Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0517 and Atu0518 are separated by 13 nucleotides Atu0518 and Atu0519 overlap by 4 nucleotides
Atu0517: Atu0517 - Chemotaxis protein histidine kinase, at 506,474 to 508,687
Atu0517
Atu0518: Atu0518 - chemotaxis methyltransferase, at 508,701 to 509,609
Atu0518
Atu0519: Atu0519 - protein-glutamate methylesterase, at 509,606 to 510,661
Atu0519
Position (kb)
508
509
510 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 507.728 kb on + strand, within Atu0517 at 507.759 kb on - strand, within Atu0517 at 507.778 kb on - strand, within Atu0517 at 507.797 kb on + strand, within Atu0517 at 507.826 kb on - strand, within Atu0517 at 507.842 kb on + strand, within Atu0517 at 507.850 kb on - strand, within Atu0517 at 507.850 kb on - strand, within Atu0517 at 507.907 kb on + strand, within Atu0517 at 507.914 kb on - strand, within Atu0517 at 507.925 kb on + strand, within Atu0517 at 507.943 kb on - strand, within Atu0517 at 508.042 kb on - strand, within Atu0517 at 508.146 kb on + strand, within Atu0517 at 508.235 kb on - strand, within Atu0517 at 508.245 kb on + strand, within Atu0517 at 508.245 kb on + strand, within Atu0517 at 508.250 kb on - strand, within Atu0517 at 508.297 kb on + strand, within Atu0517 at 508.335 kb on - strand, within Atu0517 at 508.341 kb on + strand, within Atu0517 at 508.366 kb on + strand, within Atu0517 at 508.429 kb on - strand, within Atu0517 at 508.435 kb on + strand, within Atu0517 at 508.442 kb on + strand, within Atu0517 at 508.479 kb on + strand at 508.591 kb on + strand at 508.593 kb on + strand at 508.686 kb on + strand at 508.693 kb on + strand at 508.813 kb on - strand, within Atu0518 at 508.845 kb on - strand, within Atu0518 at 508.928 kb on - strand, within Atu0518 at 509.027 kb on - strand, within Atu0518 at 509.034 kb on + strand, within Atu0518 at 509.076 kb on + strand, within Atu0518 at 509.211 kb on + strand, within Atu0518 at 509.224 kb on - strand, within Atu0518 at 509.268 kb on - strand, within Atu0518 at 509.350 kb on + strand, within Atu0518 at 509.357 kb on - strand, within Atu0518 at 509.421 kb on + strand, within Atu0518 at 509.450 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.544 kb on + strand at 509.603 kb on + strand at 509.611 kb on - strand at 509.662 kb on + strand at 509.674 kb on + strand at 509.682 kb on - strand at 509.722 kb on + strand, within Atu0519 at 509.826 kb on - strand, within Atu0519 at 509.937 kb on + strand, within Atu0519 at 509.974 kb on + strand, within Atu0519 at 509.975 kb on + strand, within Atu0519 at 509.982 kb on - strand, within Atu0519 at 510.083 kb on - strand, within Atu0519 at 510.083 kb on - strand, within Atu0519 at 510.126 kb on + strand, within Atu0519 at 510.215 kb on + strand, within Atu0519 at 510.217 kb on + strand, within Atu0519 at 510.283 kb on + strand, within Atu0519 at 510.291 kb on - strand, within Atu0519 at 510.291 kb on - strand, within Atu0519 at 510.296 kb on - strand, within Atu0519 at 510.330 kb on + strand, within Atu0519 at 510.387 kb on + strand, within Atu0519 at 510.441 kb on + strand, within Atu0519 at 510.469 kb on - strand, within Atu0519 at 510.513 kb on + strand, within Atu0519 at 510.513 kb on + strand, within Atu0519 at 510.521 kb on - strand, within Atu0519 at 510.550 kb on - strand, within Atu0519 at 510.572 kb on - strand at 510.579 kb on + strand at 510.588 kb on + strand at 510.596 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 507,728 + Atu0517 0.57 -0.3 507,759 - Atu0517 0.58 -0.8 507,778 - Atu0517 0.59 +0.0 507,797 + Atu0517 0.60 -0.9 507,826 - Atu0517 0.61 -0.8 507,842 + Atu0517 0.62 +1.7 507,850 - Atu0517 0.62 -1.1 507,850 - Atu0517 0.62 -0.9 507,907 + Atu0517 0.65 +0.3 507,914 - Atu0517 0.65 -0.6 507,925 + Atu0517 0.66 -1.2 507,943 - Atu0517 0.66 -0.3 508,042 - Atu0517 0.71 -1.1 508,146 + Atu0517 0.76 -0.8 508,235 - Atu0517 0.80 -0.9 508,245 + Atu0517 0.80 -0.8 508,245 + Atu0517 0.80 -1.0 508,250 - Atu0517 0.80 +0.1 508,297 + Atu0517 0.82 -1.1 508,335 - Atu0517 0.84 -0.7 508,341 + Atu0517 0.84 -1.7 508,366 + Atu0517 0.85 +0.2 508,429 - Atu0517 0.88 +0.1 508,435 + Atu0517 0.89 -0.8 508,442 + Atu0517 0.89 +0.4 508,479 + -0.6 508,591 + -1.3 508,593 + -0.8 508,686 + +0.3 508,693 + -0.1 508,813 - Atu0518 0.12 -0.7 508,845 - Atu0518 0.16 +0.7 508,928 - Atu0518 0.25 -0.6 509,027 - Atu0518 0.36 -0.6 509,034 + Atu0518 0.37 -1.4 509,076 + Atu0518 0.41 -0.5 509,211 + Atu0518 0.56 -0.5 509,224 - Atu0518 0.58 -0.2 509,268 - Atu0518 0.62 -1.0 509,350 + Atu0518 0.71 +0.8 509,357 - Atu0518 0.72 -0.7 509,421 + Atu0518 0.79 -1.0 509,450 + Atu0518 0.82 -0.5 509,498 + Atu0518 0.88 -1.0 509,498 + Atu0518 0.88 -0.5 509,498 + Atu0518 0.88 -0.5 509,498 + Atu0518 0.88 +1.6 509,498 + Atu0518 0.88 -1.3 509,506 - Atu0518 0.89 +0.4 509,506 - Atu0518 0.89 -0.2 509,506 - Atu0518 0.89 -0.2 509,544 + -2.5 509,603 + -0.1 509,611 - -0.6 509,662 + +0.4 509,674 + -0.3 509,682 - -1.4 509,722 + Atu0519 0.11 -0.4 509,826 - Atu0519 0.21 +1.8 509,937 + Atu0519 0.31 -0.2 509,974 + Atu0519 0.35 -1.7 509,975 + Atu0519 0.35 -0.7 509,982 - Atu0519 0.36 -0.5 510,083 - Atu0519 0.45 -0.1 510,083 - Atu0519 0.45 -1.5 510,126 + Atu0519 0.49 -1.5 510,215 + Atu0519 0.58 +2.1 510,217 + Atu0519 0.58 -0.3 510,283 + Atu0519 0.64 -0.4 510,291 - Atu0519 0.65 +0.1 510,291 - Atu0519 0.65 -0.8 510,296 - Atu0519 0.65 -0.6 510,330 + Atu0519 0.69 -0.5 510,387 + Atu0519 0.74 -0.8 510,441 + Atu0519 0.79 -0.3 510,469 - Atu0519 0.82 -0.1 510,513 + Atu0519 0.86 -0.8 510,513 + Atu0519 0.86 -0.1 510,521 - Atu0519 0.87 -0.8 510,550 - Atu0519 0.89 -0.3 510,572 - -1.3 510,579 + -0.4 510,588 + -1.2 510,596 + -0.7
Or see this region's nucleotide sequence