Strain Fitness in Agrobacterium fabrum C58 around Atu2084

Experiment: R2A with Novobiocin 0.002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu2083 and Atu2084 are separated by 15 nucleotidesAtu2084 and Atu2085 are separated by 234 nucleotides Atu2083: Atu2083 - DNA repair protein RecN, at 2,043,102 to 2,044,775 Atu2083 Atu2084: Atu2084 - hypothetical protein, at 2,044,791 to 2,045,657 Atu2084 Atu2085: Atu2085 - UDP-3-0-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase, at 2,045,892 to 2,046,848 Atu2085 Position (kb) 2044 2045 2046Strain fitness (log2 ratio) -3 -2 -1 0 1at 2043.957 kb on - strand, within Atu2083at 2044.238 kb on + strand, within Atu2083at 2044.546 kb on - strand, within Atu2083at 2044.712 kb on + strandat 2044.779 kb on - strandat 2044.785 kb on - strandat 2044.882 kb on - strand, within Atu2084

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Novobiocin 0.002 mM
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2,043,957 - Atu2083 0.51 -1.0
2,044,238 + Atu2083 0.68 +1.3
2,044,546 - Atu2083 0.86 -1.1
2,044,712 + +0.3
2,044,779 - -0.7
2,044,785 - -0.4
2,044,882 - Atu2084 0.10 -3.4

Or see this region's nucleotide sequence