Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-4 and ynaJ are separated by 272 nucleotides ynaJ and uspE are separated by 49 nucleotides uspE and fnr are separated by 151 nucleotides
b1331: insH-4 - IS5 transposase and trans-activator (NCBI), at 1,394,100 to 1,395,116
insH-4
b1332: ynaJ - predicted inner membrane protein (NCBI), at 1,395,389 to 1,395,646
ynaJ
b1333: uspE - stress-induced protein (NCBI), at 1,395,696 to 1,396,646
uspE
b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550
fnr
Position (kb)
1395
1396
1397 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1395.516 kb on - strand, within ynaJ at 1395.618 kb on - strand, within ynaJ at 1395.682 kb on + strand at 1395.748 kb on - strand at 1396.148 kb on + strand, within uspE at 1396.214 kb on - strand, within uspE at 1396.295 kb on - strand, within uspE at 1396.341 kb on + strand, within uspE at 1396.604 kb on + strand at 1396.609 kb on + strand at 1396.609 kb on + strand at 1396.728 kb on - strand at 1396.728 kb on - strand at 1397.086 kb on + strand, within fnr at 1397.086 kb on + strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.287 kb on - strand, within fnr at 1397.605 kb on + strand at 1397.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 1,395,516 - ynaJ b1332 0.49 +3.6 1,395,618 - ynaJ b1332 0.89 -1.1 1,395,682 + +0.8 1,395,748 - -0.2 1,396,148 + uspE b1333 0.48 +1.0 1,396,214 - uspE b1333 0.54 -0.3 1,396,295 - uspE b1333 0.63 +0.7 1,396,341 + uspE b1333 0.68 +0.3 1,396,604 + +0.5 1,396,609 + +0.7 1,396,609 + -1.1 1,396,728 - +0.6 1,396,728 - -0.4 1,397,086 + fnr b1334 0.38 -0.1 1,397,086 + fnr b1334 0.38 +0.5 1,397,125 - fnr b1334 0.43 -2.1 1,397,125 - fnr b1334 0.43 +0.9 1,397,287 - fnr b1334 0.65 -0.1 1,397,605 + -0.5 1,397,605 + -0.3
Or see this region's nucleotide sequence