Experiment: Matrix:KB soft agar;Phage:PBS;Temp:28;Samp:32-3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OLJFJH_04075 and OLJFJH_04080 are separated by 37 nucleotides OLJFJH_04080 and OLJFJH_04085 are separated by 119 nucleotides OLJFJH_04085 and OLJFJH_04090 are separated by 91 nucleotides
OLJFJH_04075: OLJFJH_04075 - ribulose-phosphate 3-epimerase, at 774,554 to 775,240
_04075
OLJFJH_04080: OLJFJH_04080 - adenine-specific DNA-methyltransferase, at 775,278 to 776,099
_04080
OLJFJH_04085: OLJFJH_04085 - cell division protein DamX, at 776,219 to 777,175
_04085
OLJFJH_04090: OLJFJH_04090 - 3-dehydroquinate synthase, at 777,267 to 778,355
_04090
Position (kb) 776
777
778 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 775.292 kb on - strand at 775.292 kb on - strand at 775.292 kb on - strand at 775.292 kb on - strand at 775.434 kb on - strand, within OLJFJH_04080 at 775.675 kb on + strand, within OLJFJH_04080 at 775.744 kb on + strand, within OLJFJH_04080 at 775.745 kb on - strand, within OLJFJH_04080 at 775.745 kb on - strand, within OLJFJH_04080 at 775.757 kb on + strand, within OLJFJH_04080 at 775.763 kb on - strand, within OLJFJH_04080 at 775.764 kb on + strand, within OLJFJH_04080 at 775.825 kb on + strand, within OLJFJH_04080 at 775.825 kb on + strand, within OLJFJH_04080 at 775.829 kb on - strand, within OLJFJH_04080 at 775.829 kb on - strand, within OLJFJH_04080 at 775.829 kb on - strand, within OLJFJH_04080 at 775.949 kb on + strand, within OLJFJH_04080 at 775.951 kb on + strand, within OLJFJH_04080 at 775.952 kb on - strand, within OLJFJH_04080 at 776.057 kb on - strand at 776.060 kb on + strand at 776.060 kb on + strand at 776.061 kb on - strand at 776.061 kb on - strand at 776.061 kb on - strand at 776.061 kb on - strand at 776.061 kb on - strand at 776.112 kb on + strand at 776.112 kb on + strand at 776.112 kb on + strand at 776.113 kb on - strand at 776.113 kb on - strand at 776.113 kb on - strand at 776.113 kb on - strand at 776.113 kb on - strand at 776.113 kb on - strand at 776.117 kb on - strand at 776.117 kb on - strand at 776.117 kb on - strand at 776.117 kb on - strand at 776.147 kb on + strand at 776.147 kb on + strand at 776.147 kb on + strand at 776.147 kb on + strand at 776.148 kb on - strand at 776.148 kb on - strand at 776.148 kb on - strand at 776.159 kb on - strand at 776.220 kb on + strand at 776.279 kb on + strand at 776.279 kb on + strand at 776.279 kb on + strand at 776.279 kb on + strand at 776.331 kb on + strand, within OLJFJH_04085 at 776.352 kb on + strand, within OLJFJH_04085 at 776.353 kb on - strand, within OLJFJH_04085 at 776.353 kb on - strand, within OLJFJH_04085 at 776.353 kb on - strand, within OLJFJH_04085 at 776.353 kb on - strand, within OLJFJH_04085 at 776.383 kb on - strand, within OLJFJH_04085 at 776.483 kb on - strand, within OLJFJH_04085 at 776.483 kb on - strand, within OLJFJH_04085 at 776.832 kb on - strand, within OLJFJH_04085 at 776.941 kb on + strand, within OLJFJH_04085 at 777.064 kb on + strand, within OLJFJH_04085 at 777.065 kb on - strand, within OLJFJH_04085 at 777.065 kb on - strand, within OLJFJH_04085 at 777.065 kb on - strand, within OLJFJH_04085 at 777.106 kb on + strand at 777.107 kb on - strand at 777.146 kb on + strand at 777.178 kb on - strand at 777.178 kb on - strand at 777.218 kb on - strand at 777.228 kb on + strand at 777.228 kb on + strand at 777.228 kb on + strand at 777.228 kb on + strand at 777.229 kb on - strand at 777.229 kb on - strand at 777.229 kb on - strand at 777.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Matrix:KB soft agar;Phage:PBS;Temp:28;Samp:32-3 remove 775,292 - +0.7 775,292 - +1.3 775,292 - -0.5 775,292 - +2.3 775,434 - OLJFJH_04080 0.19 +0.7 775,675 + OLJFJH_04080 0.48 +0.3 775,744 + OLJFJH_04080 0.57 -0.0 775,745 - OLJFJH_04080 0.57 +0.7 775,745 - OLJFJH_04080 0.57 +1.3 775,757 + OLJFJH_04080 0.58 -0.3 775,763 - OLJFJH_04080 0.59 +0.3 775,764 + OLJFJH_04080 0.59 +2.3 775,825 + OLJFJH_04080 0.67 +2.3 775,825 + OLJFJH_04080 0.67 +2.3 775,829 - OLJFJH_04080 0.67 +2.3 775,829 - OLJFJH_04080 0.67 +0.7 775,829 - OLJFJH_04080 0.67 +1.3 775,949 + OLJFJH_04080 0.82 +1.3 775,951 + OLJFJH_04080 0.82 +0.7 775,952 - OLJFJH_04080 0.82 +1.3 776,057 - +1.3 776,060 + +0.7 776,060 + +3.3 776,061 - +1.3 776,061 - -0.0 776,061 - +1.3 776,061 - -1.2 776,061 - +0.7 776,112 + +1.3 776,112 + -0.5 776,112 + +2.3 776,113 - +0.5 776,113 - +0.8 776,113 - -0.0 776,113 - +0.6 776,113 - -0.7 776,113 - -0.5 776,117 - +0.3 776,117 - +0.0 776,117 - +0.0 776,117 - -0.4 776,147 + -0.3 776,147 + +1.3 776,147 + +0.7 776,147 + +2.3 776,148 - -2.9 776,148 - -0.2 776,148 - -0.6 776,159 - -1.2 776,220 + +0.3 776,279 + -0.0 776,279 + -0.0 776,279 + -0.7 776,279 + -0.0 776,331 + OLJFJH_04085 0.12 +0.3 776,352 + OLJFJH_04085 0.14 -0.0 776,353 - OLJFJH_04085 0.14 -0.3 776,353 - OLJFJH_04085 0.14 -1.5 776,353 - OLJFJH_04085 0.14 -2.0 776,353 - OLJFJH_04085 0.14 -1.0 776,383 - OLJFJH_04085 0.17 -1.2 776,483 - OLJFJH_04085 0.28 -0.3 776,483 - OLJFJH_04085 0.28 -1.6 776,832 - OLJFJH_04085 0.64 -3.1 776,941 + OLJFJH_04085 0.75 -0.0 777,064 + OLJFJH_04085 0.88 +0.3 777,065 - OLJFJH_04085 0.88 -0.9 777,065 - OLJFJH_04085 0.88 +0.3 777,065 - OLJFJH_04085 0.88 +0.7 777,106 + +1.3 777,107 - -0.3 777,146 + +0.3 777,178 - -0.9 777,178 - +2.3 777,218 - -0.6 777,228 + +2.3 777,228 + +2.3 777,228 + +0.7 777,228 + +0.7 777,229 - -1.2 777,229 - -0.3 777,229 - +0.3 777,266 - -0.5
Or see this region's nucleotide sequence