Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3581 and Atu3582 are separated by 4 nucleotides Atu3582 and Atu3583 are separated by 17 nucleotides Atu3583 and Atu8189 are separated by 34 nucleotides
Atu3581: Atu3581 - propionyl-coenzyme A carboxylase, alpha polypeptide, at 636,779 to 638,782
Atu3581
Atu3582: Atu3582 - hypothetical protein, at 638,787 to 639,206
Atu3582
Atu3583: Atu3583 - hypothetical protein, at 639,224 to 639,784
Atu3583
Atu8189: Atu8189 - hypothetical protein, at 639,819 to 640,196
Atu8189
Position (kb) 638
639
640 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 637.788 kb on + strand, within Atu3581 at 637.803 kb on - strand, within Atu3581 at 637.834 kb on + strand, within Atu3581 at 638.038 kb on + strand, within Atu3581 at 638.107 kb on - strand, within Atu3581 at 638.224 kb on + strand, within Atu3581 at 638.277 kb on + strand, within Atu3581 at 638.277 kb on + strand, within Atu3581 at 638.315 kb on - strand, within Atu3581 at 638.372 kb on - strand, within Atu3581 at 638.382 kb on - strand, within Atu3581 at 638.385 kb on - strand, within Atu3581 at 638.599 kb on + strand at 638.601 kb on + strand at 638.601 kb on + strand at 638.601 kb on + strand at 638.607 kb on - strand at 638.609 kb on - strand at 638.628 kb on - strand at 638.638 kb on - strand at 638.638 kb on - strand at 638.688 kb on - strand at 638.877 kb on + strand, within Atu3582 at 638.970 kb on + strand, within Atu3582 at 639.011 kb on + strand, within Atu3582 at 639.013 kb on + strand, within Atu3582 at 639.016 kb on + strand, within Atu3582 at 639.026 kb on + strand, within Atu3582 at 639.186 kb on + strand at 639.338 kb on + strand, within Atu3583 at 639.346 kb on - strand, within Atu3583 at 639.346 kb on - strand, within Atu3583 at 639.463 kb on + strand, within Atu3583 at 639.582 kb on + strand, within Atu3583 at 639.590 kb on - strand, within Atu3583 at 639.743 kb on + strand at 639.748 kb on + strand at 639.756 kb on - strand at 639.756 kb on - strand at 639.756 kb on - strand at 639.855 kb on + strand at 639.887 kb on - strand, within Atu8189 at 639.894 kb on - strand, within Atu8189 at 639.894 kb on - strand, within Atu8189 at 640.041 kb on + strand, within Atu8189 at 640.056 kb on + strand, within Atu8189 at 640.099 kb on - strand, within Atu8189 at 640.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 637,788 + Atu3581 0.50 +0.1 637,803 - Atu3581 0.51 -1.1 637,834 + Atu3581 0.53 +0.4 638,038 + Atu3581 0.63 -0.2 638,107 - Atu3581 0.66 +0.3 638,224 + Atu3581 0.72 +0.4 638,277 + Atu3581 0.75 -0.1 638,277 + Atu3581 0.75 -0.3 638,315 - Atu3581 0.77 -0.3 638,372 - Atu3581 0.79 +1.4 638,382 - Atu3581 0.80 +0.2 638,385 - Atu3581 0.80 +0.5 638,599 + +0.0 638,601 + -0.3 638,601 + +0.0 638,601 + +0.4 638,607 - +0.2 638,609 - +0.3 638,628 - -0.3 638,638 - +0.4 638,638 - -0.2 638,688 - -0.0 638,877 + Atu3582 0.21 +0.3 638,970 + Atu3582 0.44 +0.3 639,011 + Atu3582 0.53 -0.2 639,013 + Atu3582 0.54 +0.6 639,016 + Atu3582 0.55 -0.0 639,026 + Atu3582 0.57 +1.7 639,186 + +1.0 639,338 + Atu3583 0.20 -0.2 639,346 - Atu3583 0.22 +0.2 639,346 - Atu3583 0.22 -0.3 639,463 + Atu3583 0.43 -2.9 639,582 + Atu3583 0.64 +0.2 639,590 - Atu3583 0.65 +0.4 639,743 + +0.2 639,748 + +0.2 639,756 - -1.0 639,756 - +0.2 639,756 - +0.2 639,855 + +0.2 639,887 - Atu8189 0.18 -0.2 639,894 - Atu8189 0.20 +1.5 639,894 - Atu8189 0.20 +0.3 640,041 + Atu8189 0.59 +0.2 640,056 + Atu8189 0.63 +0.2 640,099 - Atu8189 0.74 +0.1 640,204 + +0.1
Or see this region's nucleotide sequence