Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliF and fliG overlap by 8 nucleotides fliG and fliH overlap by 8 nucleotides fliH and fliI overlap by 1 nucleotides
b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911
fliF
b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899
fliG
b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578
fliH
b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951
fliI
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-1
0
1 at 2012.922 kb on + strand at 2012.979 kb on + strand at 2012.979 kb on + strand at 2013.108 kb on + strand, within fliG at 2013.108 kb on + strand, within fliG at 2013.149 kb on - strand, within fliG at 2013.444 kb on + strand, within fliG at 2014.036 kb on + strand, within fliH at 2014.099 kb on - strand, within fliH at 2014.120 kb on + strand, within fliH at 2014.214 kb on + strand, within fliH at 2014.240 kb on - strand, within fliH at 2014.246 kb on + strand, within fliH at 2014.318 kb on - strand, within fliH at 2014.336 kb on + strand, within fliH at 2014.404 kb on - strand, within fliH at 2014.562 kb on + strand at 2014.562 kb on + strand at 2014.829 kb on + strand, within fliI at 2014.829 kb on + strand, within fliI at 2014.917 kb on - strand, within fliI at 2014.920 kb on + strand, within fliI at 2014.920 kb on + strand, within fliI at 2015.057 kb on - strand, within fliI at 2015.057 kb on - strand at 2015.259 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.499 kb on - strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.560 kb on + strand, within fliI
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 2,012,922 + -0.7 2,012,979 + -0.6 2,012,979 + -0.2 2,013,108 + fliG b1939 0.20 +0.1 2,013,108 + fliG b1939 0.20 -0.0 2,013,149 - fliG b1939 0.25 -0.7 2,013,444 + fliG b1939 0.54 +0.4 2,014,036 + fliH b1940 0.21 -0.1 2,014,099 - fliH b1940 0.30 +0.4 2,014,120 + fliH b1940 0.33 +0.3 2,014,214 + fliH b1940 0.47 +0.2 2,014,240 - fliH b1940 0.51 +0.4 2,014,246 + fliH b1940 0.52 +0.5 2,014,318 - fliH b1940 0.62 +0.2 2,014,336 + fliH b1940 0.65 +0.4 2,014,404 - fliH b1940 0.75 -0.2 2,014,562 + -1.7 2,014,562 + -0.1 2,014,829 + fliI b1941 0.18 -0.7 2,014,829 + fliI b1941 0.18 -0.6 2,014,917 - fliI b1941 0.25 +0.1 2,014,920 + fliI b1941 0.25 -0.4 2,014,920 + fliI b1941 0.25 +0.5 2,015,057 - fliI b1941 0.35 +0.3 2,015,057 - -0.3 2,015,259 - fliI b1941 0.50 +0.2 2,015,261 - fliI b1941 0.50 +0.7 2,015,261 - fliI b1941 0.50 -0.1 2,015,499 - fliI b1941 0.67 -0.4 2,015,549 + fliI b1941 0.71 -0.5 2,015,549 + fliI b1941 0.71 -0.1 2,015,560 + fliI b1941 0.71 -0.1
Or see this region's nucleotide sequence