Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1757 and b1758 are separated by 8 nucleotides b1758 and nudG are separated by 86 nucleotides nudG and ynjH overlap by 35 nucleotides ynjH and gdhA are separated by 235 nucleotides
b1757: b1757 - putative thiosulfate sulfur transferase (VIMSS), at 1,837,491 to 1,838,798
b1757
b1758: b1758 - putative cytochrome oxidase (VIMSS), at 1,838,807 to 1,839,427
b1758
b1759: nudG - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase (NCBI), at 1,839,514 to 1,839,921
nudG
b1760: ynjH - hypothetical protein (NCBI), at 1,839,887 to 1,840,159
ynjH
b1761: gdhA - glutamate dehydrogenase (NCBI), at 1,840,395 to 1,841,738
gdhA
Position (kb)
1838
1839
1840 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1838.008 kb on - strand, within b1757 at 1838.022 kb on + strand, within b1757 at 1838.065 kb on - strand, within b1757 at 1838.179 kb on + strand, within b1757 at 1838.194 kb on - strand, within b1757 at 1838.463 kb on + strand, within b1757 at 1838.563 kb on + strand, within b1757 at 1838.833 kb on + strand at 1838.833 kb on + strand at 1839.201 kb on + strand, within b1758 at 1839.552 kb on - strand at 1839.552 kb on - strand at 1839.760 kb on - strand, within nudG at 1839.893 kb on - strand at 1840.011 kb on + strand, within ynjH at 1840.026 kb on - strand, within ynjH at 1840.029 kb on - strand, within ynjH at 1840.031 kb on - strand, within ynjH at 1840.258 kb on - strand at 1840.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 1,838,008 - b1757 b1757 0.40 -0.7 1,838,022 + b1757 b1757 0.41 -0.1 1,838,065 - b1757 b1757 0.44 -0.7 1,838,179 + b1757 b1757 0.53 +0.2 1,838,194 - b1757 b1757 0.54 +0.3 1,838,463 + b1757 b1757 0.74 +0.7 1,838,563 + b1757 b1757 0.82 +0.1 1,838,833 + +1.5 1,838,833 + +3.2 1,839,201 + b1758 b1758 0.63 +0.4 1,839,552 - -0.2 1,839,552 - +0.3 1,839,760 - nudG b1759 0.60 -0.6 1,839,893 - -0.1 1,840,011 + ynjH b1760 0.45 +1.0 1,840,026 - ynjH b1760 0.51 -0.0 1,840,029 - ynjH b1760 0.52 -0.1 1,840,031 - ynjH b1760 0.53 +0.4 1,840,258 - -0.2 1,840,325 - +0.4
Or see this region's nucleotide sequence