Experiment: mixed (C)s Trisodium citrate 10 mM and 4-benzoyl-L-phenylalanine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_04769 and PFR28_04770 are separated by 88 nucleotides PFR28_04770 and PFR28_04771 are separated by 31 nucleotides PFR28_04771 and PFR28_04772 are separated by 100 nucleotides
PFR28_04769: PFR28_04769 - hypothetical protein, at 5,430,049 to 5,430,573
_04769
PFR28_04770: PFR28_04770 - CRP-like cAMP-activated global transcriptional regulator, at 5,430,662 to 5,431,348
_04770
PFR28_04771: PFR28_04771 - putative HTH-type transcriptional regulator, at 5,431,380 to 5,432,384
_04771
PFR28_04772: PFR28_04772 - hypothetical protein, at 5,432,485 to 5,433,558
_04772
Position (kb) 5431
5432
5433 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5430.380 kb on + strand, within PFR28_04769 at 5430.381 kb on - strand, within PFR28_04769 at 5430.533 kb on + strand at 5430.533 kb on + strand at 5430.533 kb on + strand at 5430.533 kb on + strand at 5430.533 kb on + strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.534 kb on - strand at 5430.542 kb on + strand at 5430.543 kb on - strand at 5430.543 kb on - strand at 5430.636 kb on + strand at 5430.684 kb on + strand at 5430.684 kb on + strand at 5430.685 kb on - strand at 5430.685 kb on - strand at 5430.685 kb on - strand at 5430.707 kb on + strand at 5430.707 kb on + strand at 5430.729 kb on + strand at 5430.730 kb on - strand at 5430.730 kb on - strand at 5430.737 kb on - strand, within PFR28_04770 at 5430.737 kb on - strand, within PFR28_04770 at 5430.818 kb on + strand, within PFR28_04770 at 5430.818 kb on + strand, within PFR28_04770 at 5430.819 kb on - strand, within PFR28_04770 at 5430.819 kb on - strand, within PFR28_04770 at 5431.032 kb on - strand, within PFR28_04770 at 5431.221 kb on + strand, within PFR28_04770 at 5431.222 kb on - strand, within PFR28_04770 at 5431.892 kb on - strand, within PFR28_04771 at 5431.892 kb on - strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.945 kb on + strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5431.946 kb on - strand, within PFR28_04771 at 5432.077 kb on + strand, within PFR28_04771 at 5432.209 kb on - strand, within PFR28_04771 at 5432.419 kb on + strand at 5432.428 kb on + strand at 5432.428 kb on + strand at 5432.428 kb on + strand at 5432.429 kb on - strand at 5432.429 kb on - strand at 5432.429 kb on - strand at 5432.429 kb on - strand at 5432.429 kb on - strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.479 kb on + strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.480 kb on - strand at 5432.576 kb on + strand at 5432.577 kb on - strand at 5432.577 kb on - strand at 5432.577 kb on - strand at 5432.671 kb on + strand, within PFR28_04772 at 5432.671 kb on + strand, within PFR28_04772 at 5432.672 kb on - strand, within PFR28_04772 at 5432.672 kb on - strand, within PFR28_04772 at 5432.672 kb on - strand, within PFR28_04772 at 5432.672 kb on - strand, within PFR28_04772 at 5432.672 kb on - strand, within PFR28_04772 at 5432.673 kb on + strand, within PFR28_04772 at 5432.673 kb on + strand, within PFR28_04772 at 5432.770 kb on + strand, within PFR28_04772 at 5432.771 kb on - strand, within PFR28_04772 at 5432.885 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.055 kb on + strand, within PFR28_04772 at 5433.056 kb on - strand, within PFR28_04772 at 5433.056 kb on - strand, within PFR28_04772 at 5433.056 kb on - strand, within PFR28_04772 at 5433.056 kb on - strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.091 kb on + strand, within PFR28_04772 at 5433.092 kb on - strand, within PFR28_04772 at 5433.092 kb on - strand, within PFR28_04772 at 5433.092 kb on - strand, within PFR28_04772 at 5433.092 kb on - strand, within PFR28_04772 at 5433.141 kb on + strand, within PFR28_04772 at 5433.141 kb on + strand, within PFR28_04772 at 5433.142 kb on - strand, within PFR28_04772 at 5433.224 kb on + strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.225 kb on - strand, within PFR28_04772 at 5433.231 kb on + strand, within PFR28_04772 at 5433.231 kb on + strand, within PFR28_04772 at 5433.232 kb on - strand, within PFR28_04772 at 5433.269 kb on - strand, within PFR28_04772 at 5433.269 kb on - strand, within PFR28_04772 at 5433.269 kb on - strand, within PFR28_04772 at 5433.269 kb on - strand, within PFR28_04772 at 5433.272 kb on - strand, within PFR28_04772 at 5433.369 kb on + strand, within PFR28_04772 at 5433.370 kb on - strand, within PFR28_04772 at 5433.370 kb on - strand, within PFR28_04772 at 5433.370 kb on - strand, within PFR28_04772
Per-strain Table
Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 4-benzoyl-L-phenylalanine 5 mM remove 5,430,380 + PFR28_04769 0.63 +0.2 5,430,381 - PFR28_04769 0.63 +0.6 5,430,533 + +0.4 5,430,533 + -1.0 5,430,533 + -0.5 5,430,533 + -1.6 5,430,533 + -0.2 5,430,534 - -1.3 5,430,534 - -0.6 5,430,534 - +0.8 5,430,534 - -0.9 5,430,534 - -0.4 5,430,534 - -1.0 5,430,534 - +1.1 5,430,542 + -0.3 5,430,543 - -0.5 5,430,543 - -0.0 5,430,636 + -1.0 5,430,684 + +0.1 5,430,684 + +0.0 5,430,685 - +0.5 5,430,685 - +0.0 5,430,685 - -0.4 5,430,707 + -0.5 5,430,707 + +1.1 5,430,729 + +0.1 5,430,730 - +0.8 5,430,730 - -0.1 5,430,737 - PFR28_04770 0.11 -0.2 5,430,737 - PFR28_04770 0.11 -0.8 5,430,818 + PFR28_04770 0.23 -0.9 5,430,818 + PFR28_04770 0.23 -0.7 5,430,819 - PFR28_04770 0.23 -0.7 5,430,819 - PFR28_04770 0.23 -0.1 5,431,032 - PFR28_04770 0.54 +0.0 5,431,221 + PFR28_04770 0.81 +1.0 5,431,222 - PFR28_04770 0.82 -0.4 5,431,892 - PFR28_04771 0.51 +1.4 5,431,892 - PFR28_04771 0.51 +0.0 5,431,945 + PFR28_04771 0.56 -0.6 5,431,945 + PFR28_04771 0.56 -1.1 5,431,945 + PFR28_04771 0.56 +0.2 5,431,945 + PFR28_04771 0.56 +0.1 5,431,945 + PFR28_04771 0.56 +0.7 5,431,945 + PFR28_04771 0.56 -0.5 5,431,945 + PFR28_04771 0.56 -1.1 5,431,945 + PFR28_04771 0.56 -0.2 5,431,946 - PFR28_04771 0.56 -0.8 5,431,946 - PFR28_04771 0.56 +0.2 5,431,946 - PFR28_04771 0.56 +0.7 5,431,946 - PFR28_04771 0.56 +0.1 5,431,946 - PFR28_04771 0.56 +1.4 5,431,946 - PFR28_04771 0.56 -0.4 5,431,946 - PFR28_04771 0.56 +0.3 5,431,946 - PFR28_04771 0.56 -0.3 5,432,077 + PFR28_04771 0.69 +0.0 5,432,209 - PFR28_04771 0.82 -0.0 5,432,419 + +0.4 5,432,428 + +0.8 5,432,428 + +0.1 5,432,428 + -0.8 5,432,429 - +0.3 5,432,429 - +0.2 5,432,429 - -0.6 5,432,429 - +0.3 5,432,429 - -1.5 5,432,479 + -0.2 5,432,479 + -0.4 5,432,479 + +1.5 5,432,479 + +0.3 5,432,479 + -0.4 5,432,479 + +0.0 5,432,479 + +1.1 5,432,479 + -1.0 5,432,479 + +0.9 5,432,480 - +0.2 5,432,480 - +0.0 5,432,480 - -0.2 5,432,480 - -0.1 5,432,480 - -3.1 5,432,480 - -0.3 5,432,480 - +0.2 5,432,480 - -1.0 5,432,480 - -0.3 5,432,576 + -0.0 5,432,577 - -0.2 5,432,577 - +2.0 5,432,577 - +0.0 5,432,671 + PFR28_04772 0.17 +0.4 5,432,671 + PFR28_04772 0.17 -1.5 5,432,672 - PFR28_04772 0.17 +0.1 5,432,672 - PFR28_04772 0.17 +0.2 5,432,672 - PFR28_04772 0.17 +0.2 5,432,672 - PFR28_04772 0.17 +0.6 5,432,672 - PFR28_04772 0.17 +0.4 5,432,673 + PFR28_04772 0.18 +0.0 5,432,673 + PFR28_04772 0.18 -0.2 5,432,770 + PFR28_04772 0.27 +0.2 5,432,771 - PFR28_04772 0.27 -0.0 5,432,885 + PFR28_04772 0.37 +0.4 5,433,055 + PFR28_04772 0.53 +0.7 5,433,055 + PFR28_04772 0.53 -0.4 5,433,055 + PFR28_04772 0.53 +0.3 5,433,055 + PFR28_04772 0.53 +0.2 5,433,055 + PFR28_04772 0.53 +0.4 5,433,055 + PFR28_04772 0.53 +0.7 5,433,056 - PFR28_04772 0.53 -0.2 5,433,056 - PFR28_04772 0.53 +1.0 5,433,056 - PFR28_04772 0.53 -1.0 5,433,056 - PFR28_04772 0.53 -0.4 5,433,091 + PFR28_04772 0.56 -0.8 5,433,091 + PFR28_04772 0.56 +0.1 5,433,091 + PFR28_04772 0.56 -0.1 5,433,091 + PFR28_04772 0.56 -0.3 5,433,091 + PFR28_04772 0.56 +0.2 5,433,091 + PFR28_04772 0.56 -0.1 5,433,092 - PFR28_04772 0.57 +0.3 5,433,092 - PFR28_04772 0.57 -0.7 5,433,092 - PFR28_04772 0.57 +0.1 5,433,092 - PFR28_04772 0.57 -1.5 5,433,141 + PFR28_04772 0.61 -0.6 5,433,141 + PFR28_04772 0.61 +0.6 5,433,142 - PFR28_04772 0.61 -0.5 5,433,224 + PFR28_04772 0.69 -0.5 5,433,225 - PFR28_04772 0.69 +0.4 5,433,225 - PFR28_04772 0.69 +2.8 5,433,225 - PFR28_04772 0.69 +0.0 5,433,225 - PFR28_04772 0.69 +0.5 5,433,225 - PFR28_04772 0.69 +0.5 5,433,225 - PFR28_04772 0.69 -0.1 5,433,225 - PFR28_04772 0.69 +0.2 5,433,231 + PFR28_04772 0.69 +1.7 5,433,231 + PFR28_04772 0.69 +0.4 5,433,232 - PFR28_04772 0.70 +0.8 5,433,269 - PFR28_04772 0.73 +0.1 5,433,269 - PFR28_04772 0.73 -0.8 5,433,269 - PFR28_04772 0.73 +0.0 5,433,269 - PFR28_04772 0.73 +2.1 5,433,272 - PFR28_04772 0.73 -0.4 5,433,369 + PFR28_04772 0.82 -0.2 5,433,370 - PFR28_04772 0.82 -0.2 5,433,370 - PFR28_04772 0.82 +0.2 5,433,370 - PFR28_04772 0.82 +0.6
Or see this region's nucleotide sequence