Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12430 and ABIE41_RS12435 are separated by 16 nucleotides ABIE41_RS12435 and ABIE41_RS12440 are separated by 85 nucleotides ABIE41_RS12440 and ABIE41_RS12445 are separated by 178 nucleotides
ABIE41_RS12430: ABIE41_RS12430 - tripartite tricarboxylate transporter substrate-binding protein, at 2,544,682 to 2,545,638
_RS12430
ABIE41_RS12435: ABIE41_RS12435 - alpha-hydroxy acid oxidase, at 2,545,655 to 2,546,833
_RS12435
ABIE41_RS12440: ABIE41_RS12440 - YitT family protein, at 2,546,919 to 2,547,548
_RS12440
ABIE41_RS12445: ABIE41_RS12445 - glycoside hydrolase family 3 N-terminal domain-containing protein, at 2,547,727 to 2,549,961
_RS12445
Position (kb)
2545
2546
2547 Strain fitness (log2 ratio)
-2
-1
0
1 at 2544.717 kb on + strand at 2544.717 kb on + strand at 2544.717 kb on + strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.997 kb on + strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.207 kb on + strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.216 kb on + strand, within ABIE41_RS12430 at 2545.354 kb on - strand, within ABIE41_RS12430 at 2545.402 kb on + strand, within ABIE41_RS12430 at 2545.403 kb on - strand, within ABIE41_RS12430 at 2545.852 kb on - strand, within ABIE41_RS12435 at 2546.445 kb on + strand, within ABIE41_RS12435 at 2546.445 kb on + strand, within ABIE41_RS12435 at 2546.445 kb on + strand, within ABIE41_RS12435 at 2546.445 kb on + strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.446 kb on - strand, within ABIE41_RS12435 at 2546.727 kb on + strand at 2546.728 kb on - strand at 2546.760 kb on + strand at 2546.761 kb on - strand at 2546.767 kb on - strand at 2546.933 kb on + strand at 2546.933 kb on + strand at 2546.933 kb on + strand at 2546.934 kb on - strand at 2546.934 kb on - strand at 2546.934 kb on - strand at 2547.103 kb on + strand, within ABIE41_RS12440 at 2547.103 kb on + strand, within ABIE41_RS12440 at 2547.104 kb on - strand, within ABIE41_RS12440 at 2547.104 kb on - strand, within ABIE41_RS12440 at 2547.199 kb on + strand, within ABIE41_RS12440 at 2547.200 kb on - strand, within ABIE41_RS12440 at 2547.200 kb on - strand, within ABIE41_RS12440 at 2547.325 kb on + strand, within ABIE41_RS12440 at 2547.326 kb on - strand, within ABIE41_RS12440 at 2547.379 kb on + strand, within ABIE41_RS12440 at 2547.380 kb on - strand, within ABIE41_RS12440 at 2547.497 kb on - strand at 2547.497 kb on - strand at 2547.654 kb on + strand at 2547.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,544,717 + -0.1 2,544,717 + +0.7 2,544,717 + +0.2 2,544,718 - -1.4 2,544,718 - +0.6 2,544,718 - -0.2 2,544,718 - +0.4 2,544,997 + ABIE41_RS12430 0.33 +0.3 2,545,106 - ABIE41_RS12430 0.44 -0.3 2,545,106 - ABIE41_RS12430 0.44 -0.0 2,545,106 - ABIE41_RS12430 0.44 -0.4 2,545,207 + ABIE41_RS12430 0.55 +0.4 2,545,208 - ABIE41_RS12430 0.55 -0.1 2,545,208 - ABIE41_RS12430 0.55 +0.1 2,545,208 - ABIE41_RS12430 0.55 +0.2 2,545,208 - ABIE41_RS12430 0.55 +0.1 2,545,208 - ABIE41_RS12430 0.55 +0.4 2,545,216 + ABIE41_RS12430 0.56 +0.3 2,545,354 - ABIE41_RS12430 0.70 +0.6 2,545,402 + ABIE41_RS12430 0.75 +0.4 2,545,403 - ABIE41_RS12430 0.75 -0.2 2,545,852 - ABIE41_RS12435 0.17 -0.1 2,546,445 + ABIE41_RS12435 0.67 -1.8 2,546,445 + ABIE41_RS12435 0.67 -0.6 2,546,445 + ABIE41_RS12435 0.67 +0.4 2,546,445 + ABIE41_RS12435 0.67 -0.3 2,546,446 - ABIE41_RS12435 0.67 -0.2 2,546,446 - ABIE41_RS12435 0.67 +0.1 2,546,446 - ABIE41_RS12435 0.67 -0.4 2,546,446 - ABIE41_RS12435 0.67 -0.5 2,546,446 - ABIE41_RS12435 0.67 -0.9 2,546,446 - ABIE41_RS12435 0.67 +0.1 2,546,446 - ABIE41_RS12435 0.67 -0.8 2,546,727 + +0.1 2,546,728 - +0.1 2,546,760 + -0.6 2,546,761 - -1.1 2,546,767 - +0.1 2,546,933 + -1.4 2,546,933 + -0.9 2,546,933 + +0.9 2,546,934 - -0.3 2,546,934 - -1.4 2,546,934 - +0.5 2,547,103 + ABIE41_RS12440 0.29 -0.1 2,547,103 + ABIE41_RS12440 0.29 -1.1 2,547,104 - ABIE41_RS12440 0.29 +0.1 2,547,104 - ABIE41_RS12440 0.29 -0.1 2,547,199 + ABIE41_RS12440 0.44 -0.9 2,547,200 - ABIE41_RS12440 0.45 -0.8 2,547,200 - ABIE41_RS12440 0.45 -0.7 2,547,325 + ABIE41_RS12440 0.64 -1.3 2,547,326 - ABIE41_RS12440 0.65 -0.2 2,547,379 + ABIE41_RS12440 0.73 -0.8 2,547,380 - ABIE41_RS12440 0.73 -0.6 2,547,497 - -1.2 2,547,497 - -1.0 2,547,654 + -0.4 2,547,655 - -2.4
Or see this region's nucleotide sequence