Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2288 and Atu2289 are separated by 282 nucleotides Atu2289 and Atu2290 overlap by 1 nucleotides Atu2290 and Atu2291 overlap by 4 nucleotides
Atu2288: Atu2288 - hypothetical protein, at 2,260,960 to 2,261,862
Atu2288
Atu2289: Atu2289 - anthranilate synthase component I and II, at 2,262,145 to 2,264,334
Atu2289
Atu2290: Atu2290 - probable acetyltransferase, at 2,264,334 to 2,264,843
Atu2290
Atu2291: Atu2291 - hypothetical protein, at 2,264,840 to 2,265,787
Atu2291
Position (kb)
2262
2263
2264
2265 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2261.230 kb on - strand, within Atu2288 at 2261.239 kb on + strand, within Atu2288 at 2261.247 kb on - strand, within Atu2288 at 2261.334 kb on + strand, within Atu2288 at 2261.353 kb on - strand, within Atu2288 at 2261.394 kb on - strand, within Atu2288 at 2261.399 kb on - strand, within Atu2288 at 2261.399 kb on - strand, within Atu2288 at 2261.399 kb on - strand, within Atu2288 at 2261.428 kb on + strand, within Atu2288 at 2261.428 kb on + strand, within Atu2288 at 2261.431 kb on - strand, within Atu2288 at 2261.431 kb on - strand, within Atu2288 at 2261.444 kb on - strand, within Atu2288 at 2261.500 kb on + strand, within Atu2288 at 2261.518 kb on + strand, within Atu2288 at 2261.583 kb on + strand, within Atu2288 at 2261.601 kb on - strand, within Atu2288 at 2261.686 kb on - strand, within Atu2288 at 2261.755 kb on + strand, within Atu2288 at 2261.771 kb on + strand, within Atu2288 at 2261.838 kb on + strand at 2261.859 kb on + strand at 2261.869 kb on + strand at 2261.874 kb on + strand at 2261.881 kb on - strand at 2261.881 kb on - strand at 2261.895 kb on + strand at 2262.108 kb on + strand at 2262.117 kb on + strand at 2262.125 kb on - strand at 2262.172 kb on + strand at 2262.172 kb on + strand at 2262.209 kb on + strand at 2262.389 kb on - strand, within Atu2289 at 2262.402 kb on - strand, within Atu2289 at 2262.458 kb on + strand, within Atu2289 at 2262.488 kb on + strand, within Atu2289 at 2262.564 kb on - strand, within Atu2289 at 2262.721 kb on + strand, within Atu2289 at 2262.739 kb on + strand, within Atu2289 at 2262.756 kb on + strand, within Atu2289 at 2262.756 kb on + strand, within Atu2289 at 2262.756 kb on + strand, within Atu2289 at 2262.764 kb on - strand, within Atu2289 at 2262.764 kb on - strand, within Atu2289 at 2262.764 kb on - strand, within Atu2289 at 2262.814 kb on + strand, within Atu2289 at 2262.842 kb on - strand, within Atu2289 at 2262.908 kb on - strand, within Atu2289 at 2262.926 kb on + strand, within Atu2289 at 2262.936 kb on + strand, within Atu2289 at 2262.944 kb on - strand, within Atu2289 at 2263.004 kb on + strand, within Atu2289 at 2263.034 kb on - strand, within Atu2289 at 2263.127 kb on - strand, within Atu2289 at 2263.147 kb on - strand, within Atu2289 at 2263.147 kb on - strand, within Atu2289 at 2263.242 kb on - strand, within Atu2289 at 2263.257 kb on - strand, within Atu2289 at 2263.326 kb on - strand, within Atu2289 at 2263.331 kb on + strand, within Atu2289 at 2263.331 kb on + strand, within Atu2289 at 2263.373 kb on + strand, within Atu2289 at 2263.403 kb on - strand, within Atu2289 at 2263.403 kb on - strand, within Atu2289 at 2263.643 kb on + strand, within Atu2289 at 2263.762 kb on + strand, within Atu2289 at 2263.840 kb on - strand, within Atu2289 at 2263.851 kb on + strand, within Atu2289 at 2263.915 kb on + strand, within Atu2289 at 2263.978 kb on - strand, within Atu2289 at 2263.989 kb on + strand, within Atu2289 at 2264.012 kb on + strand, within Atu2289 at 2264.020 kb on - strand, within Atu2289 at 2264.031 kb on + strand, within Atu2289 at 2264.045 kb on - strand, within Atu2289 at 2264.105 kb on + strand, within Atu2289 at 2264.113 kb on - strand, within Atu2289 at 2264.113 kb on - strand, within Atu2289 at 2264.184 kb on + strand at 2264.224 kb on + strand at 2264.229 kb on + strand at 2264.262 kb on + strand at 2264.298 kb on - strand at 2264.319 kb on + strand at 2264.319 kb on + strand at 2264.319 kb on + strand at 2264.321 kb on - strand at 2264.327 kb on - strand at 2264.344 kb on + strand at 2264.376 kb on - strand at 2264.381 kb on + strand at 2264.677 kb on + strand, within Atu2290 at 2264.692 kb on + strand, within Atu2290 at 2264.700 kb on - strand, within Atu2290 at 2264.720 kb on + strand, within Atu2290 at 2264.723 kb on - strand, within Atu2290 at 2264.728 kb on - strand, within Atu2290 at 2264.728 kb on - strand, within Atu2290 at 2264.811 kb on + strand at 2264.847 kb on + strand at 2264.922 kb on + strand at 2264.927 kb on + strand at 2264.946 kb on + strand, within Atu2291 at 2264.954 kb on - strand, within Atu2291 at 2264.954 kb on - strand, within Atu2291 at 2264.967 kb on - strand, within Atu2291 at 2265.198 kb on + strand, within Atu2291 at 2265.201 kb on - strand, within Atu2291 at 2265.201 kb on - strand, within Atu2291 at 2265.203 kb on + strand, within Atu2291
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,261,230 - Atu2288 0.30 +0.2 2,261,239 + Atu2288 0.31 +0.9 2,261,247 - Atu2288 0.32 +0.5 2,261,334 + Atu2288 0.41 -0.0 2,261,353 - Atu2288 0.44 +0.8 2,261,394 - Atu2288 0.48 -0.6 2,261,399 - Atu2288 0.49 +0.2 2,261,399 - Atu2288 0.49 +0.3 2,261,399 - Atu2288 0.49 -0.1 2,261,428 + Atu2288 0.52 +0.1 2,261,428 + Atu2288 0.52 +0.1 2,261,431 - Atu2288 0.52 -0.1 2,261,431 - Atu2288 0.52 -0.3 2,261,444 - Atu2288 0.54 +0.9 2,261,500 + Atu2288 0.60 -0.2 2,261,518 + Atu2288 0.62 +0.1 2,261,583 + Atu2288 0.69 +1.0 2,261,601 - Atu2288 0.71 -0.2 2,261,686 - Atu2288 0.80 -0.4 2,261,755 + Atu2288 0.88 -0.5 2,261,771 + Atu2288 0.90 -0.1 2,261,838 + +0.2 2,261,859 + +0.5 2,261,869 + +0.5 2,261,874 + -0.3 2,261,881 - -0.0 2,261,881 - -0.2 2,261,895 + -0.4 2,262,108 + -0.5 2,262,117 + -0.2 2,262,125 - +1.4 2,262,172 + -1.3 2,262,172 + -0.4 2,262,209 + +0.1 2,262,389 - Atu2289 0.11 -0.6 2,262,402 - Atu2289 0.12 -0.7 2,262,458 + Atu2289 0.14 -1.2 2,262,488 + Atu2289 0.16 -1.2 2,262,564 - Atu2289 0.19 -3.6 2,262,721 + Atu2289 0.26 -1.8 2,262,739 + Atu2289 0.27 -0.5 2,262,756 + Atu2289 0.28 -1.1 2,262,756 + Atu2289 0.28 -1.6 2,262,756 + Atu2289 0.28 -2.1 2,262,764 - Atu2289 0.28 -0.9 2,262,764 - Atu2289 0.28 -1.6 2,262,764 - Atu2289 0.28 +0.1 2,262,814 + Atu2289 0.31 -2.3 2,262,842 - Atu2289 0.32 -1.7 2,262,908 - Atu2289 0.35 -1.1 2,262,926 + Atu2289 0.36 +0.5 2,262,936 + Atu2289 0.36 -1.6 2,262,944 - Atu2289 0.36 +0.7 2,263,004 + Atu2289 0.39 -1.8 2,263,034 - Atu2289 0.41 -1.1 2,263,127 - Atu2289 0.45 -0.3 2,263,147 - Atu2289 0.46 -2.3 2,263,147 - Atu2289 0.46 -1.0 2,263,242 - Atu2289 0.50 -1.8 2,263,257 - Atu2289 0.51 -1.0 2,263,326 - Atu2289 0.54 -2.1 2,263,331 + Atu2289 0.54 -2.1 2,263,331 + Atu2289 0.54 -0.8 2,263,373 + Atu2289 0.56 -1.6 2,263,403 - Atu2289 0.57 -1.0 2,263,403 - Atu2289 0.57 -1.1 2,263,643 + Atu2289 0.68 -1.2 2,263,762 + Atu2289 0.74 +1.5 2,263,840 - Atu2289 0.77 -1.5 2,263,851 + Atu2289 0.78 -1.4 2,263,915 + Atu2289 0.81 -1.1 2,263,978 - Atu2289 0.84 -1.4 2,263,989 + Atu2289 0.84 -0.7 2,264,012 + Atu2289 0.85 -1.5 2,264,020 - Atu2289 0.86 -1.5 2,264,031 + Atu2289 0.86 -2.8 2,264,045 - Atu2289 0.87 +0.1 2,264,105 + Atu2289 0.89 -0.9 2,264,113 - Atu2289 0.90 -1.2 2,264,113 - Atu2289 0.90 +0.5 2,264,184 + -2.1 2,264,224 + -3.5 2,264,229 + -2.3 2,264,262 + -2.5 2,264,298 - -0.1 2,264,319 + +0.8 2,264,319 + +0.1 2,264,319 + +0.3 2,264,321 - +0.4 2,264,327 - +0.1 2,264,344 + +0.0 2,264,376 - +0.2 2,264,381 + +0.5 2,264,677 + Atu2290 0.67 +0.0 2,264,692 + Atu2290 0.70 -0.6 2,264,700 - Atu2290 0.72 -0.5 2,264,720 + Atu2290 0.76 -0.0 2,264,723 - Atu2290 0.76 -0.2 2,264,728 - Atu2290 0.77 +0.0 2,264,728 - Atu2290 0.77 -0.5 2,264,811 + +0.8 2,264,847 + +0.1 2,264,922 + -0.4 2,264,927 + +0.2 2,264,946 + Atu2291 0.11 +0.2 2,264,954 - Atu2291 0.12 -0.0 2,264,954 - Atu2291 0.12 -1.1 2,264,967 - Atu2291 0.13 +0.0 2,265,198 + Atu2291 0.38 -1.6 2,265,201 - Atu2291 0.38 -0.8 2,265,201 - Atu2291 0.38 +0.3 2,265,203 + Atu2291 0.38 -0.3
Or see this region's nucleotide sequence