Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1126 and Atu1127 are separated by 4 nucleotides Atu1127 and Atu1128 overlap by 1 nucleotides Atu1128 and Atu1129 are separated by 102 nucleotides Atu1129 and Atu1130 are separated by 113 nucleotides
Atu1126: Atu1126 - molybdopterin converting factor, large subunit, at 1,116,007 to 1,116,474
Atu1126
Atu1127: Atu1127 - molybdopterin converting factor small subunit, at 1,116,479 to 1,116,733
Atu1127
Atu1128: Atu1128 - phosphatidylglycerophosphate synthase, at 1,116,733 to 1,117,320
Atu1128
Atu1129: Atu1129 - excinuclease ABC chain C, at 1,117,423 to 1,119,351
Atu1129
Atu1130: Atu1130 - dehydrogenase, at 1,119,465 to 1,120,253
Atu1130
Position (kb)
1117
1118
1119
1120 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1116.587 kb on + strand, within Atu1127 at 1116.595 kb on - strand, within Atu1127 at 1117.651 kb on + strand, within Atu1129 at 1117.777 kb on - strand, within Atu1129 at 1117.829 kb on - strand, within Atu1129 at 1117.843 kb on - strand, within Atu1129 at 1117.843 kb on - strand, within Atu1129 at 1117.880 kb on - strand, within Atu1129 at 1117.957 kb on - strand, within Atu1129 at 1118.160 kb on + strand, within Atu1129 at 1118.168 kb on - strand, within Atu1129 at 1118.399 kb on + strand, within Atu1129 at 1118.407 kb on - strand, within Atu1129 at 1118.417 kb on - strand, within Atu1129 at 1118.428 kb on - strand, within Atu1129 at 1118.493 kb on - strand, within Atu1129 at 1118.658 kb on + strand, within Atu1129 at 1118.674 kb on - strand, within Atu1129 at 1118.795 kb on + strand, within Atu1129 at 1118.802 kb on - strand, within Atu1129 at 1118.808 kb on + strand, within Atu1129 at 1118.857 kb on - strand, within Atu1129 at 1119.096 kb on + strand, within Atu1129 at 1119.174 kb on + strand at 1119.191 kb on + strand at 1119.346 kb on + strand at 1119.354 kb on + strand at 1119.359 kb on + strand at 1119.485 kb on + strand at 1119.493 kb on - strand at 1119.493 kb on - strand at 1119.639 kb on + strand, within Atu1130 at 1119.669 kb on + strand, within Atu1130 at 1119.682 kb on - strand, within Atu1130 at 1119.688 kb on + strand, within Atu1130 at 1119.696 kb on - strand, within Atu1130 at 1119.715 kb on - strand, within Atu1130 at 1119.727 kb on - strand, within Atu1130 at 1119.803 kb on + strand, within Atu1130 at 1119.848 kb on + strand, within Atu1130 at 1119.990 kb on - strand, within Atu1130 at 1120.013 kb on + strand, within Atu1130 at 1120.021 kb on - strand, within Atu1130 at 1120.151 kb on - strand, within Atu1130 at 1120.255 kb on - strand at 1120.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 1,116,587 + Atu1127 0.42 -1.0 1,116,595 - Atu1127 0.45 +0.8 1,117,651 + Atu1129 0.12 -2.8 1,117,777 - Atu1129 0.18 -1.0 1,117,829 - Atu1129 0.21 -2.8 1,117,843 - Atu1129 0.22 -1.2 1,117,843 - Atu1129 0.22 -2.4 1,117,880 - Atu1129 0.24 -2.5 1,117,957 - Atu1129 0.28 +0.7 1,118,160 + Atu1129 0.38 -2.5 1,118,168 - Atu1129 0.39 -1.1 1,118,399 + Atu1129 0.51 -2.6 1,118,407 - Atu1129 0.51 -2.4 1,118,417 - Atu1129 0.52 -5.1 1,118,428 - Atu1129 0.52 -1.4 1,118,493 - Atu1129 0.55 -2.7 1,118,658 + Atu1129 0.64 -2.9 1,118,674 - Atu1129 0.65 -2.9 1,118,795 + Atu1129 0.71 -2.9 1,118,802 - Atu1129 0.71 -1.4 1,118,808 + Atu1129 0.72 -5.5 1,118,857 - Atu1129 0.74 -1.2 1,119,096 + Atu1129 0.87 -3.1 1,119,174 + -5.2 1,119,191 + -4.7 1,119,346 + -0.4 1,119,354 + +1.5 1,119,359 + -0.8 1,119,485 + -1.3 1,119,493 - -0.1 1,119,493 - -1.0 1,119,639 + Atu1130 0.22 +0.1 1,119,669 + Atu1130 0.26 -0.3 1,119,682 - Atu1130 0.28 -0.7 1,119,688 + Atu1130 0.28 -0.3 1,119,696 - Atu1130 0.29 -1.2 1,119,715 - Atu1130 0.32 -0.8 1,119,727 - Atu1130 0.33 -0.2 1,119,803 + Atu1130 0.43 +0.7 1,119,848 + Atu1130 0.49 -1.3 1,119,990 - Atu1130 0.67 +0.5 1,120,013 + Atu1130 0.69 +0.2 1,120,021 - Atu1130 0.70 +0.0 1,120,151 - Atu1130 0.87 -0.1 1,120,255 - +0.3 1,120,292 + -0.1
Or see this region's nucleotide sequence