Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu8131 and Atu0513 are separated by 105 nucleotides Atu0513 and Atu0514 are separated by 404 nucleotides Atu0514 and Atu0515 overlap by 1 nucleotides Atu0515 and Atu0516 overlap by 4 nucleotides Atu0516 and Atu0517 are separated by 76 nucleotides
Atu8131: Atu8131 - pH adaption potassium efflux system protein, at 500,224 to 503,142
Atu8131
Atu0513: Atu0513 - hypothetical protein, at 503,248 to 503,625
Atu0513
Atu0514: Atu0514 - methyl-accepting chemotaxis protein, at 504,030 to 505,736
Atu0514
Atu0515: Atu0515 - hypothetical protein, at 505,736 to 506,035
Atu0515
Atu0516: Atu0516 - chemotaxis receiver protein, at 506,032 to 506,397
Atu0516
Atu0517: Atu0517 - Chemotaxis protein histidine kinase, at 506,474 to 508,687
Atu0517
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1 at 503.049 kb on - strand at 503.079 kb on - strand at 503.165 kb on + strand at 503.194 kb on - strand at 503.260 kb on + strand at 503.285 kb on - strand at 503.382 kb on - strand, within Atu0513 at 503.420 kb on + strand, within Atu0513 at 503.420 kb on + strand, within Atu0513 at 503.437 kb on + strand, within Atu0513 at 503.465 kb on - strand, within Atu0513 at 503.465 kb on - strand, within Atu0513 at 503.478 kb on - strand, within Atu0513 at 503.507 kb on + strand, within Atu0513 at 503.507 kb on + strand, within Atu0513 at 503.515 kb on - strand, within Atu0513 at 503.663 kb on + strand at 503.671 kb on - strand at 503.671 kb on - strand at 503.671 kb on - strand at 503.671 kb on - strand at 503.722 kb on + strand at 503.730 kb on - strand at 503.730 kb on - strand at 503.952 kb on + strand at 503.958 kb on - strand at 503.997 kb on + strand at 504.015 kb on + strand at 504.123 kb on - strand at 504.158 kb on - strand at 504.205 kb on + strand, within Atu0514 at 504.251 kb on + strand, within Atu0514 at 504.251 kb on + strand, within Atu0514 at 504.271 kb on - strand, within Atu0514 at 504.285 kb on + strand, within Atu0514 at 504.302 kb on + strand, within Atu0514 at 504.371 kb on - strand, within Atu0514 at 504.384 kb on + strand, within Atu0514 at 504.384 kb on + strand, within Atu0514 at 504.425 kb on + strand, within Atu0514 at 504.425 kb on + strand, within Atu0514 at 504.468 kb on - strand, within Atu0514 at 504.544 kb on + strand, within Atu0514 at 504.551 kb on - strand, within Atu0514 at 504.684 kb on + strand, within Atu0514 at 504.684 kb on + strand, within Atu0514 at 504.784 kb on - strand, within Atu0514 at 504.864 kb on + strand, within Atu0514 at 504.874 kb on + strand, within Atu0514 at 504.882 kb on - strand, within Atu0514 at 504.882 kb on - strand, within Atu0514 at 504.945 kb on - strand, within Atu0514 at 504.981 kb on - strand, within Atu0514 at 505.144 kb on - strand, within Atu0514 at 505.215 kb on + strand, within Atu0514 at 505.439 kb on - strand, within Atu0514 at 505.526 kb on + strand, within Atu0514 at 505.559 kb on + strand, within Atu0514 at 505.720 kb on + strand at 505.721 kb on + strand at 505.799 kb on + strand, within Atu0515 at 505.813 kb on - strand, within Atu0515 at 505.863 kb on + strand, within Atu0515 at 505.863 kb on + strand, within Atu0515 at 505.977 kb on - strand, within Atu0515 at 506.014 kb on + strand at 506.144 kb on + strand, within Atu0516 at 506.171 kb on - strand, within Atu0516 at 506.176 kb on - strand, within Atu0516 at 506.176 kb on - strand, within Atu0516 at 506.314 kb on + strand, within Atu0516 at 506.314 kb on + strand, within Atu0516 at 506.347 kb on + strand, within Atu0516 at 506.382 kb on + strand at 506.390 kb on - strand at 506.454 kb on + strand at 506.467 kb on - strand at 506.621 kb on + strand at 506.654 kb on - strand at 506.713 kb on - strand, within Atu0517
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 503,049 - +0.8 503,079 - -0.1 503,165 + +0.3 503,194 - -0.2 503,260 + -0.1 503,285 - +1.3 503,382 - Atu0513 0.35 +1.7 503,420 + Atu0513 0.46 -0.1 503,420 + Atu0513 0.46 +0.2 503,437 + Atu0513 0.50 -0.2 503,465 - Atu0513 0.57 -0.1 503,465 - Atu0513 0.57 -0.2 503,478 - Atu0513 0.61 +0.0 503,507 + Atu0513 0.69 -0.4 503,507 + Atu0513 0.69 -0.7 503,515 - Atu0513 0.71 +0.5 503,663 + +1.3 503,671 - +0.8 503,671 - -0.3 503,671 - -0.0 503,671 - +0.1 503,722 + +0.8 503,730 - -0.2 503,730 - -1.6 503,952 + -1.5 503,958 - -0.6 503,997 + -0.3 504,015 + +0.2 504,123 - -0.3 504,158 - +0.1 504,205 + Atu0514 0.10 -0.4 504,251 + Atu0514 0.13 +0.1 504,251 + Atu0514 0.13 -0.5 504,271 - Atu0514 0.14 -0.4 504,285 + Atu0514 0.15 -0.9 504,302 + Atu0514 0.16 -0.6 504,371 - Atu0514 0.20 -0.8 504,384 + Atu0514 0.21 +0.9 504,384 + Atu0514 0.21 -1.0 504,425 + Atu0514 0.23 -0.8 504,425 + Atu0514 0.23 -1.1 504,468 - Atu0514 0.26 -0.9 504,544 + Atu0514 0.30 -1.2 504,551 - Atu0514 0.31 -0.8 504,684 + Atu0514 0.38 -1.4 504,684 + Atu0514 0.38 -1.0 504,784 - Atu0514 0.44 -1.7 504,864 + Atu0514 0.49 -1.6 504,874 + Atu0514 0.49 -0.7 504,882 - Atu0514 0.50 -1.3 504,882 - Atu0514 0.50 -1.4 504,945 - Atu0514 0.54 +0.0 504,981 - Atu0514 0.56 -0.7 505,144 - Atu0514 0.65 -0.2 505,215 + Atu0514 0.69 +0.5 505,439 - Atu0514 0.83 -1.0 505,526 + Atu0514 0.88 -0.1 505,559 + Atu0514 0.90 -0.5 505,720 + -0.1 505,721 + -0.1 505,799 + Atu0515 0.21 -0.3 505,813 - Atu0515 0.26 -0.8 505,863 + Atu0515 0.42 -0.5 505,863 + Atu0515 0.42 -0.8 505,977 - Atu0515 0.80 -0.6 506,014 + +0.6 506,144 + Atu0516 0.31 +0.6 506,171 - Atu0516 0.38 -0.8 506,176 - Atu0516 0.39 -1.2 506,176 - Atu0516 0.39 -0.9 506,314 + Atu0516 0.77 -0.8 506,314 + Atu0516 0.77 -0.7 506,347 + Atu0516 0.86 -0.7 506,382 + -1.3 506,390 - -1.0 506,454 + +0.1 506,467 - -0.8 506,621 + -1.1 506,654 - -0.3 506,713 - Atu0517 0.11 -1.8
Or see this region's nucleotide sequence