Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2647 and Atu2648 are separated by 7 nucleotides Atu2648 and Atu2649 are separated by 6 nucleotides Atu2649 and Atu2650 are separated by 87 nucleotides Atu2650 and Atu2651 overlap by 1 nucleotides
Atu2647: Atu2647 - hypothetical protein, at 2,632,044 to 2,632,688
Atu2647
Atu2648: Atu2648 - hypothetical protein, at 2,632,696 to 2,633,139
Atu2648
Atu2649: Atu2649 - hypothetical protein, at 2,633,146 to 2,633,439
Atu2649
Atu2650: Atu2650 - glycerol-3-phosphate dehydrogenase, at 2,633,527 to 2,634,510
Atu2650
Atu2651: Atu2651 - O-sialoglycoprotein endopeptidase, at 2,634,510 to 2,635,607
Atu2651
Position (kb)
2633
2634
2635 Strain fitness (log2 ratio)
-2
-1
0
1 at 2632.529 kb on - strand, within Atu2647 at 2632.536 kb on - strand, within Atu2647 at 2632.580 kb on + strand, within Atu2647 at 2632.588 kb on - strand, within Atu2647 at 2632.675 kb on + strand at 2632.783 kb on - strand, within Atu2648 at 2632.807 kb on - strand, within Atu2648 at 2632.883 kb on + strand, within Atu2648 at 2632.959 kb on - strand, within Atu2648 at 2632.967 kb on - strand, within Atu2648 at 2632.984 kb on + strand, within Atu2648 at 2633.072 kb on + strand, within Atu2648 at 2633.080 kb on - strand, within Atu2648 at 2633.098 kb on - strand at 2633.109 kb on + strand at 2633.109 kb on + strand at 2633.114 kb on + strand at 2633.114 kb on + strand at 2633.122 kb on - strand at 2633.161 kb on + strand at 2633.195 kb on + strand, within Atu2649 at 2633.195 kb on + strand, within Atu2649 at 2633.195 kb on + strand, within Atu2649 at 2633.205 kb on + strand, within Atu2649 at 2633.370 kb on - strand, within Atu2649 at 2633.416 kb on - strand at 2633.449 kb on - strand at 2633.474 kb on + strand at 2633.565 kb on - strand at 2633.575 kb on - strand at 2633.686 kb on - strand, within Atu2650 at 2633.903 kb on - strand, within Atu2650 at 2633.948 kb on + strand, within Atu2650 at 2633.948 kb on + strand, within Atu2650 at 2633.948 kb on + strand, within Atu2650 at 2633.963 kb on - strand, within Atu2650 at 2634.038 kb on + strand, within Atu2650 at 2634.111 kb on + strand, within Atu2650 at 2634.119 kb on - strand, within Atu2650 at 2634.138 kb on - strand, within Atu2650 at 2634.235 kb on + strand, within Atu2650 at 2634.235 kb on + strand, within Atu2650 at 2634.243 kb on - strand, within Atu2650 at 2634.243 kb on - strand, within Atu2650 at 2634.243 kb on - strand, within Atu2650 at 2634.303 kb on + strand, within Atu2650 at 2634.340 kb on + strand, within Atu2650 at 2634.340 kb on + strand, within Atu2650 at 2634.348 kb on - strand, within Atu2650 at 2634.364 kb on + strand, within Atu2650 at 2634.464 kb on + strand at 2634.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,632,529 - Atu2647 0.75 +0.8 2,632,536 - Atu2647 0.76 +0.2 2,632,580 + Atu2647 0.83 -0.0 2,632,588 - Atu2647 0.84 -0.1 2,632,675 + -0.1 2,632,783 - Atu2648 0.20 -1.8 2,632,807 - Atu2648 0.25 -2.5 2,632,883 + Atu2648 0.42 -0.5 2,632,959 - Atu2648 0.59 +0.1 2,632,967 - Atu2648 0.61 -2.4 2,632,984 + Atu2648 0.65 +0.5 2,633,072 + Atu2648 0.85 -1.7 2,633,080 - Atu2648 0.86 -1.5 2,633,098 - -2.3 2,633,109 + -1.3 2,633,109 + +0.7 2,633,114 + -0.5 2,633,114 + +1.3 2,633,122 - -1.3 2,633,161 + -0.2 2,633,195 + Atu2649 0.17 -0.3 2,633,195 + Atu2649 0.17 -0.8 2,633,195 + Atu2649 0.17 +0.4 2,633,205 + Atu2649 0.20 -0.1 2,633,370 - Atu2649 0.76 -0.8 2,633,416 - -0.2 2,633,449 - +0.2 2,633,474 + +0.5 2,633,565 - -1.1 2,633,575 - -0.2 2,633,686 - Atu2650 0.16 -1.0 2,633,903 - Atu2650 0.38 +1.1 2,633,948 + Atu2650 0.43 -1.0 2,633,948 + Atu2650 0.43 -1.2 2,633,948 + Atu2650 0.43 -1.3 2,633,963 - Atu2650 0.44 -0.6 2,634,038 + Atu2650 0.52 +0.1 2,634,111 + Atu2650 0.59 -1.0 2,634,119 - Atu2650 0.60 -0.0 2,634,138 - Atu2650 0.62 -0.4 2,634,235 + Atu2650 0.72 -1.6 2,634,235 + Atu2650 0.72 +0.6 2,634,243 - Atu2650 0.73 -0.7 2,634,243 - Atu2650 0.73 -1.0 2,634,243 - Atu2650 0.73 -1.0 2,634,303 + Atu2650 0.79 -1.3 2,634,340 + Atu2650 0.83 -0.6 2,634,340 + Atu2650 0.83 -0.7 2,634,348 - Atu2650 0.83 -0.9 2,634,364 + Atu2650 0.85 +0.5 2,634,464 + -0.3 2,634,464 + -1.1
Or see this region's nucleotide sequence