Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3247 and Atu3248 overlap by 4 nucleotides Atu3248 and Atu3249 are separated by 35 nucleotides Atu3249 and Atu3250 are separated by 62 nucleotides Atu3250 and Atu3251 are separated by 113 nucleotides
Atu3247: Atu3247 - cell division topological specificity factor MinE, at 265,951 to 266,211
Atu3247
Atu3248: Atu3248 - cell division inhibitor, at 266,208 to 267,023
Atu3248
Atu3249: Atu3249 - septum formation inhibitor, at 267,059 to 267,778
Atu3249
Atu3250: Atu3250 - hypothetical protein, at 267,841 to 268,083
Atu3250
Atu3251: Atu3251 - haloacid dehalogenase, at 268,197 to 268,883
Atu3251
Position (kb) 267
268 Strain fitness (log2 ratio) -1
0
1 at 266.258 kb on - strand at 266.313 kb on + strand, within Atu3248 at 266.378 kb on + strand, within Atu3248 at 266.396 kb on + strand, within Atu3248 at 266.406 kb on + strand, within Atu3248 at 266.409 kb on - strand, within Atu3248 at 266.577 kb on - strand, within Atu3248 at 266.587 kb on - strand, within Atu3248 at 266.636 kb on + strand, within Atu3248 at 266.682 kb on + strand, within Atu3248 at 266.740 kb on + strand, within Atu3248 at 266.834 kb on - strand, within Atu3248 at 266.847 kb on + strand, within Atu3248 at 266.872 kb on - strand, within Atu3248 at 266.880 kb on - strand, within Atu3248 at 266.917 kb on + strand, within Atu3248 at 266.917 kb on + strand, within Atu3248 at 266.925 kb on - strand, within Atu3248 at 267.027 kb on + strand at 267.028 kb on - strand at 267.032 kb on + strand at 267.032 kb on + strand at 267.040 kb on - strand at 267.040 kb on - strand at 267.155 kb on - strand, within Atu3249 at 267.263 kb on - strand, within Atu3249 at 267.263 kb on - strand, within Atu3249 at 267.347 kb on - strand, within Atu3249 at 267.383 kb on + strand, within Atu3249 at 267.454 kb on + strand, within Atu3249 at 267.646 kb on + strand, within Atu3249 at 267.654 kb on - strand, within Atu3249 at 267.658 kb on - strand, within Atu3249 at 267.966 kb on + strand, within Atu3250 at 267.966 kb on + strand, within Atu3250 at 268.176 kb on - strand at 268.264 kb on - strand at 268.285 kb on - strand, within Atu3251 at 268.291 kb on + strand, within Atu3251 at 268.341 kb on - strand, within Atu3251 at 268.394 kb on - strand, within Atu3251 at 268.461 kb on - strand, within Atu3251 at 268.541 kb on - strand, within Atu3251 at 268.550 kb on + strand, within Atu3251 at 268.593 kb on - strand, within Atu3251 at 268.633 kb on - strand, within Atu3251 at 268.686 kb on + strand, within Atu3251 at 268.694 kb on - strand, within Atu3251 at 268.740 kb on + strand, within Atu3251 at 268.750 kb on - strand, within Atu3251 at 268.750 kb on - strand, within Atu3251 at 268.777 kb on + strand, within Atu3251
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 266,258 - -0.4 266,313 + Atu3248 0.13 -0.4 266,378 + Atu3248 0.21 +0.1 266,396 + Atu3248 0.23 +0.2 266,406 + Atu3248 0.24 +0.2 266,409 - Atu3248 0.25 -0.5 266,577 - Atu3248 0.45 -0.3 266,587 - Atu3248 0.46 +1.4 266,636 + Atu3248 0.52 -0.5 266,682 + Atu3248 0.58 -0.1 266,740 + Atu3248 0.65 +0.6 266,834 - Atu3248 0.77 +0.1 266,847 + Atu3248 0.78 +0.3 266,872 - Atu3248 0.81 -0.0 266,880 - Atu3248 0.82 -0.2 266,917 + Atu3248 0.87 -0.1 266,917 + Atu3248 0.87 +0.0 266,925 - Atu3248 0.88 +0.6 267,027 + -1.7 267,028 - -0.5 267,032 + +0.5 267,032 + -0.2 267,040 - +0.2 267,040 - -0.4 267,155 - Atu3249 0.13 -0.6 267,263 - Atu3249 0.28 -0.2 267,263 - Atu3249 0.28 +0.2 267,347 - Atu3249 0.40 -0.3 267,383 + Atu3249 0.45 +0.2 267,454 + Atu3249 0.55 -0.7 267,646 + Atu3249 0.82 -0.1 267,654 - Atu3249 0.83 -0.7 267,658 - Atu3249 0.83 +0.4 267,966 + Atu3250 0.51 -0.0 267,966 + Atu3250 0.51 -0.5 268,176 - -0.2 268,264 - +0.3 268,285 - Atu3251 0.13 +0.2 268,291 + Atu3251 0.14 +0.2 268,341 - Atu3251 0.21 +0.2 268,394 - Atu3251 0.29 +0.1 268,461 - Atu3251 0.38 +0.4 268,541 - Atu3251 0.50 -0.5 268,550 + Atu3251 0.51 +0.6 268,593 - Atu3251 0.58 -0.1 268,633 - Atu3251 0.63 -0.6 268,686 + Atu3251 0.71 +0.2 268,694 - Atu3251 0.72 +0.7 268,740 + Atu3251 0.79 +0.7 268,750 - Atu3251 0.80 -0.5 268,750 - Atu3251 0.80 -0.9 268,777 + Atu3251 0.84 -0.1
Or see this region's nucleotide sequence