Strain Fitness in Escherichia coli BW25113 around b1870

Experiment: M63 no sulfur + 5 mM sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecE and yecN are separated by 52 nucleotidesyecN and yecO are separated by 40 nucleotidesyecO and yecP overlap by 4 nucleotides b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237 yecE b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685 yecN b1870: yecO - predicted methyltransferase (NCBI), at 1,950,726 to 1,951,469 yecO b1871: yecP - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,951,466 to 1,952,437 yecP Position (kb) 1950 1951 1952Strain fitness (log2 ratio) -3 -2 -1 0 1at 1949.746 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.931 kb on + strand, within yecEat 1950.249 kb on + strandat 1950.310 kb on - strandat 1950.444 kb on + strand, within yecNat 1950.475 kb on + strand, within yecNat 1950.475 kb on + strand, within yecNat 1950.509 kb on + strand, within yecNat 1950.517 kb on - strand, within yecNat 1950.525 kb on + strand, within yecNat 1950.525 kb on + strand, within yecNat 1950.612 kb on + strand, within yecNat 1950.612 kb on + strand, within yecNat 1950.613 kb on - strand, within yecNat 1950.613 kb on - strand, within yecNat 1950.743 kb on + strandat 1951.029 kb on + strand, within yecOat 1951.519 kb on - strandat 1951.579 kb on + strand, within yecPat 1951.831 kb on - strand, within yecPat 1951.831 kb on - strand, within yecPat 1951.834 kb on + strand, within yecPat 1951.834 kb on + strand, within yecPat 1951.868 kb on + strand, within yecPat 1951.912 kb on + strand, within yecPat 1951.912 kb on + strand, within yecPat 1951.920 kb on + strand, within yecPat 1952.017 kb on - strand, within yecPat 1952.017 kb on - strand, within yecPat 1952.288 kb on - strand, within yecPat 1952.330 kb on - strand, within yecPat 1952.330 kb on - strand, within yecPat 1952.419 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63 no sulfur + 5 mM sulfate
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1,949,746 - yecE b1868 0.40 -0.5
1,949,842 - yecE b1868 0.52 +0.7
1,949,842 - yecE b1868 0.52 +0.4
1,949,931 + yecE b1868 0.63 -0.3
1,950,249 + -0.2
1,950,310 - +0.4
1,950,444 + yecN b1869 0.39 +0.1
1,950,475 + yecN b1869 0.47 +0.5
1,950,475 + yecN b1869 0.47 +0.0
1,950,509 + yecN b1869 0.55 -0.5
1,950,517 - yecN b1869 0.57 -0.7
1,950,525 + yecN b1869 0.59 -0.5
1,950,525 + yecN b1869 0.59 +0.1
1,950,612 + yecN b1869 0.81 +0.6
1,950,612 + yecN b1869 0.81 -0.3
1,950,613 - yecN b1869 0.82 -0.0
1,950,613 - yecN b1869 0.82 +0.2
1,950,743 + -0.3
1,951,029 + yecO b1870 0.41 -1.2
1,951,519 - -1.4
1,951,579 + yecP b1871 0.12 -0.8
1,951,831 - yecP b1871 0.38 -1.0
1,951,831 - yecP b1871 0.38 -2.0
1,951,834 + yecP b1871 0.38 -1.5
1,951,834 + yecP b1871 0.38 -1.5
1,951,868 + yecP b1871 0.41 -2.4
1,951,912 + yecP b1871 0.46 -1.7
1,951,912 + yecP b1871 0.46 +0.0
1,951,920 + yecP b1871 0.47 -2.8
1,952,017 - yecP b1871 0.57 -1.2
1,952,017 - yecP b1871 0.57 -1.7
1,952,288 - yecP b1871 0.85 -1.4
1,952,330 - yecP b1871 0.89 -1.8
1,952,330 - yecP b1871 0.89 -0.7
1,952,419 + +0.2

Or see this region's nucleotide sequence