Experiment: TN_18CS_warm37C_5dbl_rep3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wrbA and ycdF are separated by 372 nucleotides ycdF and ycdG are separated by 256 nucleotides ycdG and b1007 are separated by 20 nucleotides b1007 and ycdI are separated by 10 nucleotides
b1004: wrbA - TrpR binding protein WrbA (NCBI), at 1,066,335 to 1,066,931
wrbA
b4518: ycdF - orf, hypothetical protein (VIMSS), at 1,067,304 to 1,067,477
ycdF
b1006: ycdG - putative transport protein (VIMSS), at 1,067,734 to 1,069,062
ycdG
b1007: b1007 - orf, hypothetical protein (VIMSS), at 1,069,083 to 1,069,577
b1007
b1008: ycdI - hypothetical protein (NCBI), at 1,069,588 to 1,070,178
ycdI
Position (kb)
1067
1068
1069
1070 Strain fitness (log2 ratio)
-1
0
1
2 at 1066.821 kb on - strand, within wrbA at 1066.849 kb on - strand, within wrbA at 1066.911 kb on - strand at 1066.947 kb on + strand at 1066.951 kb on - strand at 1067.176 kb on - strand at 1067.203 kb on - strand at 1067.203 kb on - strand at 1067.205 kb on + strand at 1067.212 kb on - strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.279 kb on + strand at 1067.302 kb on - strand at 1067.589 kb on + strand at 1067.833 kb on - strand at 1067.883 kb on + strand, within ycdG at 1067.922 kb on + strand, within ycdG at 1068.096 kb on - strand, within ycdG at 1068.544 kb on - strand, within ycdG at 1068.547 kb on + strand, within ycdG at 1068.622 kb on + strand, within ycdG at 1068.622 kb on + strand, within ycdG at 1068.702 kb on + strand, within ycdG at 1068.702 kb on + strand, within ycdG at 1068.921 kb on - strand, within ycdG at 1068.921 kb on - strand, within ycdG at 1068.970 kb on - strand at 1068.999 kb on + strand at 1069.014 kb on + strand at 1069.046 kb on + strand at 1069.046 kb on + strand at 1069.126 kb on - strand at 1069.242 kb on + strand, within b1007 at 1069.600 kb on + strand at 1069.660 kb on + strand, within ycdI at 1069.662 kb on - strand, within ycdI at 1069.662 kb on - strand, within ycdI at 1069.797 kb on + strand, within ycdI at 1069.797 kb on + strand, within ycdI at 1069.870 kb on - strand, within ycdI at 1069.870 kb on - strand, within ycdI at 1069.872 kb on + strand, within ycdI at 1069.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_warm37C_5dbl_rep3 remove 1,066,821 - wrbA b1004 0.81 +0.2 1,066,849 - wrbA b1004 0.86 +0.6 1,066,911 - -0.0 1,066,947 + +0.3 1,066,951 - +0.2 1,067,176 - +1.2 1,067,203 - -0.1 1,067,203 - -0.5 1,067,205 + +0.3 1,067,212 - -0.3 1,067,214 + -1.4 1,067,214 + +0.3 1,067,214 + +0.1 1,067,214 + -0.2 1,067,254 - +1.1 1,067,254 - +0.2 1,067,254 - -0.3 1,067,254 - +0.9 1,067,254 - -0.0 1,067,279 + -0.4 1,067,302 - -0.3 1,067,589 + +0.6 1,067,833 - +0.4 1,067,883 + ycdG b1006 0.11 -0.1 1,067,922 + ycdG b1006 0.14 +0.2 1,068,096 - ycdG b1006 0.27 -0.4 1,068,544 - ycdG b1006 0.61 +0.6 1,068,547 + ycdG b1006 0.61 +0.9 1,068,622 + ycdG b1006 0.67 +2.3 1,068,622 + ycdG b1006 0.67 -0.0 1,068,702 + ycdG b1006 0.73 +0.9 1,068,702 + ycdG b1006 0.73 -1.2 1,068,921 - ycdG b1006 0.89 +0.1 1,068,921 - ycdG b1006 0.89 +0.1 1,068,970 - +0.8 1,068,999 + -0.1 1,069,014 + +0.7 1,069,046 + +0.1 1,069,046 + -1.3 1,069,126 - +0.0 1,069,242 + b1007 b1007 0.32 -0.3 1,069,600 + -0.1 1,069,660 + ycdI b1008 0.12 -0.7 1,069,662 - ycdI b1008 0.13 +0.1 1,069,662 - ycdI b1008 0.13 -0.9 1,069,797 + ycdI b1008 0.35 -0.3 1,069,797 + ycdI b1008 0.35 -0.0 1,069,870 - ycdI b1008 0.48 -0.7 1,069,870 - ycdI b1008 0.48 +1.0 1,069,872 + ycdI b1008 0.48 +0.5 1,069,872 + +0.0
Or see this region's nucleotide sequence