Experiment: R2A with Ceftriaxone hemi(heptahydrate) 0.0005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1757 and Atu1758 are separated by 256 nucleotides Atu1758 and Atu1759 are separated by 65 nucleotides Atu1759 and Atu1760 are separated by 93 nucleotides Atu1760 and Atu8164 are separated by 269 nucleotides
Atu1757: Atu1757 - hypothetical protein, at 1,742,384 to 1,742,992
Atu1757
Atu1758: Atu1758 - hypothetical protein, at 1,743,249 to 1,743,683
Atu1758
Atu1759: Atu1759 - L-serine dehydratase, at 1,743,749 to 1,745,152
Atu1759
Atu1760: Atu1760 - hypothetical protein, at 1,745,246 to 1,745,536
Atu1760
Atu8164: Atu8164 - hypothetical protein, at 1,745,806 to 1,746,156
Atu8164
Position (kb)
1743
1744
1745
1746 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1742.779 kb on - strand, within Atu1757 at 1742.809 kb on - strand, within Atu1757 at 1742.873 kb on + strand, within Atu1757 at 1742.873 kb on + strand, within Atu1757 at 1743.003 kb on + strand at 1743.004 kb on + strand at 1743.017 kb on - strand at 1743.079 kb on + strand at 1743.095 kb on - strand at 1743.100 kb on + strand at 1743.108 kb on - strand at 1743.113 kb on - strand at 1743.118 kb on - strand at 1743.123 kb on + strand at 1743.166 kb on + strand at 1743.169 kb on - strand at 1743.169 kb on - strand at 1743.272 kb on + strand at 1743.399 kb on - strand, within Atu1758 at 1743.428 kb on + strand, within Atu1758 at 1743.429 kb on + strand, within Atu1758 at 1743.436 kb on - strand, within Atu1758 at 1743.505 kb on - strand, within Atu1758 at 1743.635 kb on + strand, within Atu1758 at 1743.643 kb on - strand at 1743.747 kb on + strand at 1743.760 kb on - strand at 1743.853 kb on - strand at 1743.856 kb on + strand at 1743.884 kb on - strand at 1743.936 kb on + strand, within Atu1759 at 1743.943 kb on + strand, within Atu1759 at 1743.974 kb on + strand, within Atu1759 at 1743.987 kb on - strand, within Atu1759 at 1744.100 kb on + strand, within Atu1759 at 1744.108 kb on - strand, within Atu1759 at 1744.108 kb on - strand, within Atu1759 at 1744.181 kb on + strand, within Atu1759 at 1744.241 kb on - strand, within Atu1759 at 1744.251 kb on + strand, within Atu1759 at 1744.259 kb on - strand, within Atu1759 at 1744.352 kb on - strand, within Atu1759 at 1744.411 kb on + strand, within Atu1759 at 1744.419 kb on - strand, within Atu1759 at 1744.419 kb on - strand, within Atu1759 at 1744.616 kb on - strand, within Atu1759 at 1744.641 kb on + strand, within Atu1759 at 1744.659 kb on - strand, within Atu1759 at 1744.674 kb on - strand, within Atu1759 at 1744.681 kb on + strand, within Atu1759 at 1744.827 kb on + strand, within Atu1759 at 1744.842 kb on + strand, within Atu1759 at 1745.006 kb on - strand, within Atu1759 at 1745.006 kb on - strand, within Atu1759 at 1745.108 kb on - strand at 1745.155 kb on + strand at 1745.197 kb on - strand at 1745.439 kb on + strand, within Atu1760 at 1745.669 kb on - strand at 1745.739 kb on - strand at 1745.760 kb on - strand at 1745.854 kb on - strand, within Atu8164 at 1745.879 kb on + strand, within Atu8164 at 1745.880 kb on + strand, within Atu8164 at 1745.880 kb on + strand, within Atu8164 at 1745.880 kb on + strand, within Atu8164 at 1745.887 kb on + strand, within Atu8164 at 1745.888 kb on - strand, within Atu8164 at 1745.895 kb on - strand, within Atu8164 at 1745.964 kb on - strand, within Atu8164 at 1745.984 kb on + strand, within Atu8164 at 1746.057 kb on + strand, within Atu8164 at 1746.066 kb on + strand, within Atu8164 at 1746.103 kb on + strand, within Atu8164 at 1746.103 kb on + strand, within Atu8164 at 1746.103 kb on + strand, within Atu8164 at 1746.111 kb on - strand, within Atu8164
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftriaxone hemi(heptahydrate) 0.0005 mM remove 1,742,779 - Atu1757 0.65 +1.2 1,742,809 - Atu1757 0.70 +0.5 1,742,873 + Atu1757 0.80 +0.7 1,742,873 + Atu1757 0.80 +0.5 1,743,003 + +0.4 1,743,004 + -0.4 1,743,017 - -0.2 1,743,079 + -1.2 1,743,095 - +0.0 1,743,100 + -1.0 1,743,108 - -0.1 1,743,113 - +0.8 1,743,118 - +0.2 1,743,123 + +0.0 1,743,166 + -0.9 1,743,169 - +0.3 1,743,169 - -0.1 1,743,272 + -0.5 1,743,399 - Atu1758 0.34 +0.0 1,743,428 + Atu1758 0.41 -0.9 1,743,429 + Atu1758 0.41 +0.2 1,743,436 - Atu1758 0.43 +0.0 1,743,505 - Atu1758 0.59 -0.2 1,743,635 + Atu1758 0.89 +0.0 1,743,643 - +0.1 1,743,747 + -0.1 1,743,760 - -0.3 1,743,853 - -1.2 1,743,856 + -0.1 1,743,884 - +0.1 1,743,936 + Atu1759 0.13 -1.3 1,743,943 + Atu1759 0.14 -0.8 1,743,974 + Atu1759 0.16 +0.1 1,743,987 - Atu1759 0.17 -1.3 1,744,100 + Atu1759 0.25 -0.7 1,744,108 - Atu1759 0.26 -0.3 1,744,108 - Atu1759 0.26 -0.1 1,744,181 + Atu1759 0.31 -1.1 1,744,241 - Atu1759 0.35 -0.7 1,744,251 + Atu1759 0.36 -1.2 1,744,259 - Atu1759 0.36 -0.7 1,744,352 - Atu1759 0.43 -1.7 1,744,411 + Atu1759 0.47 -0.3 1,744,419 - Atu1759 0.48 -0.2 1,744,419 - Atu1759 0.48 -1.1 1,744,616 - Atu1759 0.62 -1.8 1,744,641 + Atu1759 0.64 +0.0 1,744,659 - Atu1759 0.65 -1.0 1,744,674 - Atu1759 0.66 +0.3 1,744,681 + Atu1759 0.66 -0.8 1,744,827 + Atu1759 0.77 -0.5 1,744,842 + Atu1759 0.78 -0.6 1,745,006 - Atu1759 0.90 +2.4 1,745,006 - Atu1759 0.90 -1.1 1,745,108 - -1.1 1,745,155 + -0.6 1,745,197 - -0.4 1,745,439 + Atu1760 0.66 -0.4 1,745,669 - +0.4 1,745,739 - -0.1 1,745,760 - +0.0 1,745,854 - Atu8164 0.14 -0.7 1,745,879 + Atu8164 0.21 +0.5 1,745,880 + Atu8164 0.21 -0.1 1,745,880 + Atu8164 0.21 -0.2 1,745,880 + Atu8164 0.21 -0.2 1,745,887 + Atu8164 0.23 -0.1 1,745,888 - Atu8164 0.23 +0.3 1,745,895 - Atu8164 0.25 +0.8 1,745,964 - Atu8164 0.45 -0.4 1,745,984 + Atu8164 0.51 +0.0 1,746,057 + Atu8164 0.72 +0.8 1,746,066 + Atu8164 0.74 -0.1 1,746,103 + Atu8164 0.85 -0.0 1,746,103 + Atu8164 0.85 +0.2 1,746,103 + Atu8164 0.85 +0.4 1,746,111 - Atu8164 0.87 +0.7
Or see this region's nucleotide sequence