Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2060 and Atu2061 overlap by 4 nucleotides Atu2061 and Atu2062 are separated by 119 nucleotides Atu2062 and Atu2063 overlap by 4 nucleotides
Atu2060: Atu2060 - ABC transporter, substrate binding protein (glycine betaine), at 2,024,037 to 2,024,963
Atu2060
Atu2061: Atu2061 - hydrolase, at 2,024,960 to 2,025,820
Atu2061
Atu2062: Atu2062 - hypothetical protein, at 2,025,940 to 2,026,398
Atu2062
Atu2063: Atu2063 - hypothetical protein, at 2,026,395 to 2,026,820
Atu2063
Position (kb)
2024
2025
2026 Strain fitness (log2 ratio)
-1
0
1 at 2024.116 kb on - strand at 2024.169 kb on + strand, within Atu2060 at 2024.177 kb on - strand, within Atu2060 at 2024.205 kb on - strand, within Atu2060 at 2024.239 kb on - strand, within Atu2060 at 2024.239 kb on - strand, within Atu2060 at 2024.239 kb on - strand, within Atu2060 at 2024.241 kb on + strand, within Atu2060 at 2024.302 kb on + strand, within Atu2060 at 2024.349 kb on + strand, within Atu2060 at 2024.528 kb on + strand, within Atu2060 at 2024.530 kb on + strand, within Atu2060 at 2024.530 kb on + strand, within Atu2060 at 2024.536 kb on - strand, within Atu2060 at 2024.538 kb on - strand, within Atu2060 at 2024.734 kb on + strand, within Atu2060 at 2024.861 kb on + strand, within Atu2060 at 2024.956 kb on + strand at 2024.956 kb on + strand at 2024.956 kb on + strand at 2024.956 kb on + strand at 2024.964 kb on - strand at 2024.964 kb on - strand at 2024.964 kb on - strand at 2025.059 kb on - strand, within Atu2061 at 2025.147 kb on + strand, within Atu2061 at 2025.169 kb on - strand, within Atu2061 at 2025.271 kb on + strand, within Atu2061 at 2025.271 kb on + strand, within Atu2061 at 2025.313 kb on + strand, within Atu2061 at 2025.315 kb on + strand, within Atu2061 at 2025.324 kb on + strand, within Atu2061 at 2025.340 kb on + strand, within Atu2061 at 2025.343 kb on - strand, within Atu2061 at 2025.348 kb on - strand, within Atu2061 at 2025.487 kb on - strand, within Atu2061 at 2025.538 kb on + strand, within Atu2061 at 2025.571 kb on - strand, within Atu2061 at 2025.573 kb on - strand, within Atu2061 at 2025.681 kb on - strand, within Atu2061 at 2025.803 kb on + strand at 2025.913 kb on + strand at 2026.054 kb on - strand, within Atu2062 at 2026.086 kb on - strand, within Atu2062 at 2026.091 kb on - strand, within Atu2062 at 2026.154 kb on - strand, within Atu2062 at 2026.154 kb on - strand, within Atu2062 at 2026.235 kb on - strand, within Atu2062 at 2026.243 kb on - strand, within Atu2062 at 2026.255 kb on - strand, within Atu2062 at 2026.324 kb on + strand, within Atu2062 at 2026.324 kb on - strand, within Atu2062 at 2026.346 kb on + strand, within Atu2062 at 2026.346 kb on + strand, within Atu2062 at 2026.354 kb on - strand at 2026.361 kb on + strand at 2026.410 kb on + strand at 2026.418 kb on - strand at 2026.483 kb on - strand, within Atu2063 at 2026.483 kb on - strand, within Atu2063
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 2,024,116 - +0.0 2,024,169 + Atu2060 0.14 +0.2 2,024,177 - Atu2060 0.15 +0.7 2,024,205 - Atu2060 0.18 +1.5 2,024,239 - Atu2060 0.22 +0.0 2,024,239 - Atu2060 0.22 -0.4 2,024,239 - Atu2060 0.22 +0.6 2,024,241 + Atu2060 0.22 +0.6 2,024,302 + Atu2060 0.29 +0.2 2,024,349 + Atu2060 0.34 +0.1 2,024,528 + Atu2060 0.53 -0.1 2,024,530 + Atu2060 0.53 -0.1 2,024,530 + Atu2060 0.53 -0.8 2,024,536 - Atu2060 0.54 -0.7 2,024,538 - Atu2060 0.54 +0.1 2,024,734 + Atu2060 0.75 -1.1 2,024,861 + Atu2060 0.89 +0.5 2,024,956 + +1.3 2,024,956 + -0.9 2,024,956 + -0.1 2,024,956 + +0.5 2,024,964 - +0.0 2,024,964 - +0.0 2,024,964 - -0.2 2,025,059 - Atu2061 0.11 +0.9 2,025,147 + Atu2061 0.22 -1.1 2,025,169 - Atu2061 0.24 -0.2 2,025,271 + Atu2061 0.36 -0.5 2,025,271 + Atu2061 0.36 +0.6 2,025,313 + Atu2061 0.41 +0.4 2,025,315 + Atu2061 0.41 +1.1 2,025,324 + Atu2061 0.42 -1.1 2,025,340 + Atu2061 0.44 -0.0 2,025,343 - Atu2061 0.44 +1.0 2,025,348 - Atu2061 0.45 +0.5 2,025,487 - Atu2061 0.61 +0.2 2,025,538 + Atu2061 0.67 +0.6 2,025,571 - Atu2061 0.71 +0.1 2,025,573 - Atu2061 0.71 -0.0 2,025,681 - Atu2061 0.84 +0.1 2,025,803 + -0.1 2,025,913 + +0.5 2,026,054 - Atu2062 0.25 -0.6 2,026,086 - Atu2062 0.32 -0.2 2,026,091 - Atu2062 0.33 +0.0 2,026,154 - Atu2062 0.47 +0.2 2,026,154 - Atu2062 0.47 -0.6 2,026,235 - Atu2062 0.64 -0.2 2,026,243 - Atu2062 0.66 +0.6 2,026,255 - Atu2062 0.69 -0.1 2,026,324 + Atu2062 0.84 +0.3 2,026,324 - Atu2062 0.84 +1.0 2,026,346 + Atu2062 0.88 +0.9 2,026,346 + Atu2062 0.88 +0.7 2,026,354 - +0.1 2,026,361 + +0.2 2,026,410 + -0.0 2,026,418 - -0.2 2,026,483 - Atu2063 0.21 -0.0 2,026,483 - Atu2063 0.21 +0.0
Or see this region's nucleotide sequence