Strain Fitness in Escherichia coli BW25113 around b4177

Experiment: M63 no sulfur + 5 mM L-cys

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M63 no sulfur + 5 mM L-cys
4,401,756	-	hflC	b4175	0.43	-0.1
4,401,764	-	hflC	b4175	0.44	-0.4
4,401,764	-	hflC	b4175	0.44	-0.4
4,401,792	-	hflC	b4175	0.47	-0.7
4,401,792	-	hflC	b4175	0.47	-1.4
4,401,910	+	hflC	b4175	0.58	-0.6
4,401,912	+	hflC	b4175	0.59	+0.3
4,401,912	+	hflC	b4175	0.59	+0.0
4,401,912	+	hflC	b4175	0.59	-0.4
4,401,912	+	hflC	b4175	0.59	-0.0
4,401,920	-	hflC	b4175	0.59	-1.0
4,401,920	-	hflC	b4175	0.59	-0.6
4,401,920	-	hflC	b4175	0.59	+0.9
4,401,920	-	hflC	b4175	0.59	+0.9
4,401,927	+	hflC	b4175	0.60	+0.0
4,401,958	+	hflC	b4175	0.63	-0.6
4,401,986	-	hflC	b4175	0.66	+0.4
4,401,986	-	hflC	b4175	0.66	-0.3
4,401,997	-	hflC	b4175	0.67	-0.0
4,401,997	-	hflC	b4175	0.67	-0.5
4,402,038	+	hflC	b4175	0.71	+0.2
4,402,038	+	hflC	b4175	0.71	+0.5
4,402,126	-	hflC	b4175	0.80	-0.9
4,402,126	-	hflC	b4175	0.80	-0.5
4,402,138	-	hflC	b4175	0.81	+0.5
4,402,166	-	hflC	b4175	0.84	+0.5
4,402,191	-	hflC	b4175	0.86	-0.1
4,402,220	-	hflC	b4175	0.89	-0.6
4,402,220	-	hflC	b4175	0.89	-1.3
4,402,253	-				-0.6
4,402,253	-				+0.1
4,402,258	+				-0.3
4,402,263	-				-0.5
4,402,263	-				-0.2
4,402,263	-				-0.2
4,402,263	-				-0.5
4,402,326	+				+1.1
4,402,336	+				-0.8
4,402,336	+				+0.2
4,402,341	+				-0.3
4,402,373	+				-0.0
4,402,441	+	yjeT	b4176	0.16	-0.3
4,402,449	-	yjeT	b4176	0.20	-0.1
4,402,484	-	yjeT	b4176	0.38	+0.4
4,402,497	-	yjeT	b4176	0.44	+0.5
4,402,930	-	purA	b4177	0.17	-3.4
4,403,242	-	purA	b4177	0.41	-2.2
4,403,472	-	purA	b4177	0.59	-3.0
4,403,566	-	purA	b4177	0.66	-2.9
4,403,574	-	purA	b4177	0.67	-3.4
4,403,855	-	purA	b4177	0.88	-3.3
4,403,965	+				-0.7
4,403,967	-				-4.4
4,404,050	+				-0.1
4,404,050	+				-0.1
4,404,050	+				-0.3
4,404,050	+				-0.0
4,404,070	-				-0.1
4,404,095	-				+0.0
4,404,095	-				-0.4
4,404,097	+				-0.5
4,404,097	+				+0.1
4,404,169	-				-0.5
4,404,254	+				-0.4
4,404,311	+	yjeB	b4178	0.23	+0.4
4,404,311	+	yjeB	b4178	0.23	-0.7
4,404,312	+	yjeB	b4178	0.23	-0.5
4,404,312	+	yjeB	b4178	0.23	-0.7
4,404,364	-	yjeB	b4178	0.35	+0.7
4,404,364	-	yjeB	b4178	0.35	-0.3
4,404,379	-	yjeB	b4178	0.39	-1.4
4,404,379	-	yjeB	b4178	0.39	-1.6
4,404,490	-	yjeB	b4178	0.65	-0.6
4,404,536	+	yjeB	b4178	0.76	-0.2
4,404,536	+	yjeB	b4178	0.76	-0.3
4,404,536	+	yjeB	b4178	0.76	-0.4
4,404,536	+	yjeB	b4178	0.76	-0.7
4,404,536	+	yjeB	b4178	0.76	-0.2
4,404,544	-	yjeB	b4178	0.78	-0.4
4,404,544	-	yjeB	b4178	0.78	-0.0
4,404,567	+	yjeB	b4178	0.83	+0.6
4,404,583	-	yjeB	b4178	0.87	-1.0
4,404,600	-				+0.3
4,404,620	-				-0.3
4,404,695	+				-2.1
4,404,695	+				-0.9
4,404,806	-				-0.1
4,404,819	-				-0.2
4,404,873	-				-0.6
4,404,898	+				-1.4
4,404,898	+				-0.2
4,404,898	+				-1.5
4,404,928	+	vacB	b4179	0.10	+0.8
4,404,928	+	vacB	b4179	0.10	+0.6
4,404,952	+	vacB	b4179	0.11	-1.2
4,404,966	+	vacB	b4179	0.12	-1.0
4,404,966	+	vacB	b4179	0.12	-1.9