Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1778 and Atu1779 are separated by 3 nucleotides Atu1779 and Atu1780 are separated by 187 nucleotides Atu1780 and Atu1781 are separated by 12 nucleotides Atu1781 and Atu1782 are separated by 27 nucleotides Atu1782 and Atu1783 overlap by 4 nucleotides Atu1783 and Atu1784 are separated by 119 nucleotides
Atu1778: Atu1778 - transcription-repair coupling factor, at 1,762,494 to 1,765,991
Atu1778
Atu1779: Atu1779 - hypothetical protein, at 1,765,995 to 1,766,306
Atu1779
Atu1780: Atu1780 - ATP-dependent DNA helicase, at 1,766,494 to 1,768,599
Atu1780
Atu1781: Atu1781 - hypothetical protein, at 1,768,612 to 1,768,884
Atu1781
Atu1782: Atu1782 - hypothetical protein, at 1,768,912 to 1,769,202
Atu1782
Atu1783: Atu1783 - hypothetical protein, at 1,769,199 to 1,769,444
Atu1783
Atu1784: Atu1784 - hypothetical protein, at 1,769,564 to 1,770,232
Atu1784
Position (kb)
1766
1767
1768
1769 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1765.618 kb on - strand, within Atu1778 at 1765.763 kb on - strand at 1765.795 kb on + strand at 1765.826 kb on + strand at 1765.861 kb on + strand at 1766.035 kb on + strand, within Atu1779 at 1766.316 kb on - strand at 1766.323 kb on - strand at 1766.337 kb on - strand at 1766.466 kb on + strand at 1766.524 kb on + strand at 1766.539 kb on - strand at 1766.544 kb on - strand at 1766.600 kb on - strand at 1766.638 kb on + strand at 1766.950 kb on + strand, within Atu1780 at 1766.958 kb on - strand, within Atu1780 at 1767.059 kb on + strand, within Atu1780 at 1767.087 kb on + strand, within Atu1780 at 1767.235 kb on - strand, within Atu1780 at 1767.235 kb on - strand, within Atu1780 at 1767.278 kb on - strand, within Atu1780 at 1767.325 kb on - strand, within Atu1780 at 1767.349 kb on - strand, within Atu1780 at 1767.499 kb on + strand, within Atu1780 at 1767.499 kb on + strand, within Atu1780 at 1767.507 kb on - strand, within Atu1780 at 1767.551 kb on - strand, within Atu1780 at 1767.558 kb on - strand, within Atu1780 at 1767.705 kb on + strand, within Atu1780 at 1767.768 kb on + strand, within Atu1780 at 1767.797 kb on + strand, within Atu1780 at 1767.797 kb on + strand, within Atu1780 at 1767.809 kb on - strand, within Atu1780 at 1767.863 kb on + strand, within Atu1780 at 1767.931 kb on + strand, within Atu1780 at 1767.948 kb on + strand, within Atu1780 at 1767.986 kb on + strand, within Atu1780 at 1767.996 kb on + strand, within Atu1780 at 1768.043 kb on - strand, within Atu1780 at 1768.054 kb on - strand, within Atu1780 at 1768.211 kb on + strand, within Atu1780 at 1768.278 kb on + strand, within Atu1780 at 1768.281 kb on + strand, within Atu1780 at 1768.284 kb on - strand, within Atu1780 at 1768.291 kb on - strand, within Atu1780 at 1768.375 kb on - strand, within Atu1780 at 1768.447 kb on + strand at 1768.494 kb on - strand at 1768.505 kb on + strand at 1768.529 kb on + strand at 1768.553 kb on + strand at 1768.609 kb on + strand at 1768.609 kb on + strand at 1768.619 kb on + strand at 1768.701 kb on - strand, within Atu1781 at 1768.785 kb on + strand, within Atu1781 at 1768.816 kb on - strand, within Atu1781 at 1768.858 kb on - strand at 1768.863 kb on - strand at 1768.891 kb on - strand at 1768.891 kb on - strand at 1768.905 kb on + strand at 1768.913 kb on - strand at 1768.925 kb on + strand at 1769.045 kb on + strand, within Atu1782 at 1769.120 kb on + strand, within Atu1782 at 1769.128 kb on - strand, within Atu1782 at 1769.164 kb on - strand, within Atu1782 at 1769.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,765,618 - Atu1778 0.89 -0.9 1,765,763 - +0.5 1,765,795 + +0.7 1,765,826 + -0.9 1,765,861 + +0.0 1,766,035 + Atu1779 0.13 -0.1 1,766,316 - -0.6 1,766,323 - +0.7 1,766,337 - -0.2 1,766,466 + +0.4 1,766,524 + -3.5 1,766,539 - -0.4 1,766,544 - -2.5 1,766,600 - -0.7 1,766,638 + -1.7 1,766,950 + Atu1780 0.22 -1.2 1,766,958 - Atu1780 0.22 -1.8 1,767,059 + Atu1780 0.27 +0.2 1,767,087 + Atu1780 0.28 -0.8 1,767,235 - Atu1780 0.35 -1.5 1,767,235 - Atu1780 0.35 -0.8 1,767,278 - Atu1780 0.37 -0.7 1,767,325 - Atu1780 0.39 -0.8 1,767,349 - Atu1780 0.41 -2.1 1,767,499 + Atu1780 0.48 -1.6 1,767,499 + Atu1780 0.48 -0.7 1,767,507 - Atu1780 0.48 -0.3 1,767,551 - Atu1780 0.50 -1.1 1,767,558 - Atu1780 0.51 -0.2 1,767,705 + Atu1780 0.58 +0.1 1,767,768 + Atu1780 0.60 -2.2 1,767,797 + Atu1780 0.62 -2.1 1,767,797 + Atu1780 0.62 +0.6 1,767,809 - Atu1780 0.62 -2.6 1,767,863 + Atu1780 0.65 -0.7 1,767,931 + Atu1780 0.68 -1.7 1,767,948 + Atu1780 0.69 +1.2 1,767,986 + Atu1780 0.71 -1.0 1,767,996 + Atu1780 0.71 -2.7 1,768,043 - Atu1780 0.74 -1.7 1,768,054 - Atu1780 0.74 -0.8 1,768,211 + Atu1780 0.82 -0.1 1,768,278 + Atu1780 0.85 -0.6 1,768,281 + Atu1780 0.85 -1.8 1,768,284 - Atu1780 0.85 -1.7 1,768,291 - Atu1780 0.85 -1.0 1,768,375 - Atu1780 0.89 -1.4 1,768,447 + -0.5 1,768,494 - -0.2 1,768,505 + -1.4 1,768,529 + -0.6 1,768,553 + -0.2 1,768,609 + -0.1 1,768,609 + +0.5 1,768,619 + +0.0 1,768,701 - Atu1781 0.33 -0.1 1,768,785 + Atu1781 0.63 -0.1 1,768,816 - Atu1781 0.75 -0.2 1,768,858 - -1.1 1,768,863 - -0.2 1,768,891 - -0.2 1,768,891 - +1.3 1,768,905 + +0.0 1,768,913 - +0.2 1,768,925 + -0.2 1,769,045 + Atu1782 0.46 +0.3 1,769,120 + Atu1782 0.71 +0.2 1,769,128 - Atu1782 0.74 -0.5 1,769,164 - Atu1782 0.87 -0.5 1,769,541 + -0.4
Or see this region's nucleotide sequence