Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3591 and Atu8190 are separated by 17 nucleotides Atu8190 and Atu3593 are separated by 1 nucleotides Atu3593 and Atu3594 are separated by 32 nucleotides Atu3594 and Atu3595 are separated by 182 nucleotides
Atu3591: Atu3591 - short chain dehydrogenase, at 647,058 to 647,891
Atu3591
Atu8190: Atu8190 - hypothetical protein, at 647,909 to 648,106
Atu8190
Atu3593: Atu3593 - hypothetical protein, at 648,108 to 648,686
Atu3593
Atu3594: Atu3594 - hypothetical protein, at 648,719 to 649,492
Atu3594
Atu3595: Atu3595 - electron transfer flavoprotein subunit beta, at 649,675 to 650,421
Atu3595
Position (kb) 648
649 Strain fitness (log2 ratio) -1
0
1 at 647.147 kb on + strand, within Atu3591 at 647.203 kb on + strand, within Atu3591 at 647.295 kb on + strand, within Atu3591 at 647.397 kb on - strand, within Atu3591 at 647.434 kb on + strand, within Atu3591 at 647.443 kb on + strand, within Atu3591 at 647.471 kb on - strand, within Atu3591 at 647.495 kb on + strand, within Atu3591 at 647.553 kb on + strand, within Atu3591 at 647.553 kb on - strand, within Atu3591 at 647.553 kb on - strand, within Atu3591 at 647.566 kb on + strand, within Atu3591 at 647.590 kb on - strand, within Atu3591 at 647.630 kb on - strand, within Atu3591 at 647.639 kb on + strand, within Atu3591 at 647.647 kb on - strand, within Atu3591 at 647.748 kb on + strand, within Atu3591 at 647.767 kb on - strand, within Atu3591 at 647.769 kb on + strand, within Atu3591 at 647.772 kb on - strand, within Atu3591 at 647.795 kb on + strand, within Atu3591 at 647.803 kb on - strand, within Atu3591 at 647.831 kb on - strand at 647.869 kb on - strand at 647.869 kb on - strand at 647.902 kb on + strand at 647.904 kb on - strand at 647.927 kb on + strand at 647.930 kb on - strand, within Atu8190 at 647.935 kb on - strand, within Atu8190 at 647.935 kb on - strand, within Atu8190 at 647.943 kb on + strand, within Atu8190 at 647.978 kb on + strand, within Atu8190 at 647.988 kb on - strand, within Atu8190 at 648.105 kb on + strand at 648.214 kb on - strand, within Atu3593 at 648.254 kb on + strand, within Atu3593 at 648.262 kb on - strand, within Atu3593 at 648.335 kb on + strand, within Atu3593 at 648.384 kb on + strand, within Atu3593 at 648.451 kb on + strand, within Atu3593 at 648.489 kb on + strand, within Atu3593 at 648.537 kb on - strand, within Atu3593 at 648.543 kb on - strand, within Atu3593 at 648.687 kb on + strand at 648.690 kb on - strand at 648.704 kb on + strand at 648.712 kb on - strand at 648.728 kb on + strand at 648.800 kb on + strand, within Atu3594 at 648.808 kb on - strand, within Atu3594 at 648.813 kb on - strand, within Atu3594 at 648.839 kb on - strand, within Atu3594 at 648.844 kb on + strand, within Atu3594 at 648.844 kb on + strand, within Atu3594 at 648.846 kb on - strand, within Atu3594 at 648.852 kb on - strand, within Atu3594 at 648.878 kb on - strand, within Atu3594 at 648.911 kb on - strand, within Atu3594 at 648.944 kb on + strand, within Atu3594 at 649.166 kb on - strand, within Atu3594 at 649.173 kb on + strand, within Atu3594 at 649.213 kb on - strand, within Atu3594 at 649.221 kb on + strand, within Atu3594 at 649.239 kb on - strand, within Atu3594 at 649.250 kb on - strand, within Atu3594 at 649.268 kb on + strand, within Atu3594 at 649.337 kb on + strand, within Atu3594 at 649.382 kb on - strand, within Atu3594 at 649.399 kb on + strand, within Atu3594 at 649.409 kb on - strand, within Atu3594 at 649.416 kb on + strand at 649.480 kb on - strand at 649.491 kb on - strand at 649.491 kb on - strand at 649.573 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 647,147 + Atu3591 0.11 -0.0 647,203 + Atu3591 0.17 -0.0 647,295 + Atu3591 0.28 -1.1 647,397 - Atu3591 0.41 -1.5 647,434 + Atu3591 0.45 +0.7 647,443 + Atu3591 0.46 +0.1 647,471 - Atu3591 0.50 +1.0 647,495 + Atu3591 0.52 +0.1 647,553 + Atu3591 0.59 -0.4 647,553 - Atu3591 0.59 -0.1 647,553 - Atu3591 0.59 -0.4 647,566 + Atu3591 0.61 +1.3 647,590 - Atu3591 0.64 +0.1 647,630 - Atu3591 0.69 -0.1 647,639 + Atu3591 0.70 -0.4 647,647 - Atu3591 0.71 -0.2 647,748 + Atu3591 0.83 -0.0 647,767 - Atu3591 0.85 +0.2 647,769 + Atu3591 0.85 -0.7 647,772 - Atu3591 0.86 +0.7 647,795 + Atu3591 0.88 +0.5 647,803 - Atu3591 0.89 +0.9 647,831 - -0.9 647,869 - +0.5 647,869 - +0.3 647,902 + -0.1 647,904 - +0.2 647,927 + -0.8 647,930 - Atu8190 0.11 +0.4 647,935 - Atu8190 0.13 -0.9 647,935 - Atu8190 0.13 -1.6 647,943 + Atu8190 0.17 -1.4 647,978 + Atu8190 0.35 -1.5 647,988 - Atu8190 0.40 +0.6 648,105 + -0.5 648,214 - Atu3593 0.18 -1.2 648,254 + Atu3593 0.25 -1.3 648,262 - Atu3593 0.27 -0.3 648,335 + Atu3593 0.39 +0.3 648,384 + Atu3593 0.48 -1.2 648,451 + Atu3593 0.59 -0.3 648,489 + Atu3593 0.66 -0.8 648,537 - Atu3593 0.74 -0.2 648,543 - Atu3593 0.75 -0.8 648,687 + -0.4 648,690 - -0.4 648,704 + -1.0 648,712 - -0.3 648,728 + +1.1 648,800 + Atu3594 0.10 +0.3 648,808 - Atu3594 0.11 +1.7 648,813 - Atu3594 0.12 -0.2 648,839 - Atu3594 0.16 +0.4 648,844 + Atu3594 0.16 +1.7 648,844 + Atu3594 0.16 +0.2 648,846 - Atu3594 0.16 -0.0 648,852 - Atu3594 0.17 +0.6 648,878 - Atu3594 0.21 -0.0 648,911 - Atu3594 0.25 +0.2 648,944 + Atu3594 0.29 +0.5 649,166 - Atu3594 0.58 -0.4 649,173 + Atu3594 0.59 -0.1 649,213 - Atu3594 0.64 +0.1 649,221 + Atu3594 0.65 +0.0 649,239 - Atu3594 0.67 +0.0 649,250 - Atu3594 0.69 +0.2 649,268 + Atu3594 0.71 +0.6 649,337 + Atu3594 0.80 +0.3 649,382 - Atu3594 0.86 +0.3 649,399 + Atu3594 0.88 +0.6 649,409 - Atu3594 0.89 +0.1 649,416 + -1.0 649,480 - -0.3 649,491 - -0.2 649,491 - +0.9 649,573 + -1.1
Or see this region's nucleotide sequence