Experiment: M63 no sulfur + 5 mM L-cys
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptr and recC are separated by 175 nucleotides recC and ppdC are separated by 12 nucleotides ppdC and ygdB overlap by 16 nucleotides ygdB and ppdB overlap by 4 nucleotides
b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906
ptr
b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450
recC
b2823: ppdC - hypothetical protein (NCBI), at 2,960,463 to 2,960,786
ppdC
b2824: ygdB - orf, hypothetical protein (VIMSS), at 2,960,771 to 2,961,178
ygdB
b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738
ppdB
Position (kb)
2957
2958
2959
2960
2961 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2956.082 kb on - strand, within ptr at 2956.082 kb on - strand, within ptr at 2956.353 kb on - strand, within ptr at 2956.360 kb on + strand, within ptr at 2956.362 kb on + strand, within ptr at 2956.370 kb on - strand, within ptr at 2956.419 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.598 kb on - strand, within ptr at 2956.607 kb on - strand, within ptr at 2956.653 kb on + strand at 2956.658 kb on - strand at 2956.658 kb on - strand at 2956.663 kb on + strand at 2956.772 kb on - strand at 2956.772 kb on - strand at 2956.844 kb on + strand at 2956.882 kb on - strand at 2957.218 kb on - strand at 2957.288 kb on + strand at 2957.823 kb on + strand, within recC at 2958.415 kb on - strand, within recC at 2958.570 kb on - strand, within recC at 2958.570 kb on - strand, within recC at 2958.885 kb on - strand, within recC at 2959.105 kb on + strand, within recC at 2959.345 kb on + strand, within recC at 2959.585 kb on + strand, within recC at 2959.640 kb on + strand, within recC at 2959.672 kb on + strand, within recC at 2960.337 kb on - strand at 2960.370 kb on + strand at 2960.441 kb on + strand at 2960.489 kb on + strand at 2960.523 kb on - strand, within ppdC at 2960.523 kb on - strand, within ppdC at 2960.535 kb on + strand, within ppdC at 2960.661 kb on + strand, within ppdC at 2960.726 kb on - strand, within ppdC at 2960.726 kb on - strand, within ppdC at 2960.736 kb on + strand, within ppdC at 2960.759 kb on - strand at 2960.759 kb on - strand at 2960.974 kb on - strand, within ygdB at 2961.100 kb on + strand, within ygdB at 2961.100 kb on + strand, within ygdB at 2961.102 kb on - strand, within ygdB at 2961.141 kb on + strand at 2961.173 kb on + strand at 2961.343 kb on - strand, within ppdB
Per-strain Table
Position Strand Gene LocusTag Fraction M63 no sulfur + 5 mM L-cys remove 2,956,082 - ptr b2821 0.71 +0.3 2,956,082 - ptr b2821 0.71 +0.2 2,956,353 - ptr b2821 0.81 +0.1 2,956,360 + ptr b2821 0.81 +0.1 2,956,362 + ptr b2821 0.81 +0.6 2,956,370 - ptr b2821 0.81 -0.1 2,956,419 - ptr b2821 0.83 -1.5 2,956,478 - ptr b2821 0.85 +0.5 2,956,478 - ptr b2821 0.85 +0.3 2,956,485 + ptr b2821 0.85 +0.2 2,956,485 + ptr b2821 0.85 +0.1 2,956,598 - ptr b2821 0.89 +0.3 2,956,607 - ptr b2821 0.90 -0.2 2,956,653 + -0.0 2,956,658 - -0.9 2,956,658 - +0.4 2,956,663 + +0.1 2,956,772 - +0.2 2,956,772 - -0.3 2,956,844 + +0.3 2,956,882 - -0.1 2,957,218 - -0.5 2,957,288 + -1.8 2,957,823 + recC b2822 0.22 -2.1 2,958,415 - recC b2822 0.40 -0.7 2,958,570 - recC b2822 0.44 -0.9 2,958,570 - recC b2822 0.44 -2.2 2,958,885 - recC b2822 0.54 -2.1 2,959,105 + recC b2822 0.60 -2.3 2,959,345 + recC b2822 0.67 -2.6 2,959,585 + recC b2822 0.74 -0.3 2,959,640 + recC b2822 0.76 -3.2 2,959,672 + recC b2822 0.77 +0.1 2,960,337 - -0.7 2,960,370 + -2.5 2,960,441 + +1.9 2,960,489 + +0.0 2,960,523 - ppdC b2823 0.19 +0.3 2,960,523 - ppdC b2823 0.19 -0.1 2,960,535 + ppdC b2823 0.22 +0.3 2,960,661 + ppdC b2823 0.61 -0.2 2,960,726 - ppdC b2823 0.81 +1.0 2,960,726 - ppdC b2823 0.81 +0.9 2,960,736 + ppdC b2823 0.84 +0.0 2,960,759 - +1.3 2,960,759 - +0.8 2,960,974 - ygdB b2824 0.50 -0.0 2,961,100 + ygdB b2824 0.81 -0.2 2,961,100 + ygdB b2824 0.81 -0.3 2,961,102 - ygdB b2824 0.81 -0.9 2,961,141 + -0.4 2,961,173 + -0.2 2,961,343 - ppdB b2825 0.30 -0.3
Or see this region's nucleotide sequence