Strain Fitness in Escherichia coli BW25113 around b3432

Experiment: M63 no sulfur + 5 mM L-cys

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglgX and glgB overlap by 4 nucleotidesglgB and asd are separated by 272 nucleotides b3431: glgX - glycogen debranching enzyme (NCBI), at 3,567,369 to 3,569,342 glgX b3432: glgB - glycogen branching enzyme (NCBI), at 3,569,339 to 3,571,525 glgB b3433: asd - aspartate-semialdehyde dehydrogenase (NCBI), at 3,571,798 to 3,572,901 asd Position (kb) 3569 3570 3571 3572Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3568.499 kb on - strand, within glgXat 3568.590 kb on - strand, within glgXat 3568.602 kb on - strand, within glgXat 3568.641 kb on + strand, within glgXat 3568.649 kb on - strand, within glgXat 3568.649 kb on - strand, within glgXat 3568.649 kb on - strand, within glgXat 3568.682 kb on + strand, within glgXat 3568.695 kb on + strand, within glgXat 3568.695 kb on + strand, within glgXat 3568.695 kb on + strand, within glgXat 3568.701 kb on + strand, within glgXat 3568.708 kb on - strand, within glgXat 3568.708 kb on - strand, within glgXat 3568.726 kb on + strand, within glgXat 3568.842 kb on - strand, within glgXat 3568.886 kb on + strand, within glgXat 3568.886 kb on + strand, within glgXat 3568.930 kb on + strand, within glgXat 3568.932 kb on + strand, within glgXat 3568.935 kb on + strand, within glgXat 3568.946 kb on - strand, within glgXat 3568.984 kb on + strand, within glgXat 3568.984 kb on + strand, within glgXat 3569.026 kb on + strand, within glgXat 3569.026 kb on + strand, within glgXat 3569.034 kb on - strand, within glgXat 3569.114 kb on + strand, within glgXat 3569.114 kb on + strand, within glgXat 3569.151 kb on - strandat 3569.151 kb on - strandat 3569.159 kb on + strandat 3569.159 kb on + strandat 3569.192 kb on - strandat 3569.192 kb on - strandat 3569.240 kb on + strandat 3569.240 kb on + strandat 3569.248 kb on - strandat 3569.248 kb on - strandat 3569.248 kb on - strandat 3569.277 kb on + strandat 3569.288 kb on + strandat 3569.328 kb on + strandat 3569.357 kb on - strandat 3569.357 kb on - strandat 3569.359 kb on + strandat 3569.359 kb on + strandat 3569.366 kb on - strandat 3569.389 kb on + strandat 3569.429 kb on + strandat 3569.429 kb on + strandat 3569.432 kb on + strandat 3569.432 kb on + strandat 3569.437 kb on - strandat 3569.437 kb on - strandat 3569.437 kb on - strandat 3569.437 kb on - strandat 3569.442 kb on - strandat 3569.442 kb on - strandat 3569.452 kb on + strandat 3569.460 kb on - strandat 3569.460 kb on - strandat 3569.460 kb on - strandat 3569.516 kb on + strandat 3569.523 kb on - strandat 3569.523 kb on - strandat 3569.541 kb on + strandat 3569.541 kb on + strandat 3569.556 kb on + strandat 3569.556 kb on + strandat 3569.635 kb on - strand, within glgBat 3569.667 kb on - strand, within glgBat 3569.702 kb on - strand, within glgBat 3569.723 kb on + strand, within glgBat 3569.779 kb on - strand, within glgBat 3569.813 kb on + strand, within glgBat 3569.828 kb on - strand, within glgBat 3569.896 kb on - strand, within glgBat 3569.903 kb on + strand, within glgBat 3569.960 kb on - strand, within glgBat 3569.960 kb on - strand, within glgBat 3569.984 kb on + strand, within glgBat 3569.984 kb on + strand, within glgBat 3569.984 kb on + strand, within glgBat 3570.002 kb on + strand, within glgBat 3570.002 kb on + strand, within glgBat 3570.007 kb on + strand, within glgBat 3570.011 kb on + strand, within glgBat 3570.014 kb on - strand, within glgBat 3570.014 kb on - strand, within glgBat 3570.015 kb on - strand, within glgBat 3570.015 kb on - strand, within glgBat 3570.061 kb on - strand, within glgBat 3570.061 kb on - strand, within glgBat 3570.062 kb on + strand, within glgBat 3570.097 kb on + strand, within glgBat 3570.105 kb on - strand, within glgBat 3570.115 kb on - strand, within glgBat 3570.116 kb on - strand, within glgBat 3570.231 kb on - strand, within glgBat 3570.281 kb on - strand, within glgBat 3570.288 kb on + strand, within glgBat 3570.288 kb on + strand, within glgBat 3570.325 kb on + strand, within glgBat 3570.325 kb on + strand, within glgBat 3570.345 kb on + strand, within glgBat 3570.345 kb on + strand, within glgBat 3570.403 kb on + strand, within glgBat 3570.406 kb on - strand, within glgBat 3570.406 kb on - strand, within glgBat 3570.406 kb on - strand, within glgBat 3570.419 kb on + strand, within glgBat 3570.444 kb on + strand, within glgBat 3570.459 kb on - strand, within glgBat 3570.484 kb on + strand, within glgBat 3570.491 kb on - strand, within glgBat 3570.491 kb on - strand, within glgBat 3570.492 kb on - strand, within glgBat 3570.506 kb on - strand, within glgBat 3570.517 kb on - strand, within glgBat 3570.645 kb on + strand, within glgBat 3570.665 kb on - strand, within glgBat 3570.675 kb on - strand, within glgBat 3570.679 kb on - strand, within glgBat 3570.679 kb on - strand, within glgBat 3570.705 kb on + strand, within glgBat 3570.733 kb on + strand, within glgBat 3570.832 kb on + strand, within glgBat 3570.849 kb on - strand, within glgBat 3570.890 kb on + strand, within glgBat 3570.913 kb on - strand, within glgBat 3570.941 kb on + strand, within glgBat 3570.968 kb on - strand, within glgBat 3571.005 kb on - strand, within glgBat 3571.005 kb on - strand, within glgBat 3571.036 kb on + strand, within glgBat 3571.125 kb on - strand, within glgBat 3571.125 kb on - strand, within glgBat 3571.179 kb on - strand, within glgBat 3571.179 kb on - strand, within glgBat 3571.189 kb on + strand, within glgBat 3571.227 kb on + strand, within glgBat 3571.227 kb on + strand, within glgBat 3571.269 kb on + strand, within glgBat 3571.322 kb on - strandat 3571.322 kb on - strandat 3571.384 kb on + strandat 3571.388 kb on - strandat 3571.398 kb on - strandat 3571.510 kb on + strandat 3571.510 kb on + strandat 3571.546 kb on + strandat 3571.554 kb on - strandat 3571.599 kb on - strandat 3571.606 kb on + strandat 3571.614 kb on - strandat 3571.624 kb on - strandat 3571.634 kb on - strandat 3571.659 kb on + strandat 3571.688 kb on - strandat 3571.702 kb on - strandat 3571.764 kb on - strandat 3571.771 kb on - strandat 3571.771 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63 no sulfur + 5 mM L-cys
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3,568,499 - glgX b3431 0.57 +0.2
3,568,590 - glgX b3431 0.62 -0.5
3,568,602 - glgX b3431 0.62 -0.6
3,568,641 + glgX b3431 0.64 +0.2
3,568,649 - glgX b3431 0.65 +1.4
3,568,649 - glgX b3431 0.65 -3.8
3,568,649 - glgX b3431 0.65 +0.2
3,568,682 + glgX b3431 0.67 -0.3
3,568,695 + glgX b3431 0.67 -2.0
3,568,695 + glgX b3431 0.67 -0.6
3,568,695 + glgX b3431 0.67 -0.7
3,568,701 + glgX b3431 0.67 -0.2
3,568,708 - glgX b3431 0.68 -0.3
3,568,708 - glgX b3431 0.68 +0.4
3,568,726 + glgX b3431 0.69 -0.4
3,568,842 - glgX b3431 0.75 -0.2
3,568,886 + glgX b3431 0.77 +0.5
3,568,886 + glgX b3431 0.77 +0.6
3,568,930 + glgX b3431 0.79 +0.4
3,568,932 + glgX b3431 0.79 +1.8
3,568,935 + glgX b3431 0.79 +0.6
3,568,946 - glgX b3431 0.80 +0.0
3,568,984 + glgX b3431 0.82 +0.1
3,568,984 + glgX b3431 0.82 +0.8
3,569,026 + glgX b3431 0.84 -0.7
3,569,026 + glgX b3431 0.84 +0.0
3,569,034 - glgX b3431 0.84 +1.0
3,569,114 + glgX b3431 0.88 +1.0
3,569,114 + glgX b3431 0.88 +0.4
3,569,151 - +0.3
3,569,151 - +0.8
3,569,159 + +0.2
3,569,159 + -0.1
3,569,192 - -0.2
3,569,192 - +0.1
3,569,240 + -0.2
3,569,240 + -0.3
3,569,248 - +0.3
3,569,248 - +0.7
3,569,248 - -0.6
3,569,277 + +0.2
3,569,288 + -0.2
3,569,328 + +0.6
3,569,357 - +0.4
3,569,357 - -0.6
3,569,359 + +0.1
3,569,359 + -0.9
3,569,366 - -0.3
3,569,389 + +0.3
3,569,429 + +0.2
3,569,429 + -0.9
3,569,432 + -0.5
3,569,432 + -0.1
3,569,437 - -0.5
3,569,437 - +0.7
3,569,437 - -0.4
3,569,437 - +0.1
3,569,442 - +0.9
3,569,442 - +0.6
3,569,452 + -0.9
3,569,460 - -0.1
3,569,460 - +1.7
3,569,460 - +0.9
3,569,516 + +0.3
3,569,523 - -2.6
3,569,523 - -0.3
3,569,541 + +0.6
3,569,541 + +0.3
3,569,556 + -1.5
3,569,556 + -0.5
3,569,635 - glgB b3432 0.14 -0.7
3,569,667 - glgB b3432 0.15 +0.5
3,569,702 - glgB b3432 0.17 +0.3
3,569,723 + glgB b3432 0.18 +0.6
3,569,779 - glgB b3432 0.20 -0.1
3,569,813 + glgB b3432 0.22 +0.2
3,569,828 - glgB b3432 0.22 +0.4
3,569,896 - glgB b3432 0.25 +0.4
3,569,903 + glgB b3432 0.26 -0.6
3,569,960 - glgB b3432 0.28 +0.2
3,569,960 - glgB b3432 0.28 -0.9
3,569,984 + glgB b3432 0.29 -0.4
3,569,984 + glgB b3432 0.29 +0.7
3,569,984 + glgB b3432 0.29 +0.3
3,570,002 + glgB b3432 0.30 -0.6
3,570,002 + glgB b3432 0.30 +0.1
3,570,007 + glgB b3432 0.31 -0.2
3,570,011 + glgB b3432 0.31 -0.1
3,570,014 - glgB b3432 0.31 -0.1
3,570,014 - glgB b3432 0.31 +1.1
3,570,015 - glgB b3432 0.31 +0.1
3,570,015 - glgB b3432 0.31 +0.3
3,570,061 - glgB b3432 0.33 -1.3
3,570,061 - glgB b3432 0.33 +0.1
3,570,062 + glgB b3432 0.33 +0.4
3,570,097 + glgB b3432 0.35 -0.1
3,570,105 - glgB b3432 0.35 +0.5
3,570,115 - glgB b3432 0.35 -0.6
3,570,116 - glgB b3432 0.36 +0.3
3,570,231 - glgB b3432 0.41 +0.3
3,570,281 - glgB b3432 0.43 -0.7
3,570,288 + glgB b3432 0.43 +0.4
3,570,288 + glgB b3432 0.43 +0.9
3,570,325 + glgB b3432 0.45 +0.3
3,570,325 + glgB b3432 0.45 +0.5
3,570,345 + glgB b3432 0.46 +0.2
3,570,345 + glgB b3432 0.46 -0.5
3,570,403 + glgB b3432 0.49 +1.2
3,570,406 - glgB b3432 0.49 -0.3
3,570,406 - glgB b3432 0.49 +0.2
3,570,406 - glgB b3432 0.49 +0.1
3,570,419 + glgB b3432 0.49 +0.7
3,570,444 + glgB b3432 0.51 +0.0
3,570,459 - glgB b3432 0.51 -0.1
3,570,484 + glgB b3432 0.52 +1.0
3,570,491 - glgB b3432 0.53 +0.1
3,570,491 - glgB b3432 0.53 +0.6
3,570,492 - glgB b3432 0.53 +0.2
3,570,506 - glgB b3432 0.53 +0.5
3,570,517 - glgB b3432 0.54 +0.1
3,570,645 + glgB b3432 0.60 +0.1
3,570,665 - glgB b3432 0.61 +0.5
3,570,675 - glgB b3432 0.61 +0.2
3,570,679 - glgB b3432 0.61 +0.2
3,570,679 - glgB b3432 0.61 -0.1
3,570,705 + glgB b3432 0.62 +0.6
3,570,733 + glgB b3432 0.64 -0.1
3,570,832 + glgB b3432 0.68 -0.1
3,570,849 - glgB b3432 0.69 -0.0
3,570,890 + glgB b3432 0.71 -0.1
3,570,913 - glgB b3432 0.72 +0.1
3,570,941 + glgB b3432 0.73 +0.2
3,570,968 - glgB b3432 0.74 -0.8
3,571,005 - glgB b3432 0.76 +0.8
3,571,005 - glgB b3432 0.76 -0.3
3,571,036 + glgB b3432 0.78 +0.3
3,571,125 - glgB b3432 0.82 +0.7
3,571,125 - glgB b3432 0.82 -0.1
3,571,179 - glgB b3432 0.84 +1.3
3,571,179 - glgB b3432 0.84 +0.2
3,571,189 + glgB b3432 0.85 +0.7
3,571,227 + glgB b3432 0.86 -1.5
3,571,227 + glgB b3432 0.86 +0.1
3,571,269 + glgB b3432 0.88 +0.3
3,571,322 - -0.1
3,571,322 - +0.9
3,571,384 + +0.3
3,571,388 - -0.0
3,571,398 - -0.7
3,571,510 + +1.0
3,571,510 + -0.2
3,571,546 + +0.1
3,571,554 - +0.2
3,571,599 - -0.2
3,571,606 + -0.3
3,571,614 - -0.2
3,571,624 - +0.3
3,571,634 - +0.1
3,571,659 + +0.0
3,571,688 - +0.0
3,571,702 - -0.6
3,571,764 - -1.5
3,571,771 - +0.6
3,571,771 - +1.3

Or see this region's nucleotide sequence