Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sprT and endA are separated by 94 nucleotides endA and yggJ are separated by 79 nucleotides yggJ and gshB are separated by 12 nucleotides
b2944: sprT - hypothetical protein (NCBI), at 3,087,777 to 3,088,274
sprT
b2945: endA - DNA-specific endonuclease I (NCBI), at 3,088,369 to 3,089,076
endA
b2946: yggJ - orf, hypothetical protein (VIMSS), at 3,089,156 to 3,089,887
yggJ
b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850
gshB
Position (kb)
3089
3090 Strain fitness (log2 ratio)
-2
-1
0
1 at 3088.350 kb on + strand at 3088.350 kb on + strand at 3088.406 kb on - strand at 3088.406 kb on - strand at 3088.453 kb on + strand, within endA at 3088.619 kb on - strand, within endA at 3088.683 kb on - strand, within endA at 3088.724 kb on + strand, within endA at 3088.732 kb on - strand, within endA at 3088.732 kb on - strand, within endA at 3088.875 kb on - strand, within endA at 3088.885 kb on - strand, within endA at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.970 kb on - strand, within endA at 3088.970 kb on - strand, within endA at 3089.020 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.191 kb on + strand at 3089.281 kb on - strand, within yggJ at 3089.295 kb on - strand, within yggJ at 3089.411 kb on + strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.895 kb on + strand at 3089.924 kb on - strand at 3090.004 kb on + strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.092 kb on - strand, within gshB at 3090.222 kb on + strand, within gshB at 3090.458 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.486 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.576 kb on + strand, within gshB at 3090.811 kb on + strand at 3090.847 kb on - strand at 3090.847 kb on - strand at 3090.883 kb on + strand at 3090.883 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 3,088,350 + -0.1 3,088,350 + -0.9 3,088,406 - -0.5 3,088,406 - +0.2 3,088,453 + endA b2945 0.12 -0.3 3,088,619 - endA b2945 0.35 +0.4 3,088,683 - endA b2945 0.44 +0.2 3,088,724 + endA b2945 0.50 -0.2 3,088,732 - endA b2945 0.51 -0.1 3,088,732 - endA b2945 0.51 +0.1 3,088,875 - endA b2945 0.71 +0.1 3,088,885 - endA b2945 0.73 -0.0 3,088,962 + endA b2945 0.84 -0.2 3,088,962 + endA b2945 0.84 -0.0 3,088,962 + endA b2945 0.84 -0.9 3,088,970 - endA b2945 0.85 -0.4 3,088,970 - endA b2945 0.85 +0.1 3,089,020 - +0.2 3,089,038 - +0.2 3,089,038 - +0.1 3,089,038 - +0.0 3,089,191 + +0.6 3,089,281 - yggJ b2946 0.17 +0.9 3,089,295 - yggJ b2946 0.19 +0.9 3,089,411 + yggJ b2946 0.35 +0.4 3,089,476 - yggJ b2946 0.44 +1.1 3,089,476 - yggJ b2946 0.44 +0.6 3,089,586 - yggJ b2946 0.59 +0.0 3,089,586 - yggJ b2946 0.59 +0.7 3,089,634 + yggJ b2946 0.65 +0.7 3,089,634 + yggJ b2946 0.65 +0.2 3,089,642 - yggJ b2946 0.66 +1.3 3,089,642 - yggJ b2946 0.66 +0.9 3,089,895 + +0.1 3,089,924 - -0.9 3,090,004 + gshB b2947 0.11 -1.9 3,090,085 - gshB b2947 0.19 -1.3 3,090,085 - gshB b2947 0.19 -0.4 3,090,092 - gshB b2947 0.20 -0.9 3,090,222 + gshB b2947 0.34 -0.3 3,090,458 - gshB b2947 0.59 -1.0 3,090,479 - gshB b2947 0.61 -1.1 3,090,479 - gshB b2947 0.61 -1.1 3,090,486 - gshB b2947 0.62 -1.9 3,090,562 - gshB b2947 0.70 -1.1 3,090,562 - gshB b2947 0.70 -1.0 3,090,576 + gshB b2947 0.71 -1.5 3,090,811 + -0.4 3,090,847 - -1.0 3,090,847 - +0.3 3,090,883 + -0.3 3,090,883 + -1.0
Or see this region's nucleotide sequence