Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1904 and Atu1905 are separated by 24 nucleotides Atu1905 and Atu1906 are separated by 169 nucleotides Atu1906 and Atu1907 overlap by 4 nucleotides
Atu1904: Atu1904 - transcriptional regulator, at 1,881,707 to 1,882,642
Atu1904
Atu1905: Atu1905 - short chain dehydrogenase, at 1,882,667 to 1,883,452
Atu1905
Atu1906: Atu1906 - ABC transporter, membrane spanning protein, at 1,883,622 to 1,884,383
Atu1906
Atu1907: Atu1907 - ABC transporter, nucleotide binding/ATPase protein, at 1,884,380 to 1,885,306
Atu1907
Position (kb)
1883
1884
1885 Strain fitness (log2 ratio)
-1
0
1 at 1882.729 kb on - strand at 1882.774 kb on - strand, within Atu1905 at 1882.794 kb on - strand, within Atu1905 at 1882.892 kb on - strand, within Atu1905 at 1882.926 kb on - strand, within Atu1905 at 1883.128 kb on + strand, within Atu1905 at 1883.136 kb on - strand, within Atu1905 at 1883.205 kb on - strand, within Atu1905 at 1883.318 kb on + strand, within Atu1905 at 1883.467 kb on + strand at 1883.472 kb on - strand at 1883.472 kb on - strand at 1883.521 kb on - strand at 1883.521 kb on - strand at 1883.523 kb on + strand at 1883.597 kb on + strand at 1883.717 kb on + strand, within Atu1906 at 1883.902 kb on + strand, within Atu1906 at 1884.063 kb on + strand, within Atu1906 at 1884.111 kb on + strand, within Atu1906 at 1884.119 kb on - strand, within Atu1906 at 1884.285 kb on - strand, within Atu1906 at 1884.315 kb on + strand at 1884.321 kb on - strand at 1884.474 kb on + strand, within Atu1907 at 1884.691 kb on - strand, within Atu1907 at 1884.728 kb on + strand, within Atu1907 at 1884.729 kb on - strand, within Atu1907 at 1884.803 kb on + strand, within Atu1907 at 1884.844 kb on + strand, within Atu1907 at 1884.844 kb on + strand, within Atu1907 at 1884.956 kb on + strand, within Atu1907 at 1884.987 kb on - strand, within Atu1907 at 1885.016 kb on + strand, within Atu1907 at 1885.016 kb on + strand, within Atu1907 at 1885.024 kb on - strand, within Atu1907 at 1885.024 kb on - strand, within Atu1907 at 1885.034 kb on - strand, within Atu1907 at 1885.056 kb on - strand, within Atu1907 at 1885.219 kb on + strand at 1885.227 kb on - strand at 1885.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,882,729 - +0.1 1,882,774 - Atu1905 0.14 +0.1 1,882,794 - Atu1905 0.16 +0.3 1,882,892 - Atu1905 0.29 +0.1 1,882,926 - Atu1905 0.33 +0.3 1,883,128 + Atu1905 0.59 +0.3 1,883,136 - Atu1905 0.60 -0.0 1,883,205 - Atu1905 0.68 +0.4 1,883,318 + Atu1905 0.83 +0.0 1,883,467 + -0.2 1,883,472 - +0.2 1,883,472 - -0.1 1,883,521 - +0.2 1,883,521 - -0.4 1,883,523 + -0.1 1,883,597 + +0.3 1,883,717 + Atu1906 0.12 -0.9 1,883,902 + Atu1906 0.37 -1.0 1,884,063 + Atu1906 0.58 -0.8 1,884,111 + Atu1906 0.64 -1.1 1,884,119 - Atu1906 0.65 -0.7 1,884,285 - Atu1906 0.87 -0.5 1,884,315 + -0.6 1,884,321 - -1.4 1,884,474 + Atu1907 0.10 -1.0 1,884,691 - Atu1907 0.34 -0.6 1,884,728 + Atu1907 0.38 -0.1 1,884,729 - Atu1907 0.38 -1.7 1,884,803 + Atu1907 0.46 -0.2 1,884,844 + Atu1907 0.50 -0.8 1,884,844 + Atu1907 0.50 -1.6 1,884,956 + Atu1907 0.62 -0.3 1,884,987 - Atu1907 0.65 -0.5 1,885,016 + Atu1907 0.69 -0.9 1,885,016 + Atu1907 0.69 -1.3 1,885,024 - Atu1907 0.69 -0.7 1,885,024 - Atu1907 0.69 -0.8 1,885,034 - Atu1907 0.71 +0.1 1,885,056 - Atu1907 0.73 -0.0 1,885,219 + -0.9 1,885,227 - -0.4 1,885,378 + +0.9
Or see this region's nucleotide sequence