Experiment: TN_18CS_18C_5dbl_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clcA and yadR are separated by 81 nucleotides yadR and yadS are separated by 46 nucleotides yadS and btuF are separated by 37 nucleotides btuF and pfs overlap by 8 nucleotides
b0155: clcA - chloride channel protein (NCBI), at 175,107 to 176,528
clcA
b0156: yadR - hypothetical protein (NCBI), at 176,610 to 176,954
yadR
b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624
yadS
b0158: btuF - vitamin B12-transporter protein BtuF (NCBI), at 177,662 to 178,462
btuF
b0159: pfs - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI), at 178,455 to 179,153
pfs
Position (kb)
177
178 Strain fitness (log2 ratio)
-1
0
1 at 176.017 kb on - strand, within clcA at 176.017 kb on - strand, within clcA at 176.136 kb on + strand, within clcA at 176.173 kb on + strand, within clcA at 176.210 kb on - strand, within clcA at 176.210 kb on - strand, within clcA at 176.295 kb on + strand, within clcA at 176.356 kb on + strand, within clcA at 176.441 kb on - strand at 176.503 kb on - strand at 176.503 kb on - strand at 176.514 kb on + strand at 176.540 kb on + strand at 177.002 kb on - strand at 177.019 kb on - strand at 177.019 kb on - strand at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.221 kb on - strand, within yadS at 177.257 kb on + strand, within yadS at 177.300 kb on - strand, within yadS at 177.300 kb on - strand, within yadS at 177.333 kb on + strand, within yadS at 177.341 kb on - strand, within yadS at 177.531 kb on + strand, within yadS at 177.578 kb on + strand at 177.596 kb on - strand at 177.596 kb on - strand at 177.616 kb on + strand at 177.616 kb on + strand at 177.625 kb on + strand at 177.625 kb on + strand at 177.697 kb on + strand at 177.714 kb on - strand at 177.726 kb on + strand at 177.735 kb on - strand at 178.094 kb on - strand, within btuF at 178.094 kb on - strand, within btuF at 178.138 kb on + strand, within btuF at 178.138 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.333 kb on + strand, within btuF at 178.440 kb on + strand at 178.440 kb on + strand at 178.448 kb on - strand at 178.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TN_18CS_18C_5dbl_rep2 remove 176,017 - clcA b0155 0.64 +0.1 176,017 - clcA b0155 0.64 -0.1 176,136 + clcA b0155 0.72 +0.0 176,173 + clcA b0155 0.75 -0.2 176,210 - clcA b0155 0.78 -0.7 176,210 - clcA b0155 0.78 -0.2 176,295 + clcA b0155 0.84 -0.8 176,356 + clcA b0155 0.88 -1.3 176,441 - +0.4 176,503 - +0.0 176,503 - -0.6 176,514 + +0.2 176,540 + +0.2 177,002 - -0.4 177,019 - +0.3 177,019 - +0.1 177,084 - yadS b0157 0.13 -1.3 177,084 - yadS b0157 0.13 +0.1 177,084 - yadS b0157 0.13 -1.0 177,084 - yadS b0157 0.13 -1.4 177,084 - yadS b0157 0.13 -0.8 177,084 - yadS b0157 0.13 -0.8 177,221 - yadS b0157 0.35 -1.0 177,257 + yadS b0157 0.41 -0.1 177,300 - yadS b0157 0.48 -0.1 177,300 - yadS b0157 0.48 -0.8 177,333 + yadS b0157 0.53 -1.0 177,341 - yadS b0157 0.54 -0.2 177,531 + yadS b0157 0.85 -0.1 177,578 + -0.6 177,596 - -1.4 177,596 - -0.4 177,616 + -0.7 177,616 + -0.2 177,625 + -0.8 177,625 + -0.3 177,697 + -0.6 177,714 - +0.5 177,726 + -0.6 177,735 - +0.4 178,094 - btuF b0158 0.54 -0.1 178,094 - btuF b0158 0.54 +0.0 178,138 + btuF b0158 0.59 -0.1 178,138 - btuF b0158 0.59 -0.3 178,150 - btuF b0158 0.61 +0.1 178,150 - btuF b0158 0.61 +0.6 178,304 - btuF b0158 0.80 -0.2 178,304 - btuF b0158 0.80 +0.2 178,333 + btuF b0158 0.84 -0.5 178,440 + +0.4 178,440 + +0.2 178,448 - +0.1 178,452 + +0.3
Or see this region's nucleotide sequence