83 -0.4
1,703,178 + +0.7
1,703,210 - -0.0
1,703,263 - +0.2
1,703,268 - -1.0
1,703,276 + -0.3
1,703,278 - -0.1
1,703,296 + -1.6
1,703,296 + -0.8
1,703,368 - b1626 b1626 0.21 -0.4
1,703,368 - b1626 b1626 0.21 +0.3
1,703,369 + b1626 b1626 0.22 -0.3
1,703,377 - b1626 b1626 0.23 -2.3
1,703,377 - b1626 b1626 0.23 +0.4
1,703,377 - b1626 b1626 0.23 -1.1
1,703,380 - b1626 b1626 0.24 -0.7
1,703,380 - b1626 b1626 0.24 +0.2
1,703,424 + b1626 b1626 0.34 -0.0
1,703,427 - b1626 b1626 0.35 -1.3
1,703,427 - b1626 b1626 0.35 +0.6
1,703,480 + b1626 b1626 0.47 -1.0
1,703,495 + b1626 b1626 0.50 -0.3
1,703,503 - b1626 b1626 0.52 +0.6
1,703,518 - b1626 b1626 0.55 +0.3
1,703,518 - b1626 b1626 0.55 +0.2
1,703,527 + b1626 b1626 0.57 +0.1
1,703,537 + b1626 b1626 0.60 -1.4
1,703,542 + b1626 b1626 0.61 +0.8
1,703,542 + b1626 b1626 0.61 +0.1
1,703,549 - b1626 b1626 0.62 -0.1
1,703,609 + b1626 b1626 0.76 -0.2
1,703,617 - b1626 b1626 0.78 -0.9
1,703,670 + b1626 b1626 0.90 -0.5
1,703,671 - -0.1
1,703,671 - +0.2
1,703,819 + -0.7
1,703,827 - -0.4
1,703,845 - +1.0
1,703,847 + +0.9
1,703,861 + rsxA b1627 0.12 +0.2
1,703,861 + rsxA b1627 0.12 +0.5
1,703,908 - rsxA b1627 0.20 -0.2
1,704,048 + rsxA b1627 0.44 -0.1
1,704,090 - rsxA b1627 0.51 -0.1
1,704,235 - rsxA b1627 0.76 -0.4
1,704,376 + +0.7
1,704,376 + -0.1
1,704,377 - -0.4
1,704,525 - rsxB b1628 0.26 -0.3
1,704,772 + rsxB b1628 0.69 -0.3
1,704,806 + rsxB b1628 0.75 -0.2
1,705,039 + -0.0
1,705,042 - -1.5
1,705,235 - rsxC b1629 0.13 -0.3
1,705,290 + rsxC b1629 0.16 -0.4
1,705,290 + rsxC b1629 0.16 +1.3
1,705,308 - rsxC b1629 0.16 -0.0
1,705,308 - rsxC b1629 0.16 +0.8

Or see this region's nucleotide sequence