Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0514 and Atu0515 overlap by 1 nucleotides Atu0515 and Atu0516 overlap by 4 nucleotides Atu0516 and Atu0517 are separated by 76 nucleotides Atu0517 and Atu0518 are separated by 13 nucleotides Atu0518 and Atu0519 overlap by 4 nucleotides
Atu0514: Atu0514 - methyl-accepting chemotaxis protein, at 504,030 to 505,736
Atu0514
Atu0515: Atu0515 - hypothetical protein, at 505,736 to 506,035
Atu0515
Atu0516: Atu0516 - chemotaxis receiver protein, at 506,032 to 506,397
Atu0516
Atu0517: Atu0517 - Chemotaxis protein histidine kinase, at 506,474 to 508,687
Atu0517
Atu0518: Atu0518 - chemotaxis methyltransferase, at 508,701 to 509,609
Atu0518
Atu0519: Atu0519 - protein-glutamate methylesterase, at 509,606 to 510,661
Atu0519
Position (kb)
506
507
508
509 Strain fitness (log2 ratio)
-2
-1
0
1 at 505.526 kb on + strand, within Atu0514 at 505.559 kb on + strand, within Atu0514 at 505.720 kb on + strand at 505.721 kb on + strand at 505.799 kb on + strand, within Atu0515 at 505.813 kb on - strand, within Atu0515 at 505.863 kb on + strand, within Atu0515 at 505.863 kb on + strand, within Atu0515 at 505.977 kb on - strand, within Atu0515 at 506.014 kb on + strand at 506.144 kb on + strand, within Atu0516 at 506.171 kb on - strand, within Atu0516 at 506.176 kb on - strand, within Atu0516 at 506.176 kb on - strand, within Atu0516 at 506.314 kb on + strand, within Atu0516 at 506.314 kb on + strand, within Atu0516 at 506.347 kb on + strand, within Atu0516 at 506.382 kb on + strand at 506.390 kb on - strand at 506.454 kb on + strand at 506.467 kb on - strand at 506.621 kb on + strand at 506.654 kb on - strand at 506.713 kb on - strand, within Atu0517 at 506.785 kb on + strand, within Atu0517 at 506.793 kb on - strand, within Atu0517 at 506.793 kb on - strand, within Atu0517 at 506.866 kb on + strand, within Atu0517 at 506.900 kb on + strand, within Atu0517 at 506.950 kb on + strand, within Atu0517 at 506.959 kb on + strand, within Atu0517 at 507.002 kb on - strand, within Atu0517 at 507.044 kb on + strand, within Atu0517 at 507.213 kb on + strand, within Atu0517 at 507.248 kb on - strand, within Atu0517 at 507.267 kb on + strand, within Atu0517 at 507.304 kb on - strand, within Atu0517 at 507.434 kb on - strand, within Atu0517 at 507.456 kb on - strand, within Atu0517 at 507.617 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.650 kb on + strand, within Atu0517 at 507.658 kb on - strand, within Atu0517 at 507.658 kb on - strand, within Atu0517 at 507.728 kb on + strand, within Atu0517 at 507.759 kb on - strand, within Atu0517 at 507.778 kb on - strand, within Atu0517 at 507.797 kb on + strand, within Atu0517 at 507.826 kb on - strand, within Atu0517 at 507.842 kb on + strand, within Atu0517 at 507.850 kb on - strand, within Atu0517 at 507.850 kb on - strand, within Atu0517 at 507.907 kb on + strand, within Atu0517 at 507.914 kb on - strand, within Atu0517 at 507.925 kb on + strand, within Atu0517 at 507.943 kb on - strand, within Atu0517 at 508.042 kb on - strand, within Atu0517 at 508.146 kb on + strand, within Atu0517 at 508.235 kb on - strand, within Atu0517 at 508.245 kb on + strand, within Atu0517 at 508.245 kb on + strand, within Atu0517 at 508.250 kb on - strand, within Atu0517 at 508.297 kb on + strand, within Atu0517 at 508.335 kb on - strand, within Atu0517 at 508.341 kb on + strand, within Atu0517 at 508.366 kb on + strand, within Atu0517 at 508.429 kb on - strand, within Atu0517 at 508.435 kb on + strand, within Atu0517 at 508.442 kb on + strand, within Atu0517 at 508.479 kb on + strand at 508.591 kb on + strand at 508.593 kb on + strand at 508.686 kb on + strand at 508.693 kb on + strand at 508.813 kb on - strand, within Atu0518 at 508.845 kb on - strand, within Atu0518 at 508.928 kb on - strand, within Atu0518 at 509.027 kb on - strand, within Atu0518 at 509.034 kb on + strand, within Atu0518 at 509.076 kb on + strand, within Atu0518 at 509.211 kb on + strand, within Atu0518 at 509.224 kb on - strand, within Atu0518 at 509.268 kb on - strand, within Atu0518 at 509.350 kb on + strand, within Atu0518 at 509.357 kb on - strand, within Atu0518 at 509.421 kb on + strand, within Atu0518 at 509.450 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.498 kb on + strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.506 kb on - strand, within Atu0518 at 509.544 kb on + strand at 509.603 kb on + strand at 509.611 kb on - strand at 509.662 kb on + strand at 509.674 kb on + strand at 509.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 505,526 + Atu0514 0.88 -0.1 505,559 + Atu0514 0.90 -0.5 505,720 + -0.1 505,721 + -0.1 505,799 + Atu0515 0.21 -0.3 505,813 - Atu0515 0.26 -0.8 505,863 + Atu0515 0.42 -0.5 505,863 + Atu0515 0.42 -0.8 505,977 - Atu0515 0.80 -0.6 506,014 + +0.6 506,144 + Atu0516 0.31 +0.6 506,171 - Atu0516 0.38 -0.8 506,176 - Atu0516 0.39 -1.2 506,176 - Atu0516 0.39 -0.9 506,314 + Atu0516 0.77 -0.8 506,314 + Atu0516 0.77 -0.7 506,347 + Atu0516 0.86 -0.7 506,382 + -1.3 506,390 - -1.0 506,454 + +0.1 506,467 - -0.8 506,621 + -1.1 506,654 - -0.3 506,713 - Atu0517 0.11 -1.8 506,785 + Atu0517 0.14 -0.4 506,793 - Atu0517 0.14 -0.1 506,793 - Atu0517 0.14 +0.1 506,866 + Atu0517 0.18 -0.6 506,900 + Atu0517 0.19 +0.4 506,950 + Atu0517 0.21 -0.2 506,959 + Atu0517 0.22 -0.9 507,002 - Atu0517 0.24 -1.0 507,044 + Atu0517 0.26 -0.7 507,213 + Atu0517 0.33 +0.2 507,248 - Atu0517 0.35 -1.1 507,267 + Atu0517 0.36 -0.0 507,304 - Atu0517 0.37 -2.0 507,434 - Atu0517 0.43 -0.0 507,456 - Atu0517 0.44 -0.5 507,617 + Atu0517 0.52 -1.0 507,650 + Atu0517 0.53 -0.5 507,650 + Atu0517 0.53 -0.4 507,650 + Atu0517 0.53 -1.0 507,650 + Atu0517 0.53 -0.6 507,650 + Atu0517 0.53 -1.3 507,650 + Atu0517 0.53 +0.5 507,658 - Atu0517 0.53 -0.6 507,658 - Atu0517 0.53 -0.5 507,728 + Atu0517 0.57 -0.3 507,759 - Atu0517 0.58 -0.8 507,778 - Atu0517 0.59 +0.0 507,797 + Atu0517 0.60 -0.9 507,826 - Atu0517 0.61 -0.8 507,842 + Atu0517 0.62 +1.7 507,850 - Atu0517 0.62 -1.1 507,850 - Atu0517 0.62 -0.9 507,907 + Atu0517 0.65 +0.3 507,914 - Atu0517 0.65 -0.6 507,925 + Atu0517 0.66 -1.2 507,943 - Atu0517 0.66 -0.3 508,042 - Atu0517 0.71 -1.1 508,146 + Atu0517 0.76 -0.8 508,235 - Atu0517 0.80 -0.9 508,245 + Atu0517 0.80 -0.8 508,245 + Atu0517 0.80 -1.0 508,250 - Atu0517 0.80 +0.1 508,297 + Atu0517 0.82 -1.1 508,335 - Atu0517 0.84 -0.7 508,341 + Atu0517 0.84 -1.7 508,366 + Atu0517 0.85 +0.2 508,429 - Atu0517 0.88 +0.1 508,435 + Atu0517 0.89 -0.8 508,442 + Atu0517 0.89 +0.4 508,479 + -0.6 508,591 + -1.3 508,593 + -0.8 508,686 + +0.3 508,693 + -0.1 508,813 - Atu0518 0.12 -0.7 508,845 - Atu0518 0.16 +0.7 508,928 - Atu0518 0.25 -0.6 509,027 - Atu0518 0.36 -0.6 509,034 + Atu0518 0.37 -1.4 509,076 + Atu0518 0.41 -0.5 509,211 + Atu0518 0.56 -0.5 509,224 - Atu0518 0.58 -0.2 509,268 - Atu0518 0.62 -1.0 509,350 + Atu0518 0.71 +0.8 509,357 - Atu0518 0.72 -0.7 509,421 + Atu0518 0.79 -1.0 509,450 + Atu0518 0.82 -0.5 509,498 + Atu0518 0.88 -1.0 509,498 + Atu0518 0.88 -0.5 509,498 + Atu0518 0.88 -0.5 509,498 + Atu0518 0.88 +1.6 509,498 + Atu0518 0.88 -1.3 509,506 - Atu0518 0.89 +0.4 509,506 - Atu0518 0.89 -0.2 509,506 - Atu0518 0.89 -0.2 509,544 + -2.5 509,603 + -0.1 509,611 - -0.6 509,662 + +0.4 509,674 + -0.3 509,682 - -1.4
Or see this region's nucleotide sequence