Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4074 and Atu4075 are separated by 4 nucleotides Atu4075 and Atu4076 are separated by 5 nucleotides
Atu4074: Atu4074 - phosphoglucomutase, at 1,191,284 to 1,192,912
Atu4074
Atu4075: Atu4075 - glycogen synthase, at 1,192,917 to 1,194,359
Atu4075
Atu4076: Atu4076 - glucose-1-phosphate adenylyltransferase, at 1,194,365 to 1,195,627
Atu4076
Position (kb)
1192
1193
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1 at 1193.074 kb on - strand, within Atu4075 at 1193.206 kb on + strand, within Atu4075 at 1193.206 kb on + strand, within Atu4075 at 1193.214 kb on - strand, within Atu4075 at 1193.214 kb on - strand, within Atu4075 at 1193.421 kb on - strand, within Atu4075 at 1193.438 kb on + strand, within Atu4075 at 1193.647 kb on - strand, within Atu4075 at 1194.018 kb on - strand, within Atu4075 at 1194.046 kb on + strand, within Atu4075 at 1194.130 kb on - strand, within Atu4075 at 1194.143 kb on - strand, within Atu4075 at 1194.173 kb on + strand, within Atu4075 at 1194.214 kb on + strand, within Atu4075 at 1194.244 kb on - strand at 1194.321 kb on - strand at 1194.343 kb on + strand at 1194.510 kb on - strand, within Atu4076 at 1194.591 kb on + strand, within Atu4076 at 1194.599 kb on - strand, within Atu4076 at 1194.669 kb on - strand, within Atu4076 at 1194.713 kb on + strand, within Atu4076 at 1194.725 kb on + strand, within Atu4076 at 1194.733 kb on - strand, within Atu4076 at 1194.786 kb on + strand, within Atu4076 at 1194.888 kb on + strand, within Atu4076 at 1194.896 kb on - strand, within Atu4076 at 1194.896 kb on - strand, within Atu4076 at 1195.002 kb on + strand, within Atu4076 at 1195.119 kb on - strand, within Atu4076 at 1195.194 kb on - strand, within Atu4076 at 1195.262 kb on - strand, within Atu4076
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,193,074 - Atu4075 0.11 -2.6 1,193,206 + Atu4075 0.20 -0.0 1,193,206 + Atu4075 0.20 -1.3 1,193,214 - Atu4075 0.21 -1.0 1,193,214 - Atu4075 0.21 +0.3 1,193,421 - Atu4075 0.35 -0.5 1,193,438 + Atu4075 0.36 -0.7 1,193,647 - Atu4075 0.51 -0.8 1,194,018 - Atu4075 0.76 +0.3 1,194,046 + Atu4075 0.78 -0.4 1,194,130 - Atu4075 0.84 -1.3 1,194,143 - Atu4075 0.85 -0.9 1,194,173 + Atu4075 0.87 -0.5 1,194,214 + Atu4075 0.90 +1.5 1,194,244 - -0.4 1,194,321 - -0.3 1,194,343 + -0.7 1,194,510 - Atu4076 0.11 +0.0 1,194,591 + Atu4076 0.18 +0.2 1,194,599 - Atu4076 0.19 -0.2 1,194,669 - Atu4076 0.24 +0.1 1,194,713 + Atu4076 0.28 -0.2 1,194,725 + Atu4076 0.29 -0.4 1,194,733 - Atu4076 0.29 +0.4 1,194,786 + Atu4076 0.33 +0.3 1,194,888 + Atu4076 0.41 +0.2 1,194,896 - Atu4076 0.42 -0.0 1,194,896 - Atu4076 0.42 +0.5 1,195,002 + Atu4076 0.50 +0.2 1,195,119 - Atu4076 0.60 +0.5 1,195,194 - Atu4076 0.66 +0.4 1,195,262 - Atu4076 0.71 +0.8
Or see this region's nucleotide sequence