Strain Fitness in Escherichia coli BW25113 around b1316

Experiment: TN_18CS_18C_10dbl_rep1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjS and ycjT overlap by 4 nucleotidesycjT and ycjU overlap by 4 nucleotidesycjU and ycjV are separated by 13 nucleotides b1315: ycjS - predicted oxidoreductase, NADH-binding (NCBI), at 1,374,856 to 1,375,911 ycjS b1316: ycjT - predicted hydrolase (NCBI), at 1,375,908 to 1,378,175 ycjT b1317: ycjU - predicted beta-phosphoglucomutase (NCBI), at 1,378,172 to 1,378,831 ycjU b4524: ycjV - no description, at 1,378,845 to 1,379,926 ycjV Position (kb) 1375 1376 1377 1378 1379Strain fitness (log2 ratio) -2 -1 0 1at 1375.200 kb on + strand, within ycjSat 1375.420 kb on + strand, within ycjSat 1375.426 kb on - strand, within ycjSat 1375.566 kb on + strand, within ycjSat 1375.566 kb on + strand, within ycjSat 1375.566 kb on + strand, within ycjSat 1375.701 kb on + strand, within ycjSat 1375.709 kb on - strand, within ycjSat 1375.709 kb on - strand, within ycjSat 1375.842 kb on - strandat 1375.872 kb on + strandat 1375.872 kb on + strandat 1375.985 kb on + strandat 1375.985 kb on + strandat 1376.172 kb on + strand, within ycjTat 1376.392 kb on - strand, within ycjTat 1376.562 kb on - strand, within ycjTat 1376.589 kb on - strand, within ycjTat 1376.589 kb on - strand, within ycjTat 1377.174 kb on - strand, within ycjTat 1377.634 kb on + strand, within ycjTat 1377.666 kb on - strand, within ycjTat 1377.676 kb on - strand, within ycjTat 1377.844 kb on + strand, within ycjTat 1377.850 kb on - strand, within ycjTat 1377.854 kb on + strand, within ycjTat 1377.967 kb on + strandat 1378.082 kb on + strandat 1378.082 kb on + strandat 1378.093 kb on + strandat 1378.093 kb on + strandat 1378.273 kb on - strand, within ycjUat 1378.326 kb on - strand, within ycjUat 1378.659 kb on - strand, within ycjUat 1378.707 kb on + strand, within ycjUat 1378.711 kb on - strand, within ycjUat 1378.887 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TN_18CS_18C_10dbl_rep1
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1,375,200 + ycjS b1315 0.33 -0.2
1,375,420 + ycjS b1315 0.53 +0.3
1,375,426 - ycjS b1315 0.54 -0.1
1,375,566 + ycjS b1315 0.67 +1.1
1,375,566 + ycjS b1315 0.67 +0.4
1,375,566 + ycjS b1315 0.67 -0.6
1,375,701 + ycjS b1315 0.80 +0.3
1,375,709 - ycjS b1315 0.81 -2.3
1,375,709 - ycjS b1315 0.81 +0.0
1,375,842 - +0.3
1,375,872 + +0.4
1,375,872 + +1.0
1,375,985 + +0.1
1,375,985 + +0.4
1,376,172 + ycjT b1316 0.12 +0.1
1,376,392 - ycjT b1316 0.21 +0.3
1,376,562 - ycjT b1316 0.29 +0.5
1,376,589 - ycjT b1316 0.30 -0.4
1,376,589 - ycjT b1316 0.30 +0.3
1,377,174 - ycjT b1316 0.56 -0.4
1,377,634 + ycjT b1316 0.76 -0.0
1,377,666 - ycjT b1316 0.78 +0.0
1,377,676 - ycjT b1316 0.78 +0.2
1,377,844 + ycjT b1316 0.85 -0.1
1,377,850 - ycjT b1316 0.86 +0.1
1,377,854 + ycjT b1316 0.86 -0.2
1,377,967 + -0.2
1,378,082 + +0.0
1,378,082 + +0.5
1,378,093 + +0.6
1,378,093 + +0.8
1,378,273 - ycjU b1317 0.15 -0.0
1,378,326 - ycjU b1317 0.23 -0.0
1,378,659 - ycjU b1317 0.74 +0.2
1,378,707 + ycjU b1317 0.81 -0.0
1,378,711 - ycjU b1317 0.82 +0.3
1,378,887 + +0.2

Or see this region's nucleotide sequence