Experiment: M63 no sulfur + 5 mM L-cys
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcF and yfcG are separated by 135 nucleotides yfcG and folX are separated by 56 nucleotides folX and yfcH are separated by 20 nucleotides yfcH and yfcI are separated by 47 nucleotides
b2301: yfcF - predicted enzyme (NCBI), at 2,417,863 to 2,418,507
yfcF
b2302: yfcG - predicted glutathione S-transferase (NCBI), at 2,418,643 to 2,419,290
yfcG
b2303: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI), at 2,419,347 to 2,419,709
folX
b2304: yfcH - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,419,730 to 2,420,623
yfcH
b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561
yfcI
Position (kb)
2419
2420 Strain fitness (log2 ratio)
-1
0
1 at 2418.445 kb on - strand at 2418.572 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.714 kb on + strand, within yfcG at 2418.736 kb on + strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.744 kb on - strand, within yfcG at 2418.876 kb on - strand, within yfcG at 2418.934 kb on - strand, within yfcG at 2418.939 kb on - strand, within yfcG at 2418.970 kb on - strand, within yfcG at 2419.017 kb on - strand, within yfcG at 2419.052 kb on - strand, within yfcG at 2419.064 kb on + strand, within yfcG at 2419.077 kb on - strand, within yfcG at 2419.077 kb on - strand, within yfcG at 2419.108 kb on - strand, within yfcG at 2419.109 kb on - strand, within yfcG at 2419.186 kb on + strand, within yfcG at 2419.188 kb on + strand, within yfcG at 2419.213 kb on + strand, within yfcG at 2419.213 kb on + strand, within yfcG at 2419.222 kb on + strand, within yfcG at 2419.223 kb on - strand, within yfcG at 2419.241 kb on + strand at 2419.251 kb on + strand at 2419.267 kb on + strand at 2419.349 kb on + strand at 2419.398 kb on - strand, within folX at 2419.398 kb on - strand, within folX at 2419.452 kb on + strand, within folX at 2419.452 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on + strand, within folX at 2419.458 kb on - strand, within folX at 2419.458 kb on - strand, within folX at 2419.460 kb on - strand, within folX at 2419.460 kb on - strand, within folX at 2419.466 kb on - strand, within folX at 2419.466 kb on - strand, within folX at 2419.468 kb on - strand, within folX at 2419.468 kb on - strand, within folX at 2419.471 kb on + strand, within folX at 2419.471 kb on + strand, within folX at 2419.478 kb on + strand, within folX at 2419.494 kb on + strand, within folX at 2419.593 kb on + strand, within folX at 2419.603 kb on - strand, within folX at 2419.614 kb on - strand, within folX at 2419.619 kb on + strand, within folX at 2419.624 kb on - strand, within folX at 2419.630 kb on + strand, within folX at 2419.647 kb on + strand, within folX at 2419.647 kb on + strand, within folX at 2419.650 kb on + strand, within folX at 2419.653 kb on - strand, within folX at 2419.683 kb on + strand at 2419.689 kb on + strand at 2419.689 kb on + strand at 2419.697 kb on - strand at 2419.720 kb on - strand at 2419.720 kb on - strand at 2419.725 kb on - strand at 2419.726 kb on + strand at 2419.728 kb on + strand at 2419.736 kb on - strand at 2419.736 kb on - strand at 2419.809 kb on + strand at 2419.816 kb on + strand at 2419.816 kb on + strand at 2419.821 kb on + strand, within yfcH at 2419.821 kb on + strand, within yfcH at 2419.865 kb on + strand, within yfcH at 2419.874 kb on - strand, within yfcH at 2420.059 kb on - strand, within yfcH at 2420.077 kb on - strand, within yfcH at 2420.077 kb on - strand, within yfcH at 2420.089 kb on - strand, within yfcH at 2420.225 kb on - strand, within yfcH at 2420.225 kb on - strand, within yfcH at 2420.228 kb on - strand, within yfcH at 2420.228 kb on - strand, within yfcH at 2420.267 kb on + strand, within yfcH at 2420.267 kb on + strand, within yfcH at 2420.303 kb on + strand, within yfcH at 2420.346 kb on + strand, within yfcH at 2420.373 kb on - strand, within yfcH at 2420.373 kb on - strand, within yfcH at 2420.404 kb on - strand, within yfcH at 2420.404 kb on - strand, within yfcH at 2420.450 kb on - strand, within yfcH at 2420.450 kb on - strand, within yfcH at 2420.487 kb on + strand, within yfcH at 2420.487 kb on + strand, within yfcH at 2420.495 kb on - strand, within yfcH at 2420.521 kb on + strand, within yfcH at 2420.526 kb on + strand, within yfcH at 2420.632 kb on + strand at 2420.654 kb on + strand at 2420.677 kb on - strand at 2420.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M63 no sulfur + 5 mM L-cys remove 2,418,445 - -0.3 2,418,572 - -0.1 2,418,607 - +0.3 2,418,607 - +0.3 2,418,607 - -0.6 2,418,607 - +0.3 2,418,714 + yfcG b2302 0.11 -0.2 2,418,736 + yfcG b2302 0.14 +0.6 2,418,739 - yfcG b2302 0.15 +0.1 2,418,739 - yfcG b2302 0.15 -0.1 2,418,744 - yfcG b2302 0.16 -0.1 2,418,876 - yfcG b2302 0.36 +0.3 2,418,934 - yfcG b2302 0.45 +0.4 2,418,939 - yfcG b2302 0.46 -0.3 2,418,970 - yfcG b2302 0.50 -0.0 2,419,017 - yfcG b2302 0.58 -0.2 2,419,052 - yfcG b2302 0.63 -0.1 2,419,064 + yfcG b2302 0.65 -0.2 2,419,077 - yfcG b2302 0.67 -0.3 2,419,077 - yfcG b2302 0.67 -0.3 2,419,108 - yfcG b2302 0.72 +0.2 2,419,109 - yfcG b2302 0.72 +0.3 2,419,186 + yfcG b2302 0.84 +0.1 2,419,188 + yfcG b2302 0.84 -0.0 2,419,213 + yfcG b2302 0.88 -0.6 2,419,213 + yfcG b2302 0.88 -1.1 2,419,222 + yfcG b2302 0.89 +0.4 2,419,223 - yfcG b2302 0.90 +0.1 2,419,241 + -0.4 2,419,251 + -0.1 2,419,267 + -0.2 2,419,349 + +0.1 2,419,398 - folX b2303 0.14 +0.2 2,419,398 - folX b2303 0.14 +0.5 2,419,452 + folX b2303 0.29 -0.0 2,419,452 + folX b2303 0.29 -0.6 2,419,458 + folX b2303 0.31 +0.1 2,419,458 + folX b2303 0.31 +0.4 2,419,458 + folX b2303 0.31 +0.5 2,419,458 + folX b2303 0.31 +0.0 2,419,458 - folX b2303 0.31 +0.1 2,419,458 - folX b2303 0.31 +0.1 2,419,460 - folX b2303 0.31 +0.1 2,419,460 - folX b2303 0.31 +0.3 2,419,466 - folX b2303 0.33 -0.3 2,419,466 - folX b2303 0.33 -0.2 2,419,468 - folX b2303 0.33 -0.7 2,419,468 - folX b2303 0.33 +0.4 2,419,471 + folX b2303 0.34 +0.4 2,419,471 + folX b2303 0.34 +0.7 2,419,478 + folX b2303 0.36 -0.1 2,419,494 + folX b2303 0.40 -0.1 2,419,593 + folX b2303 0.68 +1.3 2,419,603 - folX b2303 0.71 -0.2 2,419,614 - folX b2303 0.74 +0.5 2,419,619 + folX b2303 0.75 +0.5 2,419,624 - folX b2303 0.76 +0.4 2,419,630 + folX b2303 0.78 -0.6 2,419,647 + folX b2303 0.83 +0.0 2,419,647 + folX b2303 0.83 +0.2 2,419,650 + folX b2303 0.83 -1.4 2,419,653 - folX b2303 0.84 -0.2 2,419,683 + +0.2 2,419,689 + +0.2 2,419,689 + +0.1 2,419,697 - +0.2 2,419,720 - +0.1 2,419,720 - +0.3 2,419,725 - +0.2 2,419,726 + -0.3 2,419,728 + -0.2 2,419,736 - +0.7 2,419,736 - +0.4 2,419,809 + -0.3 2,419,816 + +0.2 2,419,816 + +0.1 2,419,821 + yfcH b2304 0.10 +0.1 2,419,821 + yfcH b2304 0.10 +0.6 2,419,865 + yfcH b2304 0.15 +0.9 2,419,874 - yfcH b2304 0.16 +0.9 2,420,059 - yfcH b2304 0.37 -0.7 2,420,077 - yfcH b2304 0.39 -0.4 2,420,077 - yfcH b2304 0.39 -0.2 2,420,089 - yfcH b2304 0.40 +0.7 2,420,225 - yfcH b2304 0.55 -0.6 2,420,225 - yfcH b2304 0.55 +0.8 2,420,228 - yfcH b2304 0.56 +0.5 2,420,228 - yfcH b2304 0.56 +0.0 2,420,267 + yfcH b2304 0.60 -0.6 2,420,267 + yfcH b2304 0.60 -0.1 2,420,303 + yfcH b2304 0.64 +0.3 2,420,346 + yfcH b2304 0.69 -0.1 2,420,373 - yfcH b2304 0.72 +0.2 2,420,373 - yfcH b2304 0.72 -0.5 2,420,404 - yfcH b2304 0.75 -0.2 2,420,404 - yfcH b2304 0.75 -0.1 2,420,450 - yfcH b2304 0.81 +0.4 2,420,450 - yfcH b2304 0.81 +0.1 2,420,487 + yfcH b2304 0.85 +0.2 2,420,487 + yfcH b2304 0.85 -0.3 2,420,495 - yfcH b2304 0.86 +0.1 2,420,521 + yfcH b2304 0.88 +0.4 2,420,526 + yfcH b2304 0.89 -0.1 2,420,632 + -0.3 2,420,654 + -0.5 2,420,677 - -0.2 2,420,680 - +1.0
Or see this region's nucleotide sequence