Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pbpG and yohC are separated by 173 nucleotides yohC and yohD are separated by 169 nucleotides yohD and yohF are separated by 129 nucleotides yohF and mdtQ are separated by 52 nucleotides
b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892
pbpG
b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653
yohC
b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401
yohD
b2137: yohF - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,224,531 to 2,225,292
yohF
b2139: mdtQ - orf, hypothetical protein (VIMSS), at 2,225,345 to 2,226,780
mdtQ
Position (kb)
2223
2224
2225 Strain fitness (log2 ratio)
-1
0
1
2 at 2222.976 kb on - strand at 2223.193 kb on - strand, within yohC at 2223.323 kb on - strand, within yohC at 2223.457 kb on - strand, within yohC at 2223.476 kb on - strand, within yohC at 2223.531 kb on + strand, within yohC at 2223.573 kb on - strand, within yohC at 2223.647 kb on + strand at 2223.831 kb on + strand at 2223.879 kb on + strand at 2224.007 kb on - strand, within yohD at 2224.171 kb on - strand, within yohD at 2224.249 kb on - strand, within yohD at 2224.348 kb on - strand at 2224.358 kb on + strand at 2224.417 kb on - strand at 2224.529 kb on - strand at 2224.541 kb on - strand at 2224.578 kb on + strand at 2224.713 kb on - strand, within yohF at 2224.872 kb on - strand, within yohF at 2224.902 kb on + strand, within yohF at 2224.902 kb on + strand, within yohF at 2224.942 kb on + strand, within yohF at 2224.992 kb on - strand, within yohF at 2224.992 kb on - strand, within yohF at 2224.994 kb on - strand, within yohF at 2224.994 kb on - strand, within yohF at 2225.063 kb on - strand, within yohF at 2225.103 kb on - strand, within yohF at 2225.133 kb on + strand, within yohF at 2225.163 kb on + strand, within yohF at 2225.199 kb on - strand, within yohF at 2225.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 2,222,976 - +0.3 2,223,193 - yohC b2135 0.22 +0.1 2,223,323 - yohC b2135 0.44 +0.1 2,223,457 - yohC b2135 0.66 -0.2 2,223,476 - yohC b2135 0.70 -0.1 2,223,531 + yohC b2135 0.79 -0.2 2,223,573 - yohC b2135 0.86 -0.4 2,223,647 + -0.3 2,223,831 + -0.3 2,223,879 + +0.0 2,224,007 - yohD b2136 0.32 +1.4 2,224,171 - yohD b2136 0.60 +0.5 2,224,249 - yohD b2136 0.74 -0.1 2,224,348 - +0.7 2,224,358 + +1.3 2,224,417 - +0.4 2,224,529 - -0.5 2,224,541 - -0.1 2,224,578 + -0.3 2,224,713 - yohF b2137 0.24 -0.6 2,224,872 - yohF b2137 0.45 -1.3 2,224,902 + yohF b2137 0.49 +2.2 2,224,902 + yohF b2137 0.49 -0.7 2,224,942 + yohF b2137 0.54 -0.2 2,224,992 - yohF b2137 0.60 -0.2 2,224,992 - yohF b2137 0.60 +0.0 2,224,994 - yohF b2137 0.61 -0.1 2,224,994 - yohF b2137 0.61 -0.3 2,225,063 - yohF b2137 0.70 -0.1 2,225,103 - yohF b2137 0.75 -0.1 2,225,133 + yohF b2137 0.79 -0.4 2,225,163 + yohF b2137 0.83 +0.6 2,225,199 - yohF b2137 0.88 -0.9 2,225,292 - +0.0
Or see this region's nucleotide sequence