Strain Fitness in Agrobacterium fabrum C58 around Atu1332

Experiment: R2A with Nitrofurantoin 0.4 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu1330 and Atu1331 are separated by 12 nucleotidesAtu1331 and Atu1332 are separated by 25 nucleotidesAtu1332 and Atu1333 are separated by 125 nucleotides Atu1330: Atu1330 - acetyl-CoA carboxylase, biotin carboxylase, at 1,316,847 to 1,318,193 Atu1330 Atu1331: Atu1331 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 1,318,206 to 1,318,694 Atu1331 Atu1332: Atu1332 - 3-dehydroquinate dehydratase, at 1,318,720 to 1,319,157 Atu1332 Atu1333: Atu1333 - outer membrane protein, at 1,319,283 to 1,320,050 Atu1333 Position (kb) 1318 1319 1320Strain fitness (log2 ratio) -1 0 1at 1319.428 kb on - strand, within Atu1333at 1319.707 kb on + strand, within Atu1333at 1319.820 kb on - strand, within Atu1333at 1319.996 kb on - strandat 1320.026 kb on + strandat 1320.071 kb on + strandat 1320.089 kb on - strandat 1320.148 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL
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1,319,428 - Atu1333 0.19 -0.1
1,319,707 + Atu1333 0.55 -0.5
1,319,820 - Atu1333 0.70 +0.3
1,319,996 - -1.2
1,320,026 + +1.5
1,320,071 + +0.2
1,320,089 - +1.0
1,320,148 + +0.2

Or see this region's nucleotide sequence