Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3489 and Atu3490 overlap by 4 nucleotides Atu3490 and Atu3491 are separated by 3 nucleotides Atu3491 and Atu3492 overlap by 4 nucleotides
Atu3489: Atu3489 - ABC transporter permease, at 540,191 to 541,192
Atu3489
Atu3490: Atu3490 - ABC transporter permease, at 541,189 to 542,199
Atu3490
Atu3491: Atu3491 - hypothetical protein, at 542,203 to 542,526
Atu3491
Atu3492: Atu3492 - sugar kinase, at 542,523 to 543,905
Atu3492
Position (kb) 541
542
543 Strain fitness (log2 ratio) -1
0
1
2 at 540.255 kb on + strand at 540.275 kb on + strand at 540.276 kb on + strand at 540.430 kb on - strand, within Atu3489 at 540.447 kb on + strand, within Atu3489 at 540.602 kb on + strand, within Atu3489 at 540.638 kb on + strand, within Atu3489 at 540.649 kb on - strand, within Atu3489 at 540.685 kb on + strand, within Atu3489 at 540.718 kb on - strand, within Atu3489 at 540.732 kb on + strand, within Atu3489 at 540.733 kb on - strand, within Atu3489 at 540.763 kb on - strand, within Atu3489 at 541.011 kb on + strand, within Atu3489 at 541.020 kb on - strand, within Atu3489 at 541.137 kb on + strand at 541.164 kb on - strand at 541.169 kb on - strand at 541.264 kb on - strand at 541.377 kb on - strand, within Atu3490 at 541.377 kb on - strand, within Atu3490 at 541.377 kb on - strand, within Atu3490 at 541.422 kb on - strand, within Atu3490 at 541.422 kb on - strand, within Atu3490 at 541.462 kb on + strand, within Atu3490 at 541.492 kb on + strand, within Atu3490 at 541.500 kb on - strand, within Atu3490 at 541.500 kb on - strand, within Atu3490 at 541.505 kb on - strand, within Atu3490 at 541.511 kb on + strand, within Atu3490 at 541.516 kb on + strand, within Atu3490 at 541.519 kb on - strand, within Atu3490 at 541.519 kb on - strand, within Atu3490 at 541.603 kb on + strand, within Atu3490 at 541.619 kb on + strand, within Atu3490 at 541.647 kb on - strand, within Atu3490 at 541.715 kb on - strand, within Atu3490 at 541.715 kb on - strand, within Atu3490 at 541.806 kb on + strand, within Atu3490 at 541.813 kb on - strand, within Atu3490 at 541.814 kb on - strand, within Atu3490 at 541.814 kb on - strand, within Atu3490 at 541.902 kb on - strand, within Atu3490 at 542.150 kb on - strand at 542.184 kb on + strand at 542.193 kb on + strand at 542.193 kb on + strand at 542.201 kb on - strand at 542.201 kb on - strand at 542.278 kb on + strand, within Atu3491 at 542.296 kb on - strand, within Atu3491 at 542.341 kb on + strand, within Atu3491 at 542.384 kb on + strand, within Atu3491 at 542.502 kb on + strand at 542.533 kb on + strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.541 kb on - strand at 542.587 kb on - strand at 542.660 kb on + strand at 542.926 kb on - strand, within Atu3492 at 542.985 kb on + strand, within Atu3492 at 543.061 kb on + strand, within Atu3492 at 543.071 kb on + strand, within Atu3492
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 540,255 + -0.1 540,275 + -0.2 540,276 + +0.5 540,430 - Atu3489 0.24 +0.0 540,447 + Atu3489 0.26 +0.4 540,602 + Atu3489 0.41 +1.0 540,638 + Atu3489 0.45 -0.2 540,649 - Atu3489 0.46 -0.4 540,685 + Atu3489 0.49 -0.8 540,718 - Atu3489 0.53 +0.5 540,732 + Atu3489 0.54 +0.5 540,733 - Atu3489 0.54 -0.2 540,763 - Atu3489 0.57 +0.5 541,011 + Atu3489 0.82 +0.1 541,020 - Atu3489 0.83 +0.1 541,137 + +0.1 541,164 - -0.5 541,169 - -0.7 541,264 - +0.7 541,377 - Atu3490 0.19 +0.2 541,377 - Atu3490 0.19 +0.0 541,377 - Atu3490 0.19 -0.7 541,422 - Atu3490 0.23 +0.9 541,422 - Atu3490 0.23 +0.7 541,462 + Atu3490 0.27 +0.0 541,492 + Atu3490 0.30 +0.5 541,500 - Atu3490 0.31 +1.0 541,500 - Atu3490 0.31 +0.7 541,505 - Atu3490 0.31 +2.5 541,511 + Atu3490 0.32 -0.8 541,516 + Atu3490 0.32 +0.0 541,519 - Atu3490 0.33 -0.0 541,519 - Atu3490 0.33 +1.0 541,603 + Atu3490 0.41 +0.1 541,619 + Atu3490 0.43 -0.2 541,647 - Atu3490 0.45 -0.1 541,715 - Atu3490 0.52 -0.3 541,715 - Atu3490 0.52 +1.3 541,806 + Atu3490 0.61 +2.6 541,813 - Atu3490 0.62 +0.1 541,814 - Atu3490 0.62 -0.1 541,814 - Atu3490 0.62 -1.3 541,902 - Atu3490 0.71 -0.4 542,150 - -0.2 542,184 + +0.4 542,193 + -0.2 542,193 + +0.0 542,201 - +0.8 542,201 - +0.6 542,278 + Atu3491 0.23 +0.0 542,296 - Atu3491 0.29 +0.2 542,341 + Atu3491 0.43 -0.0 542,384 + Atu3491 0.56 -0.3 542,502 + -1.2 542,533 + +0.1 542,541 - -0.9 542,541 - -1.1 542,541 - +0.0 542,541 - -0.1 542,541 - -0.1 542,541 - +1.1 542,587 - +0.3 542,660 + +0.2 542,926 - Atu3492 0.29 +0.6 542,985 + Atu3492 0.33 -0.2 543,061 + Atu3492 0.39 +0.1 543,071 + Atu3492 0.40 +0.9
Or see this region's nucleotide sequence