Strain Fitness in Escherichia coli BW25113 around b0051

Experiment: M63 no sulfur + 5 mM sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntapaH and apaG are separated by 6 nucleotidesapaG and ksgA are separated by 2 nucleotidesksgA and pdxA overlap by 4 nucleotidespdxA and surA overlap by 1 nucleotides b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606 apaG b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430 ksgA b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416 pdxA b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702 surA Position (kb) 51 52 53Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 50.717 kb on - strand, within apaHat 50.785 kb on - strand, within apaHat 50.791 kb on + strand, within apaHat 50.802 kb on + strand, within apaHat 50.829 kb on - strand, within apaHat 50.859 kb on - strand, within apaHat 50.877 kb on + strand, within apaHat 50.877 kb on + strand, within apaHat 51.025 kb on + strand, within apaHat 51.109 kb on - strand, within apaHat 51.177 kb on + strandat 51.177 kb on + strandat 51.185 kb on - strandat 51.190 kb on + strandat 51.190 kb on + strandat 51.240 kb on + strandat 51.240 kb on + strandat 51.311 kb on + strand, within apaGat 51.311 kb on + strand, within apaGat 51.322 kb on - strand, within apaGat 51.326 kb on - strand, within apaGat 51.334 kb on - strand, within apaGat 51.336 kb on - strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on - strand, within apaGat 51.364 kb on - strand, within apaGat 51.372 kb on - strand, within apaGat 51.415 kb on + strand, within apaGat 51.423 kb on - strand, within apaGat 51.466 kb on + strand, within apaGat 51.472 kb on + strand, within apaGat 51.512 kb on - strand, within apaGat 51.514 kb on - strand, within apaGat 51.520 kb on - strand, within apaGat 51.524 kb on + strand, within apaGat 51.550 kb on - strand, within apaGat 51.560 kb on + strand, within apaGat 51.567 kb on - strand, within apaGat 51.567 kb on - strand, within apaGat 51.576 kb on + strandat 51.576 kb on + strandat 51.586 kb on - strandat 51.613 kb on + strandat 51.614 kb on - strandat 51.615 kb on + strandat 51.615 kb on + strandat 51.673 kb on - strandat 51.703 kb on + strand, within ksgAat 51.703 kb on + strand, within ksgAat 51.812 kb on - strand, within ksgAat 51.836 kb on + strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.904 kb on + strand, within ksgAat 51.906 kb on + strand, within ksgAat 51.925 kb on - strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.045 kb on + strand, within ksgAat 52.062 kb on + strand, within ksgAat 52.062 kb on + strandat 52.070 kb on - strand, within ksgAat 52.072 kb on + strand, within ksgAat 52.089 kb on - strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.284 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.343 kb on - strand, within ksgAat 52.348 kb on - strandat 52.348 kb on - strandat 52.348 kb on - strandat 52.354 kb on + strandat 52.420 kb on - strandat 52.490 kb on + strandat 52.490 kb on + strandat 52.502 kb on + strandat 52.502 kb on + strandat 52.518 kb on - strandat 52.518 kb on - strandat 52.626 kb on + strand, within pdxAat 52.688 kb on - strand, within pdxAat 52.735 kb on + strand, within pdxAat 52.735 kb on + strand, within pdxAat 52.740 kb on - strand, within pdxAat 52.766 kb on - strand, within pdxAat 52.780 kb on - strand, within pdxAat 52.797 kb on - strand, within pdxAat 52.879 kb on - strand, within pdxAat 52.914 kb on + strand, within pdxAat 52.982 kb on - strand, within pdxAat 52.982 kb on - strand, within pdxAat 52.983 kb on + strand, within pdxAat 53.061 kb on - strand, within pdxAat 53.062 kb on - strand, within pdxAat 53.062 kb on - strandat 53.064 kb on + strand, within pdxAat 53.067 kb on - strand, within pdxAat 53.163 kb on - strand, within pdxAat 53.163 kb on - strand, within pdxAat 53.181 kb on - strand, within pdxAat 53.181 kb on - strand, within pdxAat 53.202 kb on - strand, within pdxAat 53.221 kb on + strand, within pdxAat 53.221 kb on + strand, within pdxAat 53.226 kb on - strand, within pdxAat 53.246 kb on - strand, within pdxAat 53.291 kb on + strand, within pdxAat 53.328 kb on + strandat 53.328 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.346 kb on - strandat 53.346 kb on - strandat 53.346 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63 no sulfur + 5 mM sulfate
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50,717 - apaH b0049 0.40 -4.6
50,785 - apaH b0049 0.48 -5.2
50,791 + apaH b0049 0.49 -2.7
50,802 + apaH b0049 0.50 -4.7
50,829 - apaH b0049 0.53 -1.8
50,859 - apaH b0049 0.57 -4.6
50,877 + apaH b0049 0.59 -1.1
50,877 + apaH b0049 0.59 -2.1
51,025 + apaH b0049 0.77 -4.7
51,109 - apaH b0049 0.86 -5.2
51,177 + -3.5
51,177 + -3.1
51,185 - -4.8
51,190 + -2.6
51,190 + -4.3
51,240 + -0.9
51,240 + +0.6
51,311 + apaG b0050 0.22 +0.2
51,311 + apaG b0050 0.22 -0.6
51,322 - apaG b0050 0.25 +0.2
51,326 - apaG b0050 0.26 +0.7
51,334 - apaG b0050 0.28 +0.6
51,336 - apaG b0050 0.28 +0.3
51,353 + apaG b0050 0.33 -0.0
51,353 + apaG b0050 0.33 -0.2
51,353 - apaG b0050 0.33 +0.7
51,364 - apaG b0050 0.36 -0.6
51,372 - apaG b0050 0.38 +0.2
51,415 + apaG b0050 0.49 +0.2
51,423 - apaG b0050 0.51 +0.4
51,466 + apaG b0050 0.63 +0.1
51,472 + apaG b0050 0.64 +0.2
51,512 - apaG b0050 0.75 -0.3
51,514 - apaG b0050 0.75 +0.3
51,520 - apaG b0050 0.77 +0.2
51,524 + apaG b0050 0.78 -1.2
51,550 - apaG b0050 0.85 +0.5
51,560 + apaG b0050 0.88 -0.3
51,567 - apaG b0050 0.89 -0.5
51,567 - apaG b0050 0.89 -0.0
51,576 + -1.3
51,576 + -0.8
51,586 - +0.6
51,613 + +0.1
51,614 - -0.1
51,615 + +0.5
51,615 + -0.0
51,673 - -1.9
51,703 + ksgA b0051 0.11 -2.8
51,703 + ksgA b0051 0.11 -3.8
51,812 - ksgA b0051 0.25 -4.4
51,836 + ksgA b0051 0.28 -1.8
51,891 - ksgA b0051 0.34 -1.6
51,891 - ksgA b0051 0.34 -1.9
51,904 + ksgA b0051 0.36 -2.8
51,906 + ksgA b0051 0.36 +0.6
51,925 - ksgA b0051 0.38 -3.9
52,040 + ksgA b0051 0.52 -3.1
52,040 + ksgA b0051 0.52 -3.5
52,040 + ksgA b0051 0.52 -2.5
52,042 + ksgA b0051 0.53 -3.5
52,042 + ksgA b0051 0.53 -2.6
52,042 + ksgA b0051 0.53 -2.4
52,042 - ksgA b0051 0.53 -3.0
52,042 - ksgA b0051 0.53 -2.2
52,045 + ksgA b0051 0.53 -2.6
52,062 + ksgA b0051 0.55 -4.4
52,062 + -2.6
52,070 - ksgA b0051 0.56 -3.9
52,072 + ksgA b0051 0.56 -1.7
52,089 - ksgA b0051 0.58 -3.0
52,276 + ksgA b0051 0.81 -3.0
52,276 + ksgA b0051 0.81 -2.9
52,284 + ksgA b0051 0.82 -4.6
52,338 + ksgA b0051 0.89 -1.5
52,338 + ksgA b0051 0.89 -3.9
52,343 - ksgA b0051 0.89 -4.4
52,348 - -1.1
52,348 - -1.9
52,348 - -1.6
52,354 + -3.3
52,420 - -3.5
52,490 + -3.4
52,490 + -3.7
52,502 + -4.3
52,502 + -3.7
52,518 - -4.0
52,518 - -2.7
52,626 + pdxA b0052 0.20 -3.9
52,688 - pdxA b0052 0.26 -2.8
52,735 + pdxA b0052 0.31 -3.2
52,735 + pdxA b0052 0.31 -2.0
52,740 - pdxA b0052 0.32 -4.4
52,766 - pdxA b0052 0.34 -1.3
52,780 - pdxA b0052 0.36 -3.3
52,797 - pdxA b0052 0.37 -5.5
52,879 - pdxA b0052 0.46 -5.2
52,914 + pdxA b0052 0.49 -3.6
52,982 - pdxA b0052 0.56 -3.6
52,982 - pdxA b0052 0.56 -1.4
52,983 + pdxA b0052 0.56 -2.9
53,061 - pdxA b0052 0.64 -4.2
53,062 - pdxA b0052 0.64 -3.1
53,062 - -0.4
53,064 + pdxA b0052 0.64 -3.7
53,067 - pdxA b0052 0.65 -3.6
53,163 - pdxA b0052 0.74 -3.9
53,163 - pdxA b0052 0.74 -2.7
53,181 - pdxA b0052 0.76 -4.4
53,181 - pdxA b0052 0.76 -3.5
53,202 - pdxA b0052 0.78 -2.6
53,221 + pdxA b0052 0.80 -3.2
53,221 + pdxA b0052 0.80 -3.2
53,226 - pdxA b0052 0.81 -1.6
53,246 - pdxA b0052 0.83 -3.6
53,291 + pdxA b0052 0.87 -4.2
53,328 + -3.4
53,328 + -2.7
53,338 + -2.4
53,338 + -3.0
53,338 + -4.2
53,338 + -4.3
53,346 - -3.6
53,346 - -3.3
53,346 - -3.5

Or see this region's nucleotide sequence