Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1981 and Atu1982 are separated by 125 nucleotides Atu1982 and Atu1983 overlap by 4 nucleotides Atu1983 and Atu1984 are separated by 84 nucleotides
Atu1981: Atu1981 - hypothetical protein, at 1,947,376 to 1,947,807
Atu1981
Atu1982: Atu1982 - sigma factor, at 1,947,933 to 1,948,475
Atu1982
Atu1983: Atu1983 - hypothetical protein, at 1,948,472 to 1,949,182
Atu1983
Atu1984: Atu1984 - two component sensor kinase, at 1,949,267 to 1,950,307
Atu1984
Position (kb)
1948
1949
1950 Strain fitness (log2 ratio)
-2
-1
0
1 at 1947.528 kb on - strand, within Atu1981 at 1947.529 kb on + strand, within Atu1981 at 1947.534 kb on + strand, within Atu1981 at 1947.542 kb on + strand, within Atu1981 at 1947.574 kb on + strand, within Atu1981 at 1947.575 kb on - strand, within Atu1981 at 1947.601 kb on - strand, within Atu1981 at 1947.606 kb on + strand, within Atu1981 at 1947.614 kb on - strand, within Atu1981 at 1947.614 kb on - strand, within Atu1981 at 1947.644 kb on + strand, within Atu1981 at 1947.644 kb on + strand, within Atu1981 at 1947.644 kb on + strand, within Atu1981 at 1947.652 kb on - strand, within Atu1981 at 1947.675 kb on - strand, within Atu1981 at 1947.724 kb on - strand, within Atu1981 at 1947.821 kb on - strand at 1947.881 kb on + strand at 1947.938 kb on + strand at 1948.055 kb on + strand, within Atu1982 at 1948.065 kb on - strand, within Atu1982 at 1948.075 kb on - strand, within Atu1982 at 1948.152 kb on + strand, within Atu1982 at 1948.239 kb on - strand, within Atu1982 at 1948.250 kb on + strand, within Atu1982 at 1948.263 kb on - strand, within Atu1982 at 1948.300 kb on + strand, within Atu1982 at 1948.308 kb on - strand, within Atu1982 at 1948.318 kb on - strand, within Atu1982 at 1948.350 kb on + strand, within Atu1982 at 1948.373 kb on + strand, within Atu1982 at 1948.376 kb on - strand, within Atu1982 at 1948.378 kb on + strand, within Atu1982 at 1948.378 kb on + strand, within Atu1982 at 1948.402 kb on + strand, within Atu1982 at 1948.403 kb on - strand, within Atu1982 at 1948.460 kb on - strand at 1948.538 kb on - strand at 1948.676 kb on - strand, within Atu1983 at 1948.750 kb on - strand, within Atu1983 at 1948.770 kb on + strand, within Atu1983 at 1948.778 kb on - strand, within Atu1983 at 1948.984 kb on + strand, within Atu1983 at 1948.992 kb on - strand, within Atu1983 at 1949.111 kb on + strand, within Atu1983 at 1949.216 kb on + strand at 1949.243 kb on + strand at 1949.273 kb on - strand at 1949.331 kb on - strand at 1949.343 kb on - strand at 1949.349 kb on + strand at 1949.380 kb on - strand, within Atu1984 at 1949.531 kb on - strand, within Atu1984 at 1949.531 kb on - strand, within Atu1984 at 1949.642 kb on - strand, within Atu1984 at 1949.700 kb on + strand, within Atu1984 at 1949.700 kb on + strand, within Atu1984 at 1949.708 kb on - strand, within Atu1984 at 1949.708 kb on - strand, within Atu1984 at 1949.722 kb on + strand, within Atu1984 at 1949.937 kb on - strand, within Atu1984 at 1949.937 kb on - strand, within Atu1984 at 1950.041 kb on + strand, within Atu1984 at 1950.159 kb on + strand, within Atu1984
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,947,528 - Atu1981 0.35 -0.5 1,947,529 + Atu1981 0.35 +1.3 1,947,534 + Atu1981 0.37 +0.3 1,947,542 + Atu1981 0.38 -0.7 1,947,574 + Atu1981 0.46 +0.4 1,947,575 - Atu1981 0.46 +0.3 1,947,601 - Atu1981 0.52 +0.3 1,947,606 + Atu1981 0.53 +0.3 1,947,614 - Atu1981 0.55 -0.4 1,947,614 - Atu1981 0.55 -0.0 1,947,644 + Atu1981 0.62 +0.1 1,947,644 + Atu1981 0.62 -0.5 1,947,644 + Atu1981 0.62 +0.0 1,947,652 - Atu1981 0.64 +0.5 1,947,675 - Atu1981 0.69 -0.2 1,947,724 - Atu1981 0.81 +0.2 1,947,821 - -0.0 1,947,881 + -1.2 1,947,938 + -0.1 1,948,055 + Atu1982 0.22 +0.1 1,948,065 - Atu1982 0.24 +0.4 1,948,075 - Atu1982 0.26 +0.6 1,948,152 + Atu1982 0.40 +0.2 1,948,239 - Atu1982 0.56 -0.9 1,948,250 + Atu1982 0.58 +0.7 1,948,263 - Atu1982 0.61 +0.4 1,948,300 + Atu1982 0.68 +0.8 1,948,308 - Atu1982 0.69 -0.3 1,948,318 - Atu1982 0.71 -0.1 1,948,350 + Atu1982 0.77 -0.9 1,948,373 + Atu1982 0.81 +0.2 1,948,376 - Atu1982 0.82 -0.4 1,948,378 + Atu1982 0.82 +0.3 1,948,378 + Atu1982 0.82 -0.2 1,948,402 + Atu1982 0.86 -1.0 1,948,403 - Atu1982 0.87 -1.0 1,948,460 - -0.1 1,948,538 - +0.1 1,948,676 - Atu1983 0.29 -0.1 1,948,750 - Atu1983 0.39 -1.3 1,948,770 + Atu1983 0.42 -0.6 1,948,778 - Atu1983 0.43 -0.5 1,948,984 + Atu1983 0.72 -1.4 1,948,992 - Atu1983 0.73 -2.2 1,949,111 + Atu1983 0.90 -1.3 1,949,216 + +0.3 1,949,243 + +0.1 1,949,273 - -0.2 1,949,331 - -0.2 1,949,343 - +0.7 1,949,349 + -1.2 1,949,380 - Atu1984 0.11 -0.6 1,949,531 - Atu1984 0.25 +0.4 1,949,531 - Atu1984 0.25 +0.3 1,949,642 - Atu1984 0.36 -0.4 1,949,700 + Atu1984 0.42 -0.4 1,949,700 + Atu1984 0.42 +0.2 1,949,708 - Atu1984 0.42 -0.1 1,949,708 - Atu1984 0.42 +0.3 1,949,722 + Atu1984 0.44 +0.7 1,949,937 - Atu1984 0.64 +0.6 1,949,937 - Atu1984 0.64 +0.1 1,950,041 + Atu1984 0.74 +0.0 1,950,159 + Atu1984 0.86 -0.4
Or see this region's nucleotide sequence