Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3171 and Atu3172 are separated by 4 nucleotides Atu3172 and Atu3173 are separated by 3 nucleotides
Atu3171: Atu3171 - sugar ABC transporter ATPase, at 180,955 to 182,031
Atu3171
Atu3172: Atu3172 - sugar ABC transporter permease, at 182,036 to 182,866
Atu3172
Atu3173: Atu3173 - sugar ABC transporter permease, at 182,870 to 183,754
Atu3173
Position (kb) 182
183 Strain fitness (log2 ratio) -1
0
1
2 at 181.083 kb on - strand, within Atu3171 at 181.107 kb on + strand, within Atu3171 at 181.179 kb on + strand, within Atu3171 at 181.222 kb on + strand, within Atu3171 at 181.392 kb on + strand, within Atu3171 at 181.398 kb on - strand, within Atu3171 at 181.450 kb on - strand, within Atu3171 at 181.452 kb on + strand, within Atu3171 at 181.458 kb on + strand, within Atu3171 at 181.543 kb on - strand, within Atu3171 at 181.667 kb on - strand, within Atu3171 at 181.742 kb on - strand, within Atu3171 at 181.769 kb on + strand, within Atu3171 at 181.820 kb on - strand, within Atu3171 at 181.972 kb on + strand at 181.989 kb on + strand at 182.033 kb on + strand at 182.033 kb on + strand at 182.073 kb on + strand at 182.133 kb on + strand, within Atu3172 at 182.360 kb on + strand, within Atu3172 at 182.367 kb on - strand, within Atu3172 at 182.367 kb on - strand, within Atu3172 at 182.420 kb on + strand, within Atu3172 at 182.428 kb on - strand, within Atu3172 at 182.438 kb on - strand, within Atu3172 at 182.438 kb on - strand, within Atu3172 at 182.563 kb on - strand, within Atu3172 at 182.586 kb on + strand, within Atu3172 at 182.623 kb on + strand, within Atu3172 at 182.722 kb on + strand, within Atu3172 at 182.742 kb on + strand, within Atu3172 at 182.790 kb on + strand at 182.862 kb on + strand at 182.868 kb on + strand at 182.868 kb on + strand at 182.911 kb on + strand at 182.919 kb on - strand at 182.924 kb on - strand at 182.924 kb on - strand at 182.924 kb on - strand at 183.206 kb on + strand, within Atu3173 at 183.207 kb on + strand, within Atu3173 at 183.345 kb on - strand, within Atu3173 at 183.345 kb on - strand, within Atu3173 at 183.377 kb on - strand, within Atu3173 at 183.379 kb on + strand, within Atu3173 at 183.417 kb on + strand, within Atu3173 at 183.425 kb on - strand, within Atu3173 at 183.443 kb on + strand, within Atu3173 at 183.528 kb on + strand, within Atu3173 at 183.648 kb on + strand, within Atu3173 at 183.648 kb on + strand, within Atu3173 at 183.648 kb on + strand, within Atu3173 at 183.648 kb on + strand, within Atu3173 at 183.656 kb on - strand, within Atu3173 at 183.656 kb on - strand, within Atu3173 at 183.722 kb on - strand at 183.722 kb on - strand at 183.726 kb on + strand at 183.733 kb on - strand at 183.802 kb on + strand at 183.817 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 181,083 - Atu3171 0.12 -0.4 181,107 + Atu3171 0.14 +0.5 181,179 + Atu3171 0.21 +0.2 181,222 + Atu3171 0.25 -0.2 181,392 + Atu3171 0.41 -0.3 181,398 - Atu3171 0.41 +0.2 181,450 - Atu3171 0.46 -0.2 181,452 + Atu3171 0.46 +0.1 181,458 + Atu3171 0.47 +0.7 181,543 - Atu3171 0.55 -0.2 181,667 - Atu3171 0.66 +0.7 181,742 - Atu3171 0.73 +1.3 181,769 + Atu3171 0.76 +0.1 181,820 - Atu3171 0.80 +0.3 181,972 + +0.0 181,989 + +0.0 182,033 + +0.7 182,033 + +0.2 182,073 + -0.2 182,133 + Atu3172 0.12 +0.1 182,360 + Atu3172 0.39 +0.3 182,367 - Atu3172 0.40 -0.1 182,367 - Atu3172 0.40 -1.2 182,420 + Atu3172 0.46 +1.0 182,428 - Atu3172 0.47 +0.2 182,438 - Atu3172 0.48 +0.4 182,438 - Atu3172 0.48 -0.2 182,563 - Atu3172 0.63 -0.2 182,586 + Atu3172 0.66 +0.1 182,623 + Atu3172 0.71 +0.1 182,722 + Atu3172 0.83 +0.3 182,742 + Atu3172 0.85 +0.8 182,790 + +0.1 182,862 + +0.5 182,868 + -0.4 182,868 + -0.3 182,911 + +0.1 182,919 - -0.6 182,924 - +0.3 182,924 - +1.2 182,924 - -0.1 183,206 + Atu3173 0.38 +0.2 183,207 + Atu3173 0.38 +0.7 183,345 - Atu3173 0.54 +0.7 183,345 - Atu3173 0.54 +0.5 183,377 - Atu3173 0.57 +0.2 183,379 + Atu3173 0.58 +0.1 183,417 + Atu3173 0.62 -0.4 183,425 - Atu3173 0.63 +0.1 183,443 + Atu3173 0.65 +0.6 183,528 + Atu3173 0.74 -0.6 183,648 + Atu3173 0.88 -0.5 183,648 + Atu3173 0.88 +0.2 183,648 + Atu3173 0.88 +0.8 183,648 + Atu3173 0.88 -1.0 183,656 - Atu3173 0.89 +0.0 183,656 - Atu3173 0.89 +2.0 183,722 - +1.6 183,722 - +0.0 183,726 + +0.3 183,733 - -0.5 183,802 + -0.6 183,817 - +0.7
Or see this region's nucleotide sequence