Experiment: R2A with Novobiocin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4216 and Atu4217 are separated by 146 nucleotides Atu4217 and Atu4218 are separated by 97 nucleotides Atu4218 and Atu4219 overlap by 1 nucleotides
Atu4216: Atu4216 - 3-oxoacyl-ACP synthase, at 1,337,832 to 1,339,130
Atu4216
Atu4217: Atu4217 - TetR family transcriptional regulator, at 1,339,277 to 1,339,864
Atu4217
Atu4218: Atu4218 - multidrug efflux protein, at 1,339,962 to 1,341,071
Atu4218
Atu4219: Atu4219 - cation efflux system protein, at 1,341,071 to 1,344,181
Atu4219
Position (kb)
1339
1340
1341
1342 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1338.982 kb on - strand, within Atu4216 at 1339.043 kb on + strand at 1339.071 kb on + strand at 1339.071 kb on + strand at 1339.180 kb on + strand at 1339.180 kb on + strand at 1339.229 kb on + strand at 1339.229 kb on + strand at 1339.229 kb on + strand at 1339.273 kb on + strand at 1339.575 kb on + strand, within Atu4217 at 1339.618 kb on + strand, within Atu4217 at 1339.776 kb on - strand, within Atu4217 at 1339.947 kb on + strand at 1339.991 kb on + strand at 1340.077 kb on + strand, within Atu4218 at 1340.085 kb on + strand, within Atu4218 at 1340.093 kb on - strand, within Atu4218 at 1340.103 kb on - strand, within Atu4218 at 1340.120 kb on - strand, within Atu4218 at 1340.167 kb on - strand, within Atu4218 at 1340.184 kb on - strand, within Atu4218 at 1340.236 kb on - strand, within Atu4218 at 1340.237 kb on - strand, within Atu4218 at 1340.271 kb on - strand, within Atu4218 at 1340.288 kb on - strand, within Atu4218 at 1340.369 kb on - strand, within Atu4218 at 1340.378 kb on - strand, within Atu4218 at 1340.412 kb on - strand, within Atu4218 at 1340.428 kb on - strand, within Atu4218 at 1340.460 kb on - strand, within Atu4218 at 1340.503 kb on - strand, within Atu4218 at 1340.532 kb on - strand, within Atu4218 at 1340.552 kb on + strand, within Atu4218 at 1340.590 kb on - strand, within Atu4218 at 1340.604 kb on + strand, within Atu4218 at 1340.604 kb on + strand, within Atu4218 at 1340.684 kb on + strand, within Atu4218 at 1340.754 kb on + strand, within Atu4218 at 1340.760 kb on - strand, within Atu4218 at 1340.796 kb on + strand, within Atu4218 at 1340.804 kb on - strand, within Atu4218 at 1340.833 kb on + strand, within Atu4218 at 1340.856 kb on + strand, within Atu4218 at 1340.869 kb on - strand, within Atu4218 at 1340.880 kb on + strand, within Atu4218 at 1340.882 kb on + strand, within Atu4218 at 1340.882 kb on + strand, within Atu4218 at 1340.888 kb on - strand, within Atu4218 at 1340.900 kb on - strand, within Atu4218 at 1340.911 kb on - strand, within Atu4218 at 1340.918 kb on + strand, within Atu4218 at 1340.927 kb on + strand, within Atu4218 at 1340.939 kb on + strand, within Atu4218 at 1340.945 kb on - strand, within Atu4218 at 1340.951 kb on + strand, within Atu4218 at 1341.025 kb on - strand at 1341.075 kb on + strand at 1341.075 kb on + strand at 1341.083 kb on - strand at 1341.102 kb on - strand at 1341.209 kb on - strand at 1341.251 kb on + strand at 1341.259 kb on - strand at 1341.267 kb on - strand at 1341.298 kb on + strand at 1341.306 kb on - strand at 1341.446 kb on - strand, within Atu4219 at 1341.478 kb on + strand, within Atu4219 at 1341.508 kb on + strand, within Atu4219 at 1341.553 kb on - strand, within Atu4219 at 1341.568 kb on + strand, within Atu4219 at 1341.682 kb on + strand, within Atu4219 at 1341.755 kb on + strand, within Atu4219 at 1341.784 kb on - strand, within Atu4219 at 1341.793 kb on + strand, within Atu4219 at 1341.859 kb on + strand, within Atu4219 at 1341.954 kb on + strand, within Atu4219 at 1341.962 kb on - strand, within Atu4219 at 1341.962 kb on - strand, within Atu4219 at 1341.972 kb on - strand, within Atu4219 at 1341.985 kb on + strand, within Atu4219 at 1342.017 kb on + strand, within Atu4219
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Novobiocin 0.002 mM remove 1,338,982 - Atu4216 0.89 +0.3 1,339,043 + -0.3 1,339,071 + +0.3 1,339,071 + +0.1 1,339,180 + +0.4 1,339,180 + -0.3 1,339,229 + +0.0 1,339,229 + +1.0 1,339,229 + -0.1 1,339,273 + +0.9 1,339,575 + Atu4217 0.51 -2.2 1,339,618 + Atu4217 0.58 -1.5 1,339,776 - Atu4217 0.85 -3.0 1,339,947 + -0.2 1,339,991 + -0.2 1,340,077 + Atu4218 0.10 +0.1 1,340,085 + Atu4218 0.11 +0.2 1,340,093 - Atu4218 0.12 -0.6 1,340,103 - Atu4218 0.13 +0.7 1,340,120 - Atu4218 0.14 -0.1 1,340,167 - Atu4218 0.18 -0.0 1,340,184 - Atu4218 0.20 -0.9 1,340,236 - Atu4218 0.25 -1.9 1,340,237 - Atu4218 0.25 -0.2 1,340,271 - Atu4218 0.28 +1.1 1,340,288 - Atu4218 0.29 -0.1 1,340,369 - Atu4218 0.37 -0.0 1,340,378 - Atu4218 0.37 -0.1 1,340,412 - Atu4218 0.41 +0.7 1,340,428 - Atu4218 0.42 -0.4 1,340,460 - Atu4218 0.45 -0.1 1,340,503 - Atu4218 0.49 +0.3 1,340,532 - Atu4218 0.51 +0.2 1,340,552 + Atu4218 0.53 -0.4 1,340,590 - Atu4218 0.57 -0.1 1,340,604 + Atu4218 0.58 -0.5 1,340,604 + Atu4218 0.58 -0.1 1,340,684 + Atu4218 0.65 -0.5 1,340,754 + Atu4218 0.71 +0.6 1,340,760 - Atu4218 0.72 -0.1 1,340,796 + Atu4218 0.75 +0.1 1,340,804 - Atu4218 0.76 +0.1 1,340,833 + Atu4218 0.78 +0.2 1,340,856 + Atu4218 0.81 -0.4 1,340,869 - Atu4218 0.82 -0.1 1,340,880 + Atu4218 0.83 +1.2 1,340,882 + Atu4218 0.83 -0.2 1,340,882 + Atu4218 0.83 +0.0 1,340,888 - Atu4218 0.83 -0.3 1,340,900 - Atu4218 0.85 +0.1 1,340,911 - Atu4218 0.85 +0.2 1,340,918 + Atu4218 0.86 +0.2 1,340,927 + Atu4218 0.87 -0.1 1,340,939 + Atu4218 0.88 +0.1 1,340,945 - Atu4218 0.89 -0.9 1,340,951 + Atu4218 0.89 +0.2 1,341,025 - +0.1 1,341,075 + -0.1 1,341,075 + +0.0 1,341,083 - +0.1 1,341,102 - +0.1 1,341,209 - -0.7 1,341,251 + -0.3 1,341,259 - +0.3 1,341,267 - +0.2 1,341,298 + +0.5 1,341,306 - -0.1 1,341,446 - Atu4219 0.12 -0.9 1,341,478 + Atu4219 0.13 +0.2 1,341,508 + Atu4219 0.14 -0.8 1,341,553 - Atu4219 0.15 -0.0 1,341,568 + Atu4219 0.16 -0.1 1,341,682 + Atu4219 0.20 -0.2 1,341,755 + Atu4219 0.22 +2.0 1,341,784 - Atu4219 0.23 +0.0 1,341,793 + Atu4219 0.23 +0.8 1,341,859 + Atu4219 0.25 -0.0 1,341,954 + Atu4219 0.28 +0.3 1,341,962 - Atu4219 0.29 -0.8 1,341,962 - Atu4219 0.29 +0.6 1,341,972 - Atu4219 0.29 -0.7 1,341,985 + Atu4219 0.29 -0.6 1,342,017 + Atu4219 0.30 -0.4
Or see this region's nucleotide sequence