Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0519 and Atu0520 overlap by 1 nucleotides Atu0520 and Atu0521 overlap by 4 nucleotides Atu0521 and Atu0522 are separated by 14 nucleotides
Atu0519: Atu0519 - protein-glutamate methylesterase, at 509,606 to 510,661
Atu0519
Atu0520: Atu0520 - chemotaxis receiver protein, at 510,661 to 511,050
Atu0520
Atu0521: Atu0521 - methyl-accepting chemotaxis protein, at 511,047 to 511,592
Atu0521
Atu0522: Atu0522 - hypothetical protein, at 511,607 to 511,990
Atu0522
Position (kb) 510
511
512 Strain fitness (log2 ratio) -2
-1
0
1
2 at 509.662 kb on + strand at 509.674 kb on + strand at 509.682 kb on - strand at 509.722 kb on + strand, within Atu0519 at 509.826 kb on - strand, within Atu0519 at 509.937 kb on + strand, within Atu0519 at 509.974 kb on + strand, within Atu0519 at 509.975 kb on + strand, within Atu0519 at 509.982 kb on - strand, within Atu0519 at 510.083 kb on - strand, within Atu0519 at 510.083 kb on - strand, within Atu0519 at 510.126 kb on + strand, within Atu0519 at 510.215 kb on + strand, within Atu0519 at 510.217 kb on + strand, within Atu0519 at 510.283 kb on + strand, within Atu0519 at 510.291 kb on - strand, within Atu0519 at 510.291 kb on - strand, within Atu0519 at 510.296 kb on - strand, within Atu0519 at 510.330 kb on + strand, within Atu0519 at 510.387 kb on + strand, within Atu0519 at 510.441 kb on + strand, within Atu0519 at 510.469 kb on - strand, within Atu0519 at 510.513 kb on + strand, within Atu0519 at 510.513 kb on + strand, within Atu0519 at 510.521 kb on - strand, within Atu0519 at 510.550 kb on - strand, within Atu0519 at 510.572 kb on - strand at 510.579 kb on + strand at 510.588 kb on + strand at 510.596 kb on + strand at 510.693 kb on - strand at 510.731 kb on - strand, within Atu0520 at 510.767 kb on - strand, within Atu0520 at 510.767 kb on - strand, within Atu0520 at 510.865 kb on - strand, within Atu0520 at 510.930 kb on + strand, within Atu0520 at 510.936 kb on + strand, within Atu0520 at 510.938 kb on - strand, within Atu0520 at 511.091 kb on + strand at 511.091 kb on + strand at 511.099 kb on - strand at 511.099 kb on - strand at 511.164 kb on - strand, within Atu0521 at 511.166 kb on + strand, within Atu0521 at 511.198 kb on - strand, within Atu0521 at 511.203 kb on - strand, within Atu0521 at 511.205 kb on + strand, within Atu0521 at 511.241 kb on + strand, within Atu0521 at 511.241 kb on + strand, within Atu0521 at 511.282 kb on + strand, within Atu0521 at 511.365 kb on + strand, within Atu0521 at 511.443 kb on + strand, within Atu0521 at 511.445 kb on - strand, within Atu0521 at 511.473 kb on + strand, within Atu0521 at 511.507 kb on - strand, within Atu0521 at 511.516 kb on + strand, within Atu0521 at 511.564 kb on - strand at 511.564 kb on - strand at 511.637 kb on - strand at 511.637 kb on - strand at 511.736 kb on - strand, within Atu0522 at 511.743 kb on + strand, within Atu0522 at 511.788 kb on + strand, within Atu0522 at 511.789 kb on - strand, within Atu0522 at 511.790 kb on - strand, within Atu0522 at 511.806 kb on + strand, within Atu0522 at 511.850 kb on + strand, within Atu0522 at 511.860 kb on + strand, within Atu0522 at 511.871 kb on - strand, within Atu0522 at 511.891 kb on + strand, within Atu0522 at 511.992 kb on - strand at 511.995 kb on + strand at 512.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 509,662 + +0.4 509,674 + -0.3 509,682 - -1.4 509,722 + Atu0519 0.11 -0.4 509,826 - Atu0519 0.21 +1.8 509,937 + Atu0519 0.31 -0.2 509,974 + Atu0519 0.35 -1.7 509,975 + Atu0519 0.35 -0.7 509,982 - Atu0519 0.36 -0.5 510,083 - Atu0519 0.45 -0.1 510,083 - Atu0519 0.45 -1.5 510,126 + Atu0519 0.49 -1.5 510,215 + Atu0519 0.58 +2.1 510,217 + Atu0519 0.58 -0.3 510,283 + Atu0519 0.64 -0.4 510,291 - Atu0519 0.65 +0.1 510,291 - Atu0519 0.65 -0.8 510,296 - Atu0519 0.65 -0.6 510,330 + Atu0519 0.69 -0.5 510,387 + Atu0519 0.74 -0.8 510,441 + Atu0519 0.79 -0.3 510,469 - Atu0519 0.82 -0.1 510,513 + Atu0519 0.86 -0.8 510,513 + Atu0519 0.86 -0.1 510,521 - Atu0519 0.87 -0.8 510,550 - Atu0519 0.89 -0.3 510,572 - -1.3 510,579 + -0.4 510,588 + -1.2 510,596 + -0.7 510,693 - -0.8 510,731 - Atu0520 0.18 -0.0 510,767 - Atu0520 0.27 -1.1 510,767 - Atu0520 0.27 -0.2 510,865 - Atu0520 0.52 -0.8 510,930 + Atu0520 0.69 -0.5 510,936 + Atu0520 0.71 -0.5 510,938 - Atu0520 0.71 -0.6 511,091 + +0.1 511,091 + -0.4 511,099 - +0.2 511,099 - -0.4 511,164 - Atu0521 0.21 +0.6 511,166 + Atu0521 0.22 -0.4 511,198 - Atu0521 0.28 -0.6 511,203 - Atu0521 0.29 -0.3 511,205 + Atu0521 0.29 -0.1 511,241 + Atu0521 0.36 +0.2 511,241 + Atu0521 0.36 +0.2 511,282 + Atu0521 0.43 -0.1 511,365 + Atu0521 0.58 -0.3 511,443 + Atu0521 0.73 -0.4 511,445 - Atu0521 0.73 -0.7 511,473 + Atu0521 0.78 +0.0 511,507 - Atu0521 0.84 -0.5 511,516 + Atu0521 0.86 +0.7 511,564 - +0.0 511,564 - -0.3 511,637 - -0.9 511,637 - +0.1 511,736 - Atu0522 0.34 +1.4 511,743 + Atu0522 0.35 -0.1 511,788 + Atu0522 0.47 +0.9 511,789 - Atu0522 0.47 +0.4 511,790 - Atu0522 0.48 -2.1 511,806 + Atu0522 0.52 -0.3 511,850 + Atu0522 0.63 -0.1 511,860 + Atu0522 0.66 +0.1 511,871 - Atu0522 0.69 +0.3 511,891 + Atu0522 0.74 -1.4 511,992 - -0.0 511,995 + +0.6 512,010 + -0.9
Or see this region's nucleotide sequence